化学信息：
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化学名
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3-(benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide
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简称
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RS1
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别名
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RS-1, RS 1
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中文名
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N/A
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化学式
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C20H16Br2N2O3S
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分子量
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524.23
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CAS号
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312756-74-4
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纯度
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98%
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溶剂/溶解度
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Water<1mg/ml; DMSO100mg/ml; Ethanol<1mg/ml
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溶液配制
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5mg加入0.95ml DMSO，或者每5.24mg加入1ml DMSO，配制成10mM溶液。SF1069-10mM用DMSO配制。
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生物信息：
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产品描述
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RS-1 is a RAD51-stimulatory compound, which increases the DNA binding activity of RAD51. It is an HDR(homology-directed repair) enhancer.
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信号通路
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Others
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靶点
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RAD51
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－
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－
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－
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－
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IC50
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－
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－
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－
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－
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－
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体外研究
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RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this small molecule enhance DNA binding and result in longer protein–DNA complex lengths. In addition, RS-1 stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity.
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体内研究
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N/A
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临床实验
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N/A
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特征
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N/A
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相关实验数据(此数据来自于公开文献，碧云天并不保证其有效性)：
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酶活性检测实验
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方法
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N/A
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细胞实验
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细胞系
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Early-passage neonatal human dermal fibroblasts
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浓度
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1-7.5μM
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处理时间
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24h
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方法
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Early-passage neonatal human dermal fibroblasts were maintained in Medium 106 supplemented with low serum growth supplement. Cell-survival experiments were performed using a sulforhodamine B assay. Cells are incubated for 24h in cisplatin with or without RS-1 and are subsequently incubated for an additional 6 days in normal media lacking both agents. Sulforhodamine B-stained cells are quantified with a Synergy plate reader.
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动物实验
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动物模型
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N/A
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配制
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N/A
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剂量
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N/A
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给药方式
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N/A
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参考文献：
1. Jayathilaka K, et al. Proc Natl Acad Sci USA. 2008, 105(41), 15848-53.
2. Song J, et al. Nat Commun. 2016, 7, 10548.
包装清单：
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产品编号
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产品名称
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包装
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SF1069-10mM
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RS1 (RAD51激动剂)
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10mM×0.2ml
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SF1069-5mg
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RS1 (RAD51激动剂)
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5mg
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SF1069-25mg
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RS1 (RAD51激动剂)
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25mg
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—
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说明书
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1份
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保存条件：
-20℃保存，至少一年有效。5mg和25mg包装也可室温保存，至少6个月有效。如果溶于非DMSO溶剂，建议分装后-80℃保存，预计6个月内有效。
注意事项：
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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