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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AG8025 | Na,K-ATPase α1 Rabbit Monoclonal Antibody | 50μl | 498.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | WB, IHC, FC, IP | H, M, R | 113kDa | 100kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; Ar, Arabidopsis; B, Bovine; C, Chicken; Cerastium, Ce; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Ph, Pharbitis; Pg, Pig; Rb, Rabbit; Rc, Rice; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:1,000-1:2,000 | 1:50-1:100 | - | 1:50-1:100 | - | 1:50-1:100 | - | - |
抗体详细信息如下:
| About this Antibody | |
| Name | Na,K-ATPase α1 Rabbit Monoclonal Antibody |
| Category | Recombinant Rabbit Monoclonal Antibody (mAb); Primary antibody; 内参抗体 |
| Isotype | IgG |
| Purification method | Protein A affinity purification |
| Positive samples | - |
| Cellular location | Membrane |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | A synthetic peptide corresponding to residues aa200-300 of human GAPDH was used as an immunogen |
| Sequence | - |
| Gene ID | 476 |
| SwissProt | P05023 |
| Synonyms | CMT2DD; HOMGSMR2; ATP1A1 |
| Category | Cytoskeletal Signaling |
| Background | The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit. Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase, Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA. All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule. Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit. Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors. |
| 产品编号 | 产品名称 | 包装 |
| AG8025 | Na,K-ATPase α1 Rabbit Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
Na,K-ATPase α1 Rabbit Monoclonal Antibody -20℃保存，需避免反复冻融。Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000×g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
All lanes: Anti-NaK ATPase antibody at 1:1,000 dilution
Predicted MW: 113 kDa
Observed MW: 100 kDa
Lane 1: 293
Lane 2: Hela
Lane 3: MCF-7
Lane 4: Raji
Lane 5: TT
Lane 6: HCT-116
Lysate at 10 μg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 20s
NaK ATPase was immunoprecipitated from 0.4mg of TT whole cell lysate with NaK ATPase Rabbit Monoclonal Antibody at 1:50 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: NaK ATPase Rabbit Monoclonal Antibody IP in TT whole cell lysate
Lane 2: PBS instead of NaK ATPase Rabbit Monoclonal Antibody in TT whole cell lysate
Lane 3: TT whole cell lysate, 10μg (input)
Exposure: 120s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrium tissue labelling NaK ATPase with NaK ATPase Rabbit Monoclonal Antibody at 1:100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Overlay histogram showing HeLa cells stained with NaK ATPase Rabbit Monoclonal Antibody (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% Triton X-100 for 15 min. The cells were then incubated in the antibody (NaK ATPase Rabbit Monoclonal Antibody, 1:100 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
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