产品编号 | 产品名称 | 产品包装 | 产品价格 |
L16500 | RPS6KA5基因敲除质粒 | 5µg | 999.00元 |
L16501 | RPS6KA5基因敲除慢病毒 | 10^8 TU | 6999.00元 |
L16502 | RPS6KA5基因敲除HEK293T细胞 | 1支/瓶 | 7999.00元 |
L16503 | RPS6KA5基因敲除HEK293T细胞RIPA裂解液 | 100µg | 1258.00元 |
L16504 | RPS6KA5基因敲除HEK293T细胞Trizol裂解液 | 500µl | 1258.00元 |
RPS6KA5 Knockout Lentivirus(RPS6KA5基因敲除慢病毒)是一种感染动物细胞后可以同时表达Cas9、目的基因sgRNA和puromycin抗性基因的慢病毒。本产品用于在动物细胞中基于CRISPR/Cas9技术敲除目的基因,并且本慢病毒中sgRNA的有效性已经通过T7EI法的验证。
本慢病毒基因序列的关键图谱信息请参考图1。本慢病毒可用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 可同时表达sgRNA、Cas9和puromycin抗性的本慢病毒其基因序列的关键图谱信息。
用于包装本慢病毒的质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得,sgRNA的有效性已经通过T7EI法验证。
本慢病毒用于实验时,建议同时选购无任何靶向的对照慢病毒Control Knockout Lentivirus (L00015)或靶向GFP的对照慢病毒GFP Knockout Lentivirus (L00017)。
碧云天同时提供基于CRISPR/Cas9技术的RPS6KA5基因敲除的质粒(L16500 pLenti-RPS6KA5-sgRNA)、慢病毒(L16501 RPS6KA5 Knockout Lentivirus)、HEK293T细胞(L16502 RPS6KA5 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L16503 RPS6KA5 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L16504 RPS6KA5 Knockout HEK293T Trizol Lysate)等产品,具体请在碧云天网站查询或在本产品网页点击相应产品。
RPS6KA5基因的基本信息如下:
Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
Human | RPS6KA5 | 9252 | BC017187 | NM_004755 |
About the gene | |
Official Symbol | RPS6KA5 |
Previous Symbol | - |
Official Full Name | ribosomal protein S6 kinase A5 |
Synonyms | MSK1; RLPK |
Location | 14q32.11 |
Gene Type | protein-coding gene |
Uniprot ID | O75582 |
Pathway/Library | Kinases Library |
Gene Summary | Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (PubMed:11909979, PubMed:12569367, PubMed:12763138, PubMed:9687510, PubMed:18511904, PubMed:9873047). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin (PubMed:11909979, PubMed:9873047). Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression (PubMed:12628924, PubMed:18511904). In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress (PubMed:12628924). In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential (PubMed:12763138). Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation (PubMed:12569367). Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A (PubMed:15010469). Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN (PubMed:12773393). May also phosphorylate 'Ser-28' of histone H3 (PubMed:12773393). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (PubMed:12773393). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (By similarity). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (By similarity). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity (PubMed:25851810). KS6A5_HUMAN,O75582 |
产品编号 | 产品名称 | 包装 |
L16501 | RPS6KA5 Knockout Lentivirus | 108 TU |
— | 说明书 | 1份 |
-80℃保存,至少一年有效。
注意事项:碧云天拥有sgRNA序列的知识产权,如果需要sgRNA序列,请在订购后发送邮件向info@beyotime.com索取。sgRNA序列信息与本慢病毒,未经碧云天书面许可不得用于任何商业用途,也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时,应注明来源。
对于非目录产品的CRISPR基因敲除用的慢病毒的定制,可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
产品编号 | 产品名称 | 包装 |
L00015 | Control Knockout Lentivirus | 108 TU |
L00017 | GFP Knockout Lentivirus | 108 TU |
C0222 | 青霉素-链霉素溶液(100X) | 100ml |
C0351-1ml | Polybrene (Hexadimethrine Bromide) | 1ml |
C0351-50mg | Polybrene (Hexadimethrine Bromide) | 50mg |
D0508S/M | 基因组编辑突变检测试剂盒 | 25/100次 |
D7080S/M/L | T7 Endonuclease I (CRISPR等基因突变鉴定用) | 250/1250/5000U |
ST551-10mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×1ml |
ST551-50mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×5ml |
ST551-250mg | Puromycin Dihydrochloride (嘌呤霉素) | 250mg |
ST1380-500mg | Polybrene (≥94%, Reagent grade) | 500mg |
ST1380-2g | Polybrene (≥94%, Reagent grade) | 2g |
ST1380-10g | Polybrene (≥94%, Reagent grade) | 10g |