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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| L08635 | ARIH1基因敲除质粒 | 5µg | 999.00元 |
| L08636 | ARIH1基因敲除慢病毒 | 10^8 TU | 6999.00元 |
| L08637 | ARIH1基因敲除HEK293T细胞 | 1支/瓶 | 7999.00元 |
| L08638 | ARIH1基因敲除HEK293T细胞RIPA裂解液 | 100µg | 1258.00元 |
| L08639 | ARIH1基因敲除HEK293T细胞Trizol裂解液 | 500µl | 1258.00元 |
ARIH1 Knockout Lentivirus(ARIH1基因敲除慢病毒)是一种感染动物细胞后可以同时表达Cas9、目的基因sgRNA和puromycin抗性基因的慢病毒。本产品用于在动物细胞中基于CRISPR/Cas9技术敲除目的基因，并且本慢病毒中sgRNA的有效性已经通过T7EI法的验证。
本慢病毒基因序列的关键图谱信息请参考图1。本慢病毒可用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 可同时表达sgRNA、Cas9和puromycin抗性的本慢病毒其基因序列的关键图谱信息。
用于包装本慢病毒的质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
本慢病毒用于实验时，建议同时选购无任何靶向的对照慢病毒Control Knockout Lentivirus (L00015)或靶向GFP的对照慢病毒GFP Knockout Lentivirus (L00017)。
碧云天同时提供基于CRISPR/Cas9技术的ARIH1基因敲除的质粒(L08635 pLenti-ARIH1-sgRNA)、慢病毒(L08636 ARIH1 Knockout Lentivirus)、HEK293T细胞(L08637 ARIH1 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L08638 ARIH1 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L08639 ARIH1 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
ARIH1基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | ARIH1 | 25820 | BC051877 | NM_005744 |
| About the gene | |
| Official Symbol | ARIH1 |
| Previous Symbol | - |
| Official Full Name | ariadne RBR E3 ubiquitin protein ligase 1 |
| Synonyms | HARI; HHARI; UBCH7BP; ARI |
| Location | 15q24.1 |
| Gene Type | protein_coding |
| Uniprot ID | Q9Y4X5 |
| Pathway/Library | Ubiquitin Ligases Genes Library |
| Gene Summary | E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:15236971, PubMed:21532592, PubMed:24076655, PubMed:27565346, PubMed:23707686). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets (PubMed:27565346). The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2 (PubMed:27565346). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes (PubMed:24076655, PubMed:27565346). Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear (PubMed:25624349). According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349). According to another report EIF4E2 ubiquitination leads to its subsequent degradation (PubMed:14623119). Acts as the ligase involved in ISGylation of EIF4E2 (PubMed:17289916). In vitro, controls the degradation of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex member SUN2 and may therefore have a role in the formation and localization of the LINC complex, and as a consequence, nuclear subcellular localization and nuclear morphology (PubMed:29689197). ARI1_HUMAN,Q9Y4X5 |
| 产品编号 | 产品名称 | 包装 |
| L08636 | ARIH1 Knockout Lentivirus | 108 TU |
| — | 说明书 | 1份 |
-80℃保存，至少一年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA序列信息与本慢病毒，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
对于非目录产品的CRISPR基因敲除用的慢病毒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| L00015 | Control Knockout Lentivirus | 108 TU |
| L00017 | GFP Knockout Lentivirus | 108 TU |
| C0222 | 青霉素-链霉素溶液(100X) | 100ml |
| C0351-1ml | Polybrene (Hexadimethrine Bromide) | 1ml |
| C0351-50mg | Polybrene (Hexadimethrine Bromide) | 50mg |
| D0508S/M | 基因组编辑突变检测试剂盒 | 25/100次 |
| D7080S/M/L | T7 Endonuclease I (CRISPR等基因突变鉴定用) | 250/1250/5000U |
| ST551-10mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×1ml |
| ST551-50mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×5ml |
| ST551-250mg | Puromycin Dihydrochloride (嘌呤霉素) | 250mg |
| ST1380-500mg | Polybrene (≥94%, Reagent grade) | 500mg |
| ST1380-2g | Polybrene (≥94%, Reagent grade) | 2g |
| ST1380-10g | Polybrene (≥94%, Reagent grade) | 10g |
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