使用说明：
1. 试剂盒的准备工作：
a.GSSG储备液的配制：在本试剂盒提供的5mg GSSG中加入816微升MilliQ级纯水，溶解并混匀，即为GSSG储备液，浓度为10mM。除立即待用部分外，其余GSSG储备液适当分装后-20℃保存。
b.DTNB储备液的配制：在本试剂盒提供的4.5mg DTNB中加入1.5毫升本试剂盒提供的DMSO，溶解并混匀，即为DTNB储备液。除立即待用部分外，其余DTNB储备液适当分装后-20℃保存。
c.蛋白去除试剂M溶液的配制：称取0.2克蛋白去除试剂M，加入4毫升总谷胱甘肽检测缓冲液，配制成4毫升5%的水溶液。蛋白去除试剂M溶液必须新鲜配制并限当天使用。
d.NADPH储备液(40mg/ml)的配制：在本试剂盒提供的4mg NADPH中加入100微升MilliQ级纯水，溶解并混匀，即为NADPH储备液。除立即待用部分外，其余NADPH储备液适当分装后-70℃保存。
e.5倍稀释谷胱甘肽还原酶的配制：取50微升谷胱甘肽还原酶，加入200微升总谷胱甘肽检测缓冲液，混匀，即成5倍稀释的谷胱甘肽还原酶。
f.总谷胱甘肽检测工作液的配制：根据待检测的样品数参考下表配制适当量的总谷胱甘肽检测工作液，表中三种试剂按比例混合后即为总谷胱甘肽检测工作液。

g.0.5mg/ml NADPH的配制：取10微升NADPH储备液，加入790微升总谷胱甘肽检测缓冲液，混匀即为0.5mg/ml NADPH。每检测一个样品需50微升0.5mg/ml NADPH。
h.稀释的GSH清除辅助液的配制：在47微升MilliQ级纯水中加入53微升GSH清除辅助液，立即混匀。稀释后的GSH清除辅助液不太稳定，每次使用时均须新鲜配制，并仅限当日使用。
i.GSH清除试剂工作液的配制：10.8微升GSH清除试剂中加入89.2微升无水乙醇，立即混匀。GSH清除剂工作液每次也须新鲜配制。
2. 标准品的准备：
a.把10mM GSSG储备液用蛋白去除试剂M溶液稀释成15μM GSSG溶液。然后依次稀释成10、5、2、1、0.5μM GSSG溶液。取15、10、5、2、1、0.5μM GSSG溶液六个点做标准曲线。注意：由于GSSG在蛋白去除试剂M溶液中不太稳定，用蛋白去除试剂M溶液配制的GSSG溶液必须新鲜配制后使用，不可冻存后再使用。
b.如果需要测定样品中GSSG含量，按照每100微升标准品加入5微升稀释的GSH清除辅助液的比例加入稀释的GSH清除辅助液，立即vortex混匀。然后按照每100微升加入1微升GSH清除试剂工作液的比例加入GSH清除试剂工作液，立即vortex混匀，25℃反应60分钟。即可用于后续的GSSG含量的检测。
3. 待测总谷胱甘肽含量样品和标准品的准备：
a.组织样品的准备。取组织用液氮速冻，然后研成粉末。每10毫克研碎的组织粉末，加入30微升蛋白去除试剂M溶液，充分Vortex。再加入70微升蛋白去除试剂M溶液，用玻璃匀浆器充分匀浆(对于比较容易匀浆的组织可以不用液氮速冻等处理，而直接加入适量蛋白去除试剂M溶液进行匀浆)。4℃放置10分钟后，10,000g 4℃离心10分钟，取上清用于总谷胱甘肽的测定。样品需暂时4℃保存，不立即测定的样品可以-70℃保存，但不宜超过10天。对于处理好的组织样品通常需用蛋白去除试剂M溶液进行适当稀释后再进行测定，稀释倍数通常为5-20倍。
b.细胞样品的准备。请尽量使用新鲜的细胞进行测定，而不要使用冻存的细胞进行测定。PBS洗涤细胞一次，离心收集细胞，吸尽上清。加入细胞沉淀体积3倍量的蛋白去除试剂M溶液，即如果细胞沉淀为10微升，则加入30微升蛋白去除试剂M溶液，充分Vortex。(细胞沉淀的体积可以根据细胞沉淀的重量进行估算。收集细胞前后分别对离心管进行称重，从而就可以计算出细胞沉淀的重量。10毫克细胞沉淀的体积可以粗略地看做10微升。) 然后利用液氮和37℃水浴对样品进行两次快速的冻融。4℃或冰浴放置5分钟。4℃，10,000g离心10分钟。取上清用于总谷胱甘肽的测定。样品需暂时4℃保存，不立即测定的样品可以-70℃保存，但不宜超过10天。对于处理好的细胞样品通常需用蛋白去除试剂M溶液进行适当稀释后再进行测定，稀释倍数可以高达20倍。
