产品编号 | 产品名称 | 产品包装 | 产品价格 |
L05400 | DPF1基因敲除质粒 | 5µg | 999.00元 |
L05401 | DPF1基因敲除慢病毒 | 10^8 TU | 6999.00元 |
L05402 | DPF1基因敲除HEK293T细胞 | 1支/瓶 | 7999.00元 |
L05403 | DPF1基因敲除HEK293T细胞RIPA裂解液 | 100µg | 1258.00元 |
L05404 | DPF1基因敲除HEK293T细胞Trizol裂解液 | 500µl | 1258.00元 |
DPF1 Knockout Lentivirus(DPF1基因敲除慢病毒)是一种感染动物细胞后可以同时表达Cas9、目的基因sgRNA和puromycin抗性基因的慢病毒。本产品用于在动物细胞中基于CRISPR/Cas9技术敲除目的基因,并且本慢病毒中sgRNA的有效性已经通过T7EI法的验证。
本慢病毒基因序列的关键图谱信息请参考图1。本慢病毒可用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 可同时表达sgRNA、Cas9和puromycin抗性的本慢病毒其基因序列的关键图谱信息。
用于包装本慢病毒的质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得,sgRNA的有效性已经通过T7EI法验证。
本慢病毒用于实验时,建议同时选购无任何靶向的对照慢病毒Control Knockout Lentivirus (L00015)或靶向GFP的对照慢病毒GFP Knockout Lentivirus (L00017)。
碧云天同时提供基于CRISPR/Cas9技术的DPF1基因敲除的质粒(L05400 pLenti-DPF1-sgRNA)、慢病毒(L05401 DPF1 Knockout Lentivirus)、HEK293T细胞(L05402 DPF1 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L05403 DPF1 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L05404 DPF1 Knockout HEK293T Trizol Lysate)等产品,具体请在碧云天网站查询或在本产品网页点击相应产品。
DPF1基因的基本信息如下:
Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
Human | DPF1 | 8193 | BC125153 | NM_001135155 |
About the gene | |
Official Symbol | DPF1 |
Previous Symbol | - |
Official Full Name | double PHD fingers 1 |
Synonyms | neuro-d4; NEUD4; BAF45b |
Location | 19q13.2 |
Gene Type | protein-coding gene |
Uniprot ID | Q92782 |
Pathway/Library | Epigenetic Regulators Related Genes Library; Ubiquitin Ligases Genes Library |
Gene Summary | May have an important role in developing neurons by participating in regulation of cell survival, possibly as a neurospecific transcription factor. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). DPF1_HUMAN,Q92782 |
产品编号 | 产品名称 | 包装 |
L05401 | DPF1 Knockout Lentivirus | 108 TU |
— | 说明书 | 1份 |
-80℃保存,至少一年有效。
注意事项:碧云天拥有sgRNA序列的知识产权,如果需要sgRNA序列,请在订购后发送邮件向info@beyotime.com索取。sgRNA序列信息与本慢病毒,未经碧云天书面许可不得用于任何商业用途,也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时,应注明来源。
对于非目录产品的CRISPR基因敲除用的慢病毒的定制,可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
产品编号 | 产品名称 | 包装 |
L00015 | Control Knockout Lentivirus | 108 TU |
L00017 | GFP Knockout Lentivirus | 108 TU |
C0222 | 青霉素-链霉素溶液(100X) | 100ml |
C0351-1ml | Polybrene (Hexadimethrine Bromide) | 1ml |
C0351-50mg | Polybrene (Hexadimethrine Bromide) | 50mg |
D0508S/M | 基因组编辑突变检测试剂盒 | 25/100次 |
D7080S/M/L | T7 Endonuclease I (CRISPR等基因突变鉴定用) | 250/1250/5000U |
ST551-10mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×1ml |
ST551-50mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×5ml |
ST551-250mg | Puromycin Dihydrochloride (嘌呤霉素) | 250mg |
ST1380-500mg | Polybrene (≥94%, Reagent grade) | 500mg |
ST1380-2g | Polybrene (≥94%, Reagent grade) | 2g |
ST1380-10g | Polybrene (≥94%, Reagent grade) | 10g |