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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2819-50μl | GAPDH Mouse Monoclonal Antibody | 50μl | 299.00元 |
| AF2819-200μl | GAPDH Mouse Monoclonal Antibody | 200μl | 899.00元 |
| AF2819-1ml | GAPDH Mouse Monoclonal Antibody | 1ml | 3528.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IF, IHC, ICC | All | 31/36kDa | 36kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | - | 1:50-1:100 | 1:50-1:100 | 1:50-1:100 | - | - | - |
抗体详细信息如下:
| About this Antibody | |
| Name | GAPDH Mouse Monoclonal Antibody |
| Category | Mouse Monoclonal Antibody (mAb); Primary antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | HeLa, Jurkat, 293T, NIH/3T3, Raw 264.7, Mouse brain, Rat brain, Rat heart, Rat liver |
| Cellular location | Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | Synthetic peptide CRLEKPAKYDDIKK coupled to KLH |
| Sequence | CRLEKPAKYDDIKK |
| Gene ID | 2597 |
| SwissProt | P04406 |
| Synonyms | GAPDH; G3PD; GAPD; HEL-S-162eP; glyceraldehyde-3-phosphate dehydrogenase |
| Category | Metabolism |
| Background | GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. |
| 产品编号 | 产品名称 | 包装 |
| AF2819-50µl | GAPDH Mouse Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2819-200µl | GAPDH Mouse Monoclonal Antibody | 200µl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2819-1ml | GAPDH Mouse Monoclonal Antibody | 1ml |
| AZ502 | Western一抗稀释液 | AZ500×2 |
| — | 说明书 | 1份 |
GAPDH Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| AF2811 | β-Actin Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2815 | β-Actin Mouse Monoclonal Antibody(HRP Conjugated) | 50μl/200μl/1ml |
| AF2819 | GAPDH Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2823 | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2827 | α-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2831 | α-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2835 | β-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2839 | β-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
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