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辣根过氧化物酶标记山羊抗兔IgG(H+L)

 产品编号A0208
 产品包装: 1ml
 产品价格: 193.00元
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产品简介

产品简介:

产品编号 产品名称 产品包装 产品价格
A0208 辣根过氧化物酶标记山羊抗兔IgG(H+L) 1ml 193.00元


来源

用途

WB

ELISA

IHC

Goat

WB, ELISA, IHC

1:1000

1:250

1:50

    WB, Western blot; ELISA, Enzyme-linked Immunosorbent Assay; IHC, Immunohistochemistry.
    本辣根过氧化物酶标记山羊抗兔IgG(H+L)(HRP-labeled Goat Anti-Rabbit IgG(H+L))为进口分装,用
于Western、ELISA和IHC等的二抗。HRP,即horseradish peroxidase,中文名为辣根过氧化物酶,可以在Western检测时催化ECL类试剂(如ECL、BeyoECL等)产生化学发光,可以在ELISA时催化TMB产生蓝色,也可以在IHC或Western blot检测时催化DAB产生棕色沉淀。
    本辣根过氧化物酶标记山羊抗兔IgG(H+L)用于WB、ELISA和IHC的推荐稀释比例参考上表,实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。对于WB,推荐的稀释范围为1:500-2000。
    本抗体为用纯化的兔IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化而获得的优质二抗。
    本抗体如果用于常规的Western检测,以每次检测需10毫升1:1000稀释的二抗计,至少可以检测100次。
包装清单:

产品编号

产品名称

包装

A0208

辣根过氧化物酶标记山羊抗兔IgG(H+L)

1ml

说明书

1份

保存条件:
    -20℃保存,一年有效。4℃保存,半年有效。
注意事项:
    叠氮钠会抑制辣根过氧化物酶的活性,在含有辣根过氧化物酶的二抗中不可加入叠氮钠。
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使用本产品的文献:
1. Wang YX, Xu XY, Su WL, Wang Q, Zhu WX, Chen F, Jin G, Liu YJ, Li YD, Sun YP, Gao WC,
   Ruan CP.
   Activation and Clinical Significance of p38 MAPK Signaling Pathway in Patients With
   Severe Trauma.

   J Surg Res. 2008 Dec 3.
2. Liu H, Li CQ, Ling BD, Zhou QX.
   Expression, purification and identification of LBD domain of human PPAR in E. coli.
   Journal of Medical Colleges of PLA 24 (2009) 76–83.
3. Yuan MJ, Huang CX, Tang YH, Wang X, Huang H, Chen YJ, Wang T.
   A novel peptide ghrelin inhibits neural remodeling after myocardial infarction in rats.
   Eur J Pharmacol. 2009 Sep 15;618(1-3):52-7.
4. Huang CX, Yuan MJ, Huang H, Wu G, Liu Y, Yu SB, Li HT, Wang T.
   Ghrelin inhibits post-infarct myocardial remodeling and improves cardiac function
   through anti-inflammation effect.

   Peptides. 2009 Dec;30(12):2286-91.
5. Chen J, Song M, Yu S, Gao P, Yu Y, Wang H, Huang L.
   Advanced glycation endproducts alter functions and promote apoptosis in endothelial
   progenitor cells through receptor for advanced glycation endproducts mediate
   overpression of cell oxidant stress.

   Mol Cell Biochem. 2010 Feb;335(1-2):137-46.
6. Gong F, Cheng X, Wang S, Zhao Y, Gao Y, Cai H.
   Heparin-immobilized polymers as non-inflammatory and non-thrombogenic coating materials
   for arsenic trioxide eluting stents.

   Acta Biomater. 2010 Feb;6(2):534-46. Epub 2009 Jul 14.
7. Sun J, Zhou W, Sha B, Yang Y.
   Ischemia induced neural stem cell proliferation and differentiation in neonatal rat
   involved vascular endothelial growth factor and transforming growth factor-beta    pathways.

   Brain Dev. 2010 Mar;32(3):191-200. Epub 2009 Feb 15.
8. Zeng J, Wang Q, Lin J, Deng K, Zhao X, Tang D, Liu X.
   Arabidopsis cryptochrome-1 restrains lateral roots growth by inhibiting auxin transport.
   J Plant Physiol. 2010;167(8):670-3. Epub 2010 Feb 4.
9. Wu J, Hu CP, Gu QH, Li YP, Song M.
   Trichostatin A sensitizes cisplatin-resistant A549 cells to apoptosis by up-regulating
   death-associated protein kinase.