c.红细胞或血浆样品的准备。请尽量使用新鲜的血液进行测定。600g离心10分钟，沉淀为红细胞，上清为血浆。对于红细胞，用PBS洗涤两次。取约50微升红细胞沉淀或血浆，加入50微升蛋白去除试剂M溶液，充分Vortex。4℃或冰浴放置10分钟。4℃，10,000g离心10分钟。取上清用于总谷胱甘肽的测定。样品需暂时4℃保存，不立即测定的样品可以-70℃保存，但不宜超过10天。对于处理好的红细胞样品最后需用蛋白去除试剂M溶液稀释10倍后再进行后续的测定，而对于血浆样品，应直接取10微升进行测定。
d.说明：对于一些谷胱甘肽含量特别低的样品，可以通过冷冻干燥进行浓缩后再进行测定。
4. 待测GSSG含量样品的准备：
取部分上述准备好的待测总谷胱甘肽含量的样品，按照每100微升样品加入5微升稀释的GSH清除辅助液的比例加入稀释的GSH清除辅助液，立即vortex混匀。再按照每100微升样品加入1微升GSH清除试剂工作液的比例加入GSH清除工作液，立即vortex混匀，25℃反应60分钟。通过上述反应可以清除高达1mM的GSH，如果样品中GSH含量过高需进行适当稀释后再进行去除GSH的操作。通过上述处理就可以用于后续的测定。
5. 样品和标准品的测定：
a.参考下表，使用96孔板，依次加入样品或标准品，混匀。加入150微升总谷胱甘肽检测工作液后，混匀，25℃或室温孵育5分钟。

b.加入50微升0.5mg/ml NADPH溶液，混匀。
c.立即用酶标仪测定A₄₁₂，每5分钟测定一次或实时测定，共测定25分钟，测得5个数据。(说明：为了简化实验步骤，可以在加入NADPH溶液混匀后25分钟，仅测定一次A₄₁₂)。如果仪器可以设置温度，把温度设置在25℃，否则就在室温状况下测定。如果酶标仪不能测定A₄₁₂，可以测定405-414nm附近范围的吸光度。如果标准曲线良好，但样品的吸光度比较低，可以延长孵育时间至30-60分钟，标准品和样品的吸光度在一定范围内会随时间的延长接近于线性增加的。
注意：如果进行GSSG含量测定，标准品也须平行地进行去除GSH的相关操作，以减小误差。如果样品需同时测定总谷胱甘肽含量和GSSG含量，由于两者的检测体系不同，须分别单独做标准曲线。
d.标准品的实测效果参考图1。

图1. GSH标准曲线实测效果图。图中数据仅供参考，实际的检测效果可能会因具体反应条件的不同而有所不同。
6. 样品中总谷胱甘肽含量的计算：
a.单点测定法：即反应25分钟(或30-60分钟)后仅测定一次吸光度。根据不同浓度标准品测得的不同吸光度作出标准曲线。样品对照标准曲线即可计算出总谷胱甘肽或GSSG的含量。实际计算出来的总谷胱甘肽的含量相当于把氧化型谷胱甘肽的含量乘以2再加上还原型谷胱甘肽的含量。单点法测定相对比较便捷，而动力学法测定则相对比较精确。注意：GSSG的浓度如果换算成GSH的浓度需乘以2，例如GSSG的浓度为5μM，则相当于GSH的浓度为10μM。
b.动力学测定法：先根据不同时间点测定得到的吸光度值计算出ΔA₄₁₂/min。然后以标准品的浓度为横坐标，以ΔA₄₁₂/min为纵坐标，做出标准曲线。根据样品的ΔA₄₁₂/min，对照标准曲线就可以计算出测定时样品中总谷胱甘肽或GSSG的含量。
c.同时根据样品的稀释倍数以及最初样品的使用量，可以计算出每毫克组织或细胞中的总谷胱甘肽或GSSG的含量。对于细胞样品，也可以根据最初细胞的使用数量，然后另外取一定数量的细胞裂解后测定蛋白浓度，从而计算出细胞样品的蛋白量，最后计算出每毫克蛋白中总谷胱甘肽或GSSG的含量。
d.根据测定得到的总谷胱甘肽的含量和GSSG的含量就可以计算出GSH的含量。例如总谷胱甘肽的浓度是15μM (GSH)，GSSG的浓度是1.2μM，那么GSH的浓度为15-1.2X2=12.6μM。

使用本产品的相关论文：
