Fura-2 AM(钙离子荧光探针)

 产品编号: S1052
 产品包装: 50微升
 产品价格: 289.00元
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产品简介

产品简介:

产品编号 产品名称 产品包装 产品价格
S1052 Fura-2 AM(钙离子荧光探针) 50微升 289.00元

    Fura-2 AM,也称Fura-2 pentakis(acetoxymethyl)ester,是最常用的检测细胞内钙离子浓度的荧光探针之一。分子式为C44H47N3O24,分子量为1001.9。
    本Fura-2 AM(钙离子荧光探针)是配制于无水DMSO(anhydrous DMSO)中的储存液,浓度为2mM。
    Fura-2 AM是一种可以穿透细胞膜的荧光染料。Fura-2 AM的荧光比较弱,最大激发波长为369nm,最大发射波长为478nm,并且其荧光不会随钙离子浓度改变而改变。Fura-2 AM进入细胞后可以被细胞内的酯酶剪切形成Fura-2,从而被滞留在细胞内。Fura-2可以和钙离子结合,结合钙离子后在330-350nm激发光下可以产生较强的荧光,而在380nm激发光下则会导致荧光减弱。这样就可以使用340nm和380nm这两个荧光的比值来检测细胞内的钙离子浓度,可以消除不同细胞样品间荧光探针装载效率的差异,荧光探针的渗漏,细胞厚度差异等一些误差因素。Fura-2和钙离子结合后,最大激发波长为335nm(最大激发波长随离子浓度的的不同而有所不同),最大发射波长为505nm。实际检测时推荐使用的激发波长为340nm,发射波长为510nm。如果做双波长检测,则推荐使用的激发波长为340nm和380nm。Fura-2的激发光谱参考下图,不同曲线表示不同钙离子浓度时的激发光谱。
                    
    Fura-2相对而言有较强的抗荧光淬灭能力,在荧光显微镜或其它荧光检测设备上可以连续检测1小时而不明显影响其荧光效果。
    用于细胞内钙离子检测时,Fura-2 AM的常用浓度为0.5-5μM。通常用含有0.5-5μM的Fura-2 AM的适当溶液和细胞一起在4-37℃孵育15-60分钟,即可完成荧光探针的装载。
包装清单:

产品编号

产品名称

包装

S1052

Fura-2 AM(钙离子荧光探针, 2mM)

50微升

说明书

1份

保存条件:
    -20℃避光保存,6个月有效。
注意事项:
    本Fura-2 AM在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内,可以20-25℃水浴温育片刻至全部融解后使用。
    荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。
    为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明

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