   Acta Pharmacol Sin. 2010;31(1):93-101.
10.Liu HT, Li WM, Huang P, Chen WJ, Liu QS, Bai XF, Yu C, Du YG.
   Chitosan oligosaccharides inhibit TNF-a-induced VCAM-1 and ICAM-1 expression in human
   umbilical vein endothelial cells by blocking p38 and ERK1/2 signaling pathways.

   Carbohydrate Polymers.2010.
11.Wei CJ, Tian XF, Adam RD, Lu SQ.
   Giardia lamblia: intracellular localization of alpha8-giardin.
   Exp Parasitol. 2010;126(4):489-96. Epub 2010 May 31.
12.Wan YJ, Li YY, Xia W, Chen J, Lv ZQ, Zeng HC, Zhang L, Yang WJ, Chen T, Lin Y, Wei J, Xu
   SQ.
   Alterations in tumor biomarker GSTP gene methylation patterns induced by prenatal
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   Toxicology. 2010;274(1-3):57-64. Epub 2010 May 26.
13.Zhang P, Chao H, Sun X, Li L, Shi Q, Shen W.
  Murine folliculogenesis in vitro is stage-specifically regulated by insulin via the
   Akt signalingpathway.

   Histochem Cell Biol. 2010 Jul;134(1):75-82.
14.Zhao P, Tang SM, Liu T, Qin GX, Guo XJ.
   Expression and Activity Analysis of Non-Structural Protein 2 (NS2) of Bombyx mori
   Densovirus Zhenjiang Strain.

   Volume 9, Issue 12, December 2010, Pages 1821–1828.
15.Liu HT, Li WM, Li XY, Xu QS, Liu QS, Bai XF, Yu C, Du YG.
   Chitosan oligosaccharides inhibit the expression of interleukin-6 in
   lipopolysaccharide-inducedhuman umbilical vein endothelial cells through p38 and
   ERK1/2 protein kinases.

   Basic Clin Pharmacol Toxicol. 2010 May;106(5):362-71.
16.Li X, Xu Y, Ma L, Sun L, Fu G, et al.
   17β-Estradiol Enhances the Response of Plasmacytoid Dendritic Cell to CpG.
   PLoS One. 2009 Dec 23;4(12):e8412.
17.Zhang P, Chao HH, Sun XF, Li L, Shi QH, Shen W.
   Murine folliculogenesis in vitro is stage-specifically regulated by insulin via the
   Akt signaling pathway.

   Histochem Cell Biol. 2010 Jul;134(1):75-82.
18.Wang JF, Ruan KC.
   miR-335 is involved in the rat epididymal development by targeting the mRNA of
   RASA1.

   Biochem Biophys Res Commun. 2010 Nov 12;402(2):222-227.
19.Huang L, Qiu B, Yuan L, Zheng L, Li Q, Zhu S.
   Influence of fasting, insulin and glucose on ghrelin in the dorsal vagal complex in
   rats.

   J Endocrinol. 2011 Dec;211(3):257-62. Epub 2011 Sep 19.
20.Jing H, Qin J, Feng M, Wang T, Zhu J, Wang C, Wang F, Liu K, Li J, Liu C.
   Nitric oxide in enteric nervous system mediated the inhibitory effect of
   vasopressin on thecontraction of circular muscle strips from colon in male rats.

   Neurogastroenterol Motil. 2011 Mar;23(3):e125-35.
21.Huansheng D, Qingjie P, Hanqiong Z, Lianjun Z, Bo C, Wenbin Y.
   Estrogen inhibits the early development of mouse follicles through regulating the
   expression ofKit ligand.

   Biochem Biophys Res Commun. 2011 Jul 8;410(3):659-64.
22.Wang T, Zhang L, Shi C, Sun H, Wang J, Li R, Zou Z, Ran X, Su Y.
   TGF-β-induced miR-21 negatively regulates the antiproliferative activity but has
   no effect on EMTof TGF-β in HaCaT cells.

   Int J Biochem Cell Biol. 2012 Feb;44(2):366-76.Epub 2011 Nov 22.
23.Xiong Y, Wang J, Wu F, Li J, Zhou L, Kong L.
   Effects of (±)-praeruptorin A on airway inflammation, airway hyperresponsiveness
   and NF-κBsignaling pathway in a mouse model of allergic airway disease.