1. Jia JH, Wang Y, Cao YB, Gao PH, Jia XM, Ma ZP, Xu YG, Dai BD, Jiang YY.
   CaIPF7817 is involved in the regulation of redox homeostasis in Candida albicans.
   Biochem Biophys Res Commun. 2007 Jul 20;359(1):163-7.
2. Wang B, Feng WY, Zhu MT, Wang Y, Wang M, Gu YQ, Ouyang H, Wang HJ, Li M, Zhao YL, Chai
   ZF, Wang HF.
   Neurotoxicity of low-dose repeatedly intranasal instillation of nano- and submicron-
   sized ferric oxide particles in mice.
   J Nanopart Res. 2007 Sep;118(3):233-43.
3. Ji LL, Chen Y, Wang ZT.
   Yamaoka Ligularia base of HEK293 cells glutathione content and the impact of related
   activity.
   J Toxicol June.2007 Vol.21 No.3.
4. Zhu MT, Feng WY, Wang B, Wang TC, Gu YQ, Wang M, Wang Y, Ouyang H, Zhao YL, Chai ZF.
   Comparative study of pulmonary responses to nano- and submicron-sized ferric oxide in
   rats.
   Toxicology. 2008 May 21;247(2-3):102-11.
5. Bi J, Jiang B, Liu JH, Zhang XL, An LJ.
   Protective effects of catalpol against H(2)O(2)-induced oxidative stress in astrocytes
   primary cultures.
   Neurosci Lett. 2008 Sep 19;442(3):224-7.
6. Fan J, Cai H, Yang S, Yan L, Tan W.
   Comparison between the effects of normoxia and hypoxia on antioxidant enzymes and
   glutathione redox state in ex vivo culture of CD34(+) cells.
   Comp Biochem Physiol B Biochem Mol Biol. 2008 Oct;151(2):153-8.
7. Wang L, Yan B, Liu N, Li Y, Wang Q.
   Effects of cadmium on glutathione synthesis in hepatopancreas of freshwater crab,
   Sinopotamon yangtsekiense.
   Chemosphere. 2008 Dec;74(1):51-6.
8. Zhang P, Xua Y, Li L, Jiang Q, Wang M, Jin L.
   In vitro protective effects of pyrroloquinoline quinone on methylmercury-induced
   neurotoxicity.
   Environmental Toxicology and Pharmacology 27 (2009) 103–110.
