产品编号 | 产品名称 | 产品包装 | 产品价格 |
P5017-10mg | Recombinant Protein A | 10mg | 379.00元 |
P5017-100mg | Recombinant Protein A | 100mg | 1598.00元 |
P5017-1g | Recombinant Protein A | 1g | 6668.00元 |
Species | Gene ID | Accession | Source | Length | Calculated MW | Tag |
- | - | - | E. coli | 295aa | ~33kDa | - |
About this protein | |
Name | Recombinant Protein A (rProtein A; Protein A);重组蛋白A |
Synonyms | N/A |
Purity | >98% by SDS-PAGE and HPLC analyses |
Biological Activity | The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from additive free solution. |
Endotoxin | Less than 0.1EU/µg of Protein A as determined by LAL method. |
Reconstitution | Dissolve in distilled water or saline. |
Category | Protein A |
Background | Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E. Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series [1-2]. |
Amino Acid Sequence | SAQHDEAQQN AFYQVLNMPN LNADQRNGFI QSLKDDPSQS ANVLGEAQKL NDSQAPKADA QQNNFNKDQQ SAFYEILNMP NLNEAQRNGF IQSLKDDPSQ STNVLGEAKK LNESQAPKAD NNFNKEQQNA FYEILNMPNL NEEQRNGFIQ SLKDDPSQSA NLLSEAKKLN ESQAPKADNK FNKEQQNAFY EILHLPNLNE EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNKFN KEQQNAFYEI LHLPNLTEEQ RNGFIQSLKD DPSVSKEILA EAKKLNDAQA PKEED |
产品编号 | 产品名称 | 包装 |
P5017-10mg | Recombinant Protein A | 10mg |
P5017-100mg | Recombinant Protein A | 10mg×10 |
P5017-1g | Recombinant Protein A | 1g |
— | 说明书 | 1份 |
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
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