   Eur J Pharmacol. 2012 May 15;683(1-3):316-24. doi: 10.1016/j.ejphar.2012.03.004.
   Epub 2012 Mar 15.
24.Peng y, Li k, Pei RJ, Wu CC, Liang CY, Wang Y, Chen XW.
   The protamine-like DNA-binding protein P6.9 epigenetically up-regulatesAutographa
   californica multiple nucleopolyhedrovirus gene transcription in the late infection
   phase

   Virol Sin. 2012 Feb;27(1):57-68. doi: 10.1007/s12250-012-3229-x. Epub 2012 Jan 22.
25.Wu X, Zhao B, Li W, Chen Y, Liang R, Li L, Jin Y, Ruan K.
   MiR-200a is involved in rat epididymal development by targeting β-catenin mRNA.
   Acta Biochim Biophys Sin (Shanghai).2012 Mar;44(3):233-40.doi:10.1093/abbs/gmr122.
   Epub 2012 Jan 11.
26.Yu L, Lu M, Wang P, Chen X.
   Trichostatin A Ameliorates Myocardial Ischemia/Reperfusion Injury Through
   Inhibition ofEndoplasmic Reticulum Stress-induced Apoptosis.

   Arch Med Res. 2012 May 4, S0188-4409(12)00123-3.
27.Feng YM, Zou ZM, Guo L, Zhang X, Wang T, Sun HP, Liu Y, chen XH.
   Umbilical Cord Blood-Derived Stromal Cells Regulate Megakaryocytic Proliferation
   and Migration Through SDF-1/PECAM-1 Pathway.

   Cell Biochemistry and Biophysics 2012, DOI: 10.1007/s12013-012-9362-1.
28.Zhang C, Lin G, Wan W, Li X, Zeng B, Yang B, Huang C.
   Resveratrol, a polyphenol phytoalexin, protects cardiomyocytes against
   anoxia/reoxygenation injury via theTLR4/NF-κB signaling pathway.

   Int J Mol Med. 2012 Apr;29(4):557-63. doi: 10.3892/ijmm.2012.885. Epub 2012 Jan 11.
29.Li Y, Xiang YY, Lu WY, Liu C, Li J.
   A novel role of intestine epithelial GABAergic signaling in regulating intestinal
   fluid secretion.

   Am J Physiol Gastrointest Liver Physiol. 2012 Aug 15;303(4):G453-60.
   doi: 10.1152/ajpgi.00497.2011. Epub 2012 Jun 14.
30.Feng X, Wang H, Ye S, Guan J, Tan W, Cheng S, Wei G, Wu W, Wu F, Zhou Y.
   Up-regulation of microRNA-126 may contribute to pathogenesis of ulcerative colitis
   via regulating NF-kappaBinhibitor IκBα.

   PLoS One. 2012;7(12):e52782. doi: 10.1371/journal.pone.0052782. Epub 2012 Dec 28.
31.Zhang LJ, Pan B, Chen B, Zhang XF, Liang GJ, Feng YN, Wang LQ, Ma JM, Li L, Shen W.
   Expression and epigenetic dynamics of transcription regulator Lhx8 during mouse
   oogenesis.

   Gene. 2012 Sep 10;506(1):1-9. doi: 10.1016/j.gene.2012.06.093. Epub 2012 Jul 10.
32.Zhang ZP, Liang GJ, Zhang XF, Zhang GL, Chao HH, Li L, Sun XF, Min LJ, Pan QJ, Shi QH,
   Sun QY, De Felici M, Shen W.
   Growth of mouse oocytes to maturity from premeiotic germ cells in vitro.
   PLoS One. 2012;7(7):e41771. doi: 10.1371/journal.pone.0041771. Epub 2012 Jul 24.
33.Yang Y, Huang Q, Lu Y, Li X, Huang S.
   Reactivating PP2A by FTY720 as a novel therapy for AML with C-KIT tyrosine kinase
   domain mutation.

   J Cell Biochem. 2012 Apr;113(4):1314-22. doi: 10.1002/jcb.24003.
34.Ma Z, Zhang H, Zheng J, Li Y, Yi L, Fan H, Lu C.
   Interaction between M-like protein and macrophage thioredoxin facilitates
   antiphagocytosis for Streptococcusequi ssp. zooepidemicus.