9. Liu Z, Zhang X, Bai J, Suo B, Xu P, Wang L.
   Exogenous paraquat changes antioxidant enzyme activities and lipid peroxidation in
   drought-stressed cucumber leaves.
   Scientia Horticulturae 121 (2009) 138–143.
10.Dong HS, Li L, Song ZH, Tang J, Xu B, Zhai XW, Sun LL, Zhang P, Li ZB, Pan QJ, Shi QH,
   Shen W.
   Premeiotic fetal murine germ cells cultured in vitro form typical oocyte-like cells but
   do not progress through meiosis.
   Theriogenology. 2009 Jul 15;72(2):219-31. Epub 2009 Apr 9.
11.LIU JJ, LIN SH, XU PL, WANG XJ, BAI JG.
   Effects of Exogenous Silicon on the Activities of Antioxidant Enzymes and Lipid
   Peroxidation in Chilling-Stressed Cucumber Leaves.
   Agricultural Sciences in China. 2009, 8(9): 1075-1086.
12.Sun ZW, Zhang L, Zhu SJ, Chen WC, Mei B.
   Excitotoxicity effects of glutamate on human neuroblastoma SH-SY5Y cells via oxidative
   damage.
   Neurosci Bull. 2010;26(1):8-16.
13.Xu JY, Su YY, Cheng JS, Li SX, Liu RL, Li WX, Xu GT, Li QN.
   Protective effects of fullerenol on carbon tetrachloride-induced acute hepatotoxicity
   and nephrotoxicity in rats.
   Sciencedirect Carbon. 48(2010)1388–1396.
14.Cao X, Xiao H, Zhang Y, Zou L, Chu Y, Chu X.
   1, 5-Dicaffeoylquinic acid-mediated glutathione synthesis through activation of Nrf2
   protects against OGD/reperfusion-induced oxidative stress in astrocytes.
   Brain Res. 2010;1347:142-8. Epub 2010 Jun 1.
15.Zhang J, Zhao QZ, Duan GL, Huang YC.
   Influence ofsulphuronarsenicaccumulationandmetabolisminriceseedlings.
   Environmental andExperimentalBotany.(2010).
16.Sun L, Xu S, Zhou M, Wang C, Wu Y, Chan P.
   Effects of cysteamine on MPTP-induced dopaminergic neurodegeneration in mice.
   Brain Res. 2010;1335:74-82. Epub 2010 Apr 7.
17.Huang JZ, Chen YZ, Su M, Zheng HF, Yang YP, Chen J, Liu CF.
   dl-3-n-Butylphthalide prevents oxidative damage and reduces mitochondrial dysfunction in
   an MPP(+)-induced cellular model of Parkinson's disease.
   Neurosci Lett. 2010;475(2):89-94. Epub 2010 Mar 27.
18.Liu ZJ, Guo YK, Bai JG.
   Exogenous hydrogen peroxide changes antioxidant enzyme activity and protects
   ultrastructure in leaves of two cucumber ecotypes under osmotic stress.
   JOURNAL OF PLANT GROWTH REGULATION,2010,29(7),171-183.
19.Yang CR, Miao DQ, Zhang QH, Guo L, Tong JS, Wei Y, Huang X, Hou Y, Schatten H, Liu Z,
   Sun QY.
   Short-term preservation of porcine oocytes in ambient temperature: novel approaches.
   PLoS One. 2010 Dec 7;5(12):e14242.
20.Wang W, Feng MF, Lin Z.
   The Glutathione and Metallothionein Contents Variation in Earthworm Exposed by AHTN.
   Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th ,1-4.
21.Liu J, Zhou J, An W, Lin Y, Yang Y, Zang W.
   Apocynin attenuates pressure overload-induced cardiac hypertrophy in rats by reducing
   levels ofreactive oxygen species.
   Can J Physiol Pharmacol. 2010 Jul;88(7):745-52.
22.Ding B, Yuan L, Yu H, Li L, Ma W, Bi Y, Feng J, Xiao R.
   Genistein and folic acid prevent oxidative injury induced by β-amyloid peptide.
