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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| L00013-5μg | pLenti-GFP-sgRNA | 5μg | 999.00元 |
| L00013-100μg | pLenti-GFP-sgRNA | 100μg | 1129.00元 |
| L00017 | GFP Knockout Lentivirus | 10^8 TU | 1999.00元 |
| L00022 | GFP Knockout HEK293T Cells | 1支/瓶 | 1299.00元 |
| L00027 | GFP Knockout HEK293T RIPA Lysate | 100μg | 999.00元 |
| L00033 | GFP Knockout HEK293T Trizol Lysate | 500μl | 999.00元 |
GFP Knockout Lentivirus (GFP基因敲除对照慢病毒)是一种感染动物细胞后可以同时表达Cas9、GFP sgRNA和puromycin抗性基因的慢病毒。本产品用于在动物细胞中基于CRISPR/Cas9技术敲除GFP的病毒。本对照病毒中的sgRNA靶向GFP。本病毒或Control Knockout Lentivirus (L00015)是CRISPR/Cas9技术敲除目的基因的常用对照病毒。
本慢病毒基因序列的关键图谱信息请参考图1。本慢病毒可用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除的对照。
图1.可同时表达sgRNA、Cas9和puromycin抗性的本慢病毒其基因序列的关键图谱信息。注：本对照病毒中的sgRNA靶向GFP。
用于包装本慢病毒的质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
碧云天同时提供基于CRISPR/Cas9技术的GFP基因敲除的质粒(L00013 pLenti-GFP-sgRNA)、慢病毒(L00017 GFP Knockout Lentivirus)、HEK293T细胞(L00022 GFP Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L00027 GFP Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L00033 GFP Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
GFP基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Aequorea victoria | GFP | 7011691 | P42212 | X96418 |
| About the gene | |
| Official Symbol | GFP |
| Previous Symbol | - |
| Official Full Name | Green fluorescent protein |
| Synonyms | - |
| Location | - |
| Gene Type | protein-coding gene |
| Uniprot ID | P42212 |
| Pathway/Library | - |
| Gene Summary | Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9kDa protein (composed of 238a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37℃, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids. |
| 产品编号 | 产品名称 | 包装 |
| L00017 | GFP Knockout Lentivirus | 108 TU |
| — | 说明书 | 1份 |
-80℃保存，至少一年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA序列信息与本慢病毒，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
对于非目录产品的CRISPR基因敲除用的慢病毒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| L00015 | Control Knockout Lentivirus | 108 TU |
| L00017 | GFP Knockout Lentivirus | 108 TU |
| C0222 | 青霉素-链霉素溶液(100X) | 100ml |
| C0351-1ml | Polybrene (Hexadimethrine Bromide) | 1ml |
| C0351-50mg | Polybrene (Hexadimethrine Bromide) | 50mg |
| D0508S/M | 基因组编辑突变检测试剂盒 | 25/100次 |
| D7080S/M/L | T7 Endonuclease I (CRISPR等基因突变鉴定用) | 250/1250/5000U |
| ST551-10mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×1ml |
| ST551-50mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×5ml |
| ST551-250mg | Puromycin Dihydrochloride (嘌呤霉素) | 250mg |
| ST1380-500mg | Polybrene (≥94%, Reagent grade) | 500mg |
| ST1380-2g | Polybrene (≥94%, Reagent grade) | 2g |
| ST1380-10g | Polybrene (≥94%, Reagent grade) | 10g |
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