Fig. 1. Western blot analysis of Beyotime antibody (KO Validated). Secondary antibody: BHRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: BQuickBlock™ Blocking Buffer (P0231). Detection: BBeyoECL Star (P0018A). Exposure time:1s.

Fig. 2 Western blot analysis of extracts of various cell lines, using Beyotime antibody(KO Validated). Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s.

Fig. 3. Immunohistochemistry of paraffin-embedded human mammary cancer using SIRT1 antibody at dilution of 1:100 (a) and human colon using Fatty Acid Synthase antibody at dilution of 1:100 (b)(40x lens) (40x lens).

Fig. 4. Immunofluorescence analysis of U2OS cells using MAPK9 antibody(a) or HeLa cells using EGFR antibody（b）. Blue: DAPI for nuclear staining.