   Basic Clin Pharmacol Toxicol. 2011 May;108(5):333-40.
23.Zhang XL,Jia XF, Yu B, Gao Y, Bai JG.
   Exogenous hydrogen peroxide influences antioxidant enzyme activity and lipid
   peroxidation in cucumber leaves at low light.
   Scientia Horticulturae,2011Jul,129(4),656-662.
24.Li Q, Yu B, Gao Y, Dai AH, Bai JG.
   Cinnamic acid pretreatment mitigates chilling stress of cucumber leaves through
   alteringantioxidant enzyme activity.
   J Plant Physiol. 2011 Jun 15;168(9):927-34.
25.Hu L, Chen L, Yang G, Li L, Sun H, Chang Y, Tu Q, Wu M, Wang H.
   HBx sensitizes cells to oxidative stress-induced apoptosis by accelerating the loss of
   Mcl-1 proteinvia caspase-3 cascade.
   Mol Cancer. 2011 Apr 20;10:43.
26.Sun WJ, Nie YX, Gao Y, Dai AH, Bai JG.
   Exogenous cinnamic acid regulates antioxidant enzyme activity and reduces lipid
   peroxidation in drought-stressed cucumber leaves.
   ACTA PHYSIOLOGIAE PLANTARUM,2012,34(2),641-655.
27.Jia P, Xu YJ, Zhang ZL, Li K, Li B, Zhang W, Yang H.
   Ferric ion could facilitate osteoclast differentiation and bone resorption through the
   production ofreactive oxygen species.
   J Orthop Res. 2012 May 8.
28.Wang J, Hou Y, Dong L, Niu X, Fan Y.
   Influence of TiO2 nanoparticles on glutathione in rat synovial cell line RSC-364.
   World Congress on Medical Physics and Biomedical Engineering. May 26-31, 2012,
   Beijing, China.
29.Zhong WX, Wang YB, Peng L, Ge XZ, Zhang J, Liu SS, Zhang XN, Xu ZH, Chen Z, Luo JH.
   Lanthionine synthetase C-like protein 1 interacts with and inhibits cystathionine
   β-synthase: a target for neuronalantioxidant defense.
   J Biol Chem. 2012 Oct 5;287(41):34189-201. doi: 10.1074/jbc.M112.383646.
   Epub 2012 Aug 13.
30.Zhang B, Chen N, Chen H, Wang Z, Zheng Q.
   The critical role of redox homeostasis in shikonin-induced HL-60 cell differentiation
   via unique modulation of theNrf2/ARE pathway.
   Oxid Med Cell Longev. 2012;2012:781516. doi: 10.1155/2012/781516. Epub 2012 Oct 15.
31.Zhang J, Li D, Sun W, Wang X, Bai J.
   Exogenous p-hydroxybenzoic acid regulates antioxidant enzyme activity and mitigates heat
   stress of cucumber leaves.
   Scientia Horticulturae.Volume 148, 4 December 2012, Pages 235–245.
32.Dai A, Nie Y, Yu B, Li Q, Lu L,Bai J.
   Cinnamic acid pretreatment enhances heat tolerance of cucumber leaves through modulating
   antioxidant enzyme activity.
   Environmental and Experimental Botany.Volume 79, July 2012, Pages 1–10.
33.Zhang L, Zhang J, Zhao B, Zhao-Wilson X.
   Quinic acid could be a potential rejuvenating natural compound by improving survival
   of Caenorhabditis elegansunder deleterious conditions.
   Rejuvenation Res. 2012 Dec;15(6):573-83. doi: 10.1089/rej.2012.1342.
   Epub 2012 Sep 24.
34.Liu L, Wang P, Liang C, He D, Yu Y, Liu X.
   Distinct effects of Nampt inhibition on mild and severe models of
   lipopolysaccharide-induced myocardialimpairment.
   Int Immunopharmacol. 2013 Jul 2. pii: S1567-5769(13)00258-0.
