产品编号 | 产品名称 | 产品包装 | 产品价格 |
P5036-10mg | Recombinant Protein G Plus | 10mg | 898.00元 |
P5036-100mg | Recombinant Protein G Plus | 100mg | 3998.00元 |
P5036-1g | Recombinant Protein G Plus | 1g | 16998.00元 |
Species | Gene ID | Accession | Source | Length | Calculated MW | Tag |
- | - | - | E. coli | - | ~40kDa | - |
About this protein | |
Name | Recombinant Protein G Plus (rProtein G Plus; Protein G Plus);重组蛋白G Plus;高载量重组蛋白G |
Synonyms | N/A |
Purity | >98% by SDS-PAGE and HPLC analyses. |
Biological Activity | The recombinant Protein G Plus is a genetically engineered protein containing 3 IgG-binding regions of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein G to ensure the maximum specific IgG binding. The recombinant Protein G is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house and rabbit. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from additive free solution. |
Endotoxin | Less than 0.1EU/μg of Protein G Plus as determined by LAL method. |
Reconstitution | Dissolve in distilled water or saline. |
Category | Protein G Plus |
Background | Protein G Plus is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G Plus binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology [1-2]. 本Recombinant Protein G Plus与传统Recombinant Protein G相比,稳定性更好,特异性结合相应抗体能力更强,蛋白相同用量情况下Recombinant Protein G Plus能多结合约30-50%的抗体。 |
Amino Acid Sequence | - |
产品编号 | 产品名称 | 包装 |
P5036-10mg | Recombinant Protein G Plus | 10mg |
P5036-100mg | Recombinant Protein G Plus | 10mg×10 |
P5036-1g | Recombinant Protein G Plus | 1g |
— | 说明书 | 1份 |
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
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