   PLoS One. 2012;7(2):e32099. doi: 10.1371/journal.pone.0032099. Epub 2012 Feb 27.
35.Zhang HQ, Zhang XF, Zhang LJ, Chao HH, Pan B, Feng YM, Li L, Sun XF, Shen W.
   Fetal exposure to bisphenol A affects the primordial follicle formation by inhibiting
   the meiotic progression ofoocytes.

   Mol Biol Rep. 2012 May;39(5):5651-7. doi: 10.1007/s11033-011-1372-3. Epub 2011 Dec 21.
36.Lin S, Zhang J, Chen H, Chen K, Lai F, Luo J, Wang Z, Bu H, Zhang R, Li H, Tong H.
   Involvement of endoplasmic reticulum stress in capsaicin-induced apoptosis of human
   pancreatic cancer cells.

   Evid Based Complement Alternat Med. 2013;2013:629750. doi: 10.1155/2013/629750.
   Epub 2013 May 28.
37.Zhu YM, Wang CC, Chen L, Qian LB, Ma LL, Yu J, Zhu MH, Wen CY, Yu LN, Yan M.
   Both PI3K/Akt and ERK1/2 pathways participate in the protection by dexmedetomidine
   against transient focalcerebral ischemia/reperfusion injury in rats.

   Brain Res. 2013 Feb 4;1494:1-8. doi: 10.1016/j.brainres.2012.11.047. Epub 2012 Dec 5.
38.Zhou TF, Yu JG.
   Recombinant human erythropoietin attenuates neuronal apoptosis and cognitive defects
   via JAK2/STAT3signaling in experimental endotoxemia.

   J Surg Res. 2013 Jul;183(1):304-12. doi: 10.1016/j.jss.2012.11.035. Epub 2012 Dec 6.
39.Xie J, Wang C, Yang A, Zhang B, Yin Q, Huang H, Chen M.
   Cucurmosin Kills Human Pancreatic Cancer SW-1990 Cells in vitro and in vivo.
   Anticancer Agents Med Chem. 2013 Jul 1;13(6):952-6.
40.Zhou B.
   Heat stress-induced PPAR-? enhances HUVEC resistance to oxidant injury.
   Asian Biomedicine. Vol. 7 No. 2 April 2013.
41.Liu F, Xie M, Chen D, Li J, Ding W.
   Effect of V(IV)O(dipic-Cl)(H2O)2 on Lipid Metabolism Disorders in the Liver of
   STZ-Induced Diabetic Rats.

   J Diabetes Res. 2013;2013:956737. doi: 10.1155/2013/956737. Epub 2013 Mar 20.
42.Wang P, Wang X, Yang X, Liu Z, Wu M, Li G.
   Budesonide suppresses pulmonary antibacterial host defense by down-regulating
   cathelicidin-related antimicrobial peptide in allergic inflammation mice and in lung
   epithelial cells.

   BMC Immunol. 2013 Feb 6;14:7. doi: 10.1186/1471-2172-14-7.
43.Li WL, Chen ML, Liu SS, Li GL, Gu TY, Liang P, Qin YM, Zhan YH, Quan Y, Zhang GH.
   Sweet Preference Modified by Early Experience in Mice and the Related Molecular
   Modulations on the PeripheralPathway.

   J Mol Neurosci. 2013 Apr 19.
44.Hu Z, Xing Y, Qian Y, Chen X, Tu J, Ren L, Wang K, Chen Z.
   Anti-radiation damage effect of polyethylenimine as a toll-like receptor 5 targeted
   agonist.

   J Radiat Res. 2013 Mar 1;54(2):243-50. doi: 10.1093/jrr/rrs098. Epub 2012 Oct 26.
45.Zhang F, Li Y, Zhang L, Mu G.
   Synergistic protective effects of escin and low-dose glucocorticoids on blood-retinal
   barrier breakdown in a rat model of retinal ischemia.

   Mol Med Rep. 2013 May;7(5):1511-5. doi: 10.3892/mmr.2013.1374. Epub 2013 Mar 15.
46.Lin Y, Meng Y, Wang YX, Luo J, Katsuma S, Yang CW, Banno Y, Kusakabe T, Shimada T,
   Xia QY.
   Vitellogenin receptor mutation leads to the oogenesis mutant phenotype "scanty
   vitellin" of the silkworm,Bombyx mori.