35.Hou Y, Lai M, Chen X, Li J, Hu Y, Luo Z, Ding X, Cai K.
   Effects of mesoporous SiO2 , Fe3 O4 , and TiO2 nanoparticles on the biological functions
   of endothelial cells in vitro.
   J Biomed Mater Res A. 2013 Jun 15. doi: 10.1002/jbm.a.34839.
36.Ma WW, Hou CC, Zhou X, Yu HL, Xi YD, Ding J, Zhao X, Xiao R.
   Genistein alleviates the mitochondria-targeted DNA damage induced by β-amyloid peptides
   25-35 in C6 gliomacells.
   Neurochem Res. 2013 Jul;38(7):1315-23. doi: 10.1007/s11064-013-1019-y.
   Epub 2013 Mar 22.
37.Wang J, Jiang Z, Ji J, Wang X, Wang T, Zhang Y, Tai T, Chen M, Sun L, Li X, Zhang L.
   Gene expression profiling and pathway analysis of hepatotoxicity induced by triptolide
   in Wistar rats.
   Food Chem Toxicol. 2013 Apr 29;58C:495-505. doi: 10.1016/j.fct.2013.04.039.
38.Ren C, Li Y, Han R, Gao D, Li W, Shi J, Hoogewijs D, Braeckman BP, De Henau S, Lu Y,
   Qu W, Gao Y, Wu Y, Li Z, Liu H, Wang Z, Zhang C.
   GLB-13 is associated with oxidative stress resistance in caenorhabditis elegans.
   IUBMB Life. 2013 May;65(5):423-34. doi: 10.1002/iub.1132. Epub 2013 Mar 18.
39.Dai BD, Wang Y, Zhao LX, Li DD, Li MB, Cao YB, Jiang YY.
   Cap1p attenuates the apoptosis of Candida albicans.
   FEBS J. 2013 Jun;280(11):2633-43. doi: 10.1111/febs.12251. Epub 2013 Apr 8.
40.Khan M, Li T, Ahmad Khan MK, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T.
   Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of
   STAT3 activation, andmitochondrial dysfunction.
   Biomed Res Int. 2013;2013:719858. doi: 10.1155/2013/719858. Epub 2012 Dec 27.
41.Jiang Q, Zhou Z, Wang L, Shi X, Wang J, Yue F, Yi Q, Yang C, Song L.
   The immunomodulation of inducible nitric oxide in scallop Chlamys farreri.
   Fish Shellfish Immunol. 2013 Jan;34(1):100-8. doi: 10.1016/j.fsi.2012.10.011.
   Epub 2012 Oct 13.
42.Zhang L, Wang L, Hu Y, Liu Z, Tian Y, Wu X, Zhao Y, Tang H, Chen C, Wang Y.
   Selective metabolic effects of gold nanorods on normal and cancer cells and their
   application in anticancer drugscreening.
   Biomaterials. 2013 Sep;34(29):7117-26. doi: 10.1016/j.biomaterials.2013.05.043.
   Epub 2013 Jun 18.
43.Jing C, Li X, Zhang J, Wang J.
   Responses of the Antioxidant System in QGY-7701 Cells to the Cytotoxicity and Apoptosis
   Induced by 1-Octyl-3-methylimidazolium Chloride.
   J Biochem Mol Toxicol. 2013 Jun;27(6):330-6. doi: 10.1002/jbt.21495.
   Epub 2013 May 20.
44.Wang C, Chen H, Zhang J, Hong Y, Ding X, Ying W.
   Malate-aspartate shuttle mediates the intracellular ATP levels, antioxidation capacity
   and survival of differentiated PC12 cells.
   Int J Physiol Pathophysiol Pharmacol. 2014 Jul 12;6(2):109-14. eCollection 2014.
45.Meng G, Xia M, Xu C, Yuan D, Schnurr M, Wei J.
   Multifunctional antitumor molecule 5'-triphosphate siRNA combining glutaminase silencing
   and RIG-I activation.
