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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| L03655 | GNAS基因敲除质粒 | 5µg | 999.00元 |
| L03656 | GNAS基因敲除慢病毒 | 10^8 TU | 6999.00元 |
| L03657 | GNAS基因敲除HEK293T细胞 | 1支/瓶 | 7999.00元 |
| L03658 | GNAS基因敲除HEK293T细胞RIPA裂解液 | 100µg | 1258.00元 |
| L03659 | GNAS基因敲除HEK293T细胞Trizol裂解液 | 500µl | 1258.00元 |
pLenti-GNAS-sgRNA (GNAS基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。
本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。
本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。
碧云天同时提供基于CRISPR/Cas9技术的GNAS基因敲除的质粒(L03655 pLenti-GNAS-sgRNA)、慢病毒(L03656 GNAS Knockout Lentivirus)、HEK293T细胞(L03657 GNAS Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L03658 GNAS Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L03659 GNAS Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
GNAS基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | GNAS | 2778 | BC002722 | NM_000516 |
| About the gene | |
| Official Symbol | GNAS |
| Previous Symbol | GNAS1 |
| Official Full Name | GNAS complex locus |
| Synonyms | NESP55; NESP; GNASXL; GPSA; SCG6; SgVI |
| Location | 20q13.32 |
| Gene Type | protein-coding gene |
| Uniprot ID | O95467|P63092|P84996|Q5JWF2 |
| Pathway/Library | Oncogenes Library |
| Gene Summary | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. |
| 产品编号 | 产品名称 | 包装 |
| L03655 | pLenti-GNAS-sgRNA | 5µg |
| — | 说明书 | 1份 |
-20℃保存，至少两年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA与质粒及其序列信息，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
慢病毒包装使用的包装质粒，可以订购碧云天的Lentivirus Packaging Vectors Set A (L00002)，包括pMDLg、Rev和VSV-g。
对于非目录产品的CRISPR基因敲除用的sgRNA表达质粒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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