   J Biol Chem. 2013 May 10;288(19):13345-55. doi: 10.1074/jbc.M113.462556. Epub 2013
   Mar 20.
47.Gu Y,Zhao Y, Gong L, Wang Y, Zhang Y.
   Potassium aspartate attenuates apoptotic cell death after focal cerebral ischemia in
   rats
.
   Biomedicine & Aging Pathology.Volume 3, Issue 1, January–March 2013,
   Pages 14–19.
48.Jin XL, Sun QS, Liu F, Yang HW, Liu M, Liu HX, Xu W, Jiang YY.
   microRNA 21-mediated suppression of Sprouty1 by Pokemon affects liver cancer cell
   growth and proliferation.

   J Cell Biochem. 2013 Jul;114(7):1625-33. doi: 10.1002/jcb.24504.
49.Pan Y, Shu JL, Gu HF, Zhou DC, Liu XL, Qiao QY, Fu SK, Gao FH, Jin HM.
   Erythropoietin improves insulin resistance via the regulation of its
   receptor-mediated signaling pathways in 3T3L1adipocytes.

   Mol Cell Endocrinol. 2013 Mar 10;367(1-2):116-23. doi: 10.1016/j.mce.2012.12.027.
   Epub 2013 Jan 11.
50.Zhan C, Shi Y, Lu C, Wang Q.
   Pyruvate kinase M2 is highly correlated with the differentiation and the prognosis
   of esophageal squamous cellcancer.

   Dis Esophagus. 2013 Jan 14. doi: 10.1111/dote.12023.
51.Cai C, Wang C, Ji W, Liu B, Kang Y, Hu Z, Jiang Z.
   Knockdown of hepatic aquaglyceroporin-9 alleviates high fat diet-induced non-alcoholic
   fatty liver disease in rats.

   Int Immunopharmacol. 2013 Mar;15(3):550-6. doi: 10.1016/j.intimp.2013.01.020.
   Epub 2013 Feb 14.
52.Luo W, Liu Z, Tan D, Zhang Q, Peng H, Wang Y, Tan Y.
   Gamma-amino butyric acid and the A-type receptor suppress decidualization of mouse
   uterine stromal cells bydown-regulating cyclin D3.

   Mol Reprod Dev. 2013 Jan;80(1):59-69. doi: 10.1002/mrd.22137. Epub 2012 Dec 27.
53.Tang C, Yang L, Jiang X, Xu C, Wang M, Wang Q, Zhou Z, Xiang Z, Cui H.
   Antibiotic drug tigecycline inhibited cell proliferation and induced autophagy in
   gastric cancer cells.

   Biochem Biophys Res Commun. 2014 Mar 28;446(1):105-12. doi: 10.1016/j.bbrc.
   2014.02.043. Epub 2014 Feb 28.
54.Zhu W, Chen S, Li Z, Zhao X, Li W, Sun Y, Zhang Z, Ling W, Feng X.
   Effects and mechanisms of resveratrol on the amelioration of oxidative stress and
   hepatic steatosis in KKAy mice.

   Nutr Metab (Lond). 2014 Aug 12;11:35. doi: 10.1186/1743-7075-11-35. eCollection 2014.
55.Ren T, Qiu Y, Wu W, Feng X, Ye S, Wang Z, Tian T, He Y, Yu C, Zhou Y.
   Activation of adenosine A3 receptor alleviates TNF-α-induced inflammation through
   inhibition of the NF-κB signaling pathway in human colonic epithelial cells.

   Mediators Inflamm. 2014;2014:818251. doi: 10.1155/2014/818251. Epub 2014 Apr 22.
56.Khan MK, He L, Zhang W, Wang Y, Tao Q, Song Q, Sajid MS, Yu Q, Hu J, Fang R, Hu M, Zhou
   Y, Zhao J.
   Identification of two novel HSP90 proteins in Babesia orientalis: molecular
   characterization, and computational analyses of their structure, function, antigenicity
   and inhibitor interaction.

   Parasit Vectors. 2014 Jun 26;7:293. doi: 10.1186/1756-3305-7-293.
57.Yu Y, Tong Q, Li Z, Tian J, Wang Y, Su F, Wang Y, Liu J, Zhang Y.
   Improved site-specific recombinase-based method to produce selectable marker- and
   vector-backbone-free transgenic cells.

   Sci Rep. 2014 Feb 28;4:4240. doi: 10.1038/srep04240.
58.Deng C, Qin A, Zhao W, Feng T, Shi C, Liu T.
   Up-regulation of CXCR4 in rat umbilical mesenchymal stem cells induced by serum from
   rat with acute liver failure promotes stem cells migration to injured liver tissue.

   Mol Cell Biochem. 2014 Aug 7.



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