   Int J Cancer. 2014 Apr 15;134(8):1958-71. doi: 10.1002/ijc.28416. Epub 2013 Dec 2.
46.Mo C, Wang L, Zhang J, Numazawa S, Tang H, Tang X, Han X, Li J, Yang M, Wang Z, Wei D,
   Xiao H.
   The crosstalk between Nrf2 and AMPK signal pathways is important for the
   anti-inflammatory effect of berberine in LPS-stimulated macrophages and endotoxin-
   shocked mice.
   Antioxid Redox Signal. 2014 Feb 1;20(4):574-88. doi: 10.1089/ars.2012.5116. Epub
   2013 Sep 17.
47.Xie JM, Li B, Yu HP, Gao QG, Li W, Wu HR, Qin ZH.
   TIGAR has a dual role in cancer cell survival through regulating apoptosis and
   autophagy.
   Cancer Res. 2014 Sep 15;74(18):5127-38. doi: 10.1158/0008-5472.CAN-13-3517. Epub 2014
   Aug 1.
48.Shi H, Ye T, Han N, Bian H, Liu X, Chan Z.
   Hydrogen sulfide regulates abiotic stress tolerance and biotic stress resistance in
   Arabidopsis.
   J Integr Plant Biol. 2014 Oct 20. doi: 10.1111/jipb.12302.
49.He X, Song W, Liu C, Chen S, Hua J.
   Rapamycin inhibits acrolein-induced apoptosis by alleviating ROS-driven mitochondrial
   dysfunction in male germ cells.
   Cell Prolif. 2014 Apr;47(2):161-71. doi: 10.1111/cpr.12091. Epub 2014 Jan 31.
50.Shi H, Jiang C, Ye T, Tan D, Reiter RJ, Zhang H, Liu R, Chan Z.
   Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of
   improved abiotic stress resistance in bermudagrass [Cynodon dactylon (L). Pers.] by
   exogenous melatonin.
   J Exp Bot. 2014 Sep 15. pii: eru373.
51.Wang Y, Zhao W, Hao J, Xu W, Luo Y, Wu W, Yang Z, Liang Z, Huang K.
   Changes in biosynthesis and metabolism of glutathione upon ochratoxin A stress in
   Arabidopsis thaliana.
   Plant Physiol Biochem. 2014 Jun;79:10-8. doi: 10.1016/j.plaphy.2014.03.001. Epub
   2014 Mar 12.
52.Zhang B, Wang XQ, Chen HY, Liu BH.
   Involvement of the Nrf2 Pathway in the Regulation of Pterostilbene-Induced Apoptosis in
   HeLa Cells via ER Stress.
   J Pharmacol Sci. 2014 Nov 20;126(3):216-229. Epub 2014 Oct 21.
53.Diao Y, Xu H, Li G, Yu A, Yu X, Hu W, Zheng X, Li S, Wang Y, Hu Z.
   Cloning a glutathione peroxidase gene from Nelumbo nucifera and enhanced salt tolerance
   by overexpressing in rice.
   Mol Biol Rep. 2014 Aug;41(8):4919-27. doi: 10.1007/s11033-014-3358-4. Epub 2014 Apr 9.
54.Xu W, Wang S, Chen Q, Zhang Y, Ni P, Wu X, Zhang J, Qiang F, Li A, Røe OD, Xu S, Wang M,
   Zhang R, Zhou J.
   TXNL1-XRCC1 pathway regulates cisplatin-induced cell death and contributes to resistance
   in human gastric cancer.
   Cell Death Dis. 2014 Feb 13;5:e1055. doi: 10.1038/cddis.2014.27.
55.Zhou H, Wang X, Zhou Y, Yao H, Ahmad F.
   Evaluation of the toxicity of ZnO nanoparticles to Chlorella vulgaris by use of the
   chiral perturbation approach.
   Anal Bioanal Chem. 2014 Jun;406(15):3689-95. doi: 10.1007/s00216-014-7773-0. Epub
   2014 Apr 22.