BeyoECL Plus(超敏ECL化学发光试剂盒)

 产品编号: P0018
 产品包装: 共100ml
 产品价格: 278.00元
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产品简介

产品简介:

产品编号 产品名称 产品包装 产品价格
P0018 BeyoECL Plus(超敏ECL化学发光试剂盒) 共100ml 278.00元

    碧云天生产的Western萤光检测试剂BeyoECL Plus是一种超敏ECL化学发光试剂盒,与高灵敏度的ECL Plus非常相似的萤光检测试剂,可与二抗上耦连的辣根过氧化物酶发生化学反应,发出萤光,从而可以通过用X光片压片或其它适当萤光成像设备检测样品。
      BeyoECL Plus灵敏度极高,比DAB显色的灵敏度至少高200倍,比Amersham公司的ECL或碧云天以前生产的BeyoECL的灵敏度高20-50倍左右,比Pierce公司的SuperSignal West Pico Substrate的灵敏度高3-100倍。
    BeyoECL Plus的发光时间更长。在100微升1微克/毫升辣根过氧化物酶标记IgG中,加入100微升碧云天的BeyoECL Plus工作液或Pierce公司的SuperSignal West Pico Substrate工作液进行检测。在暗房中,两者均可发出肉眼可见的蓝色萤光,但加入BeyoECL工作液的试管内的亮度明显高很多。最初数分钟内加入BeyoECL Plus工作液的试管发光强度是加入SuperSignal West Pico Substrate工作液的试管的3倍。约30分钟后,加入BeyoECL Plus工作液的试管仍然可以发出肉眼可见的较强的蓝色萤光,而加入Pierce公司的SuperSignal West Pico Substrate工作液的试管发出的萤光已经很难用肉眼进行分辨,定量结果显示此时加入BeyoECL Plus工作液的试管发光强度是加入SuperSignal West Pico Substrate工作液的试管的100倍以上(参考图1)。


图1. BeyoECL Plus和Pierce公司SuperSignal West Pico效果比较图。详细的比较方法如上所述。

    BeyoECL Plus的高灵敏度可以大大节省宝贵的样品以及非常昂贵的一抗和二抗的用量。同时必须注意适当减少一抗和二抗或样品的用量,以免获得过深的难以比较灰度的条带。
    BeyoECL Plus采用了全新配方,更加稳定。BeyoECL Plus的两种试剂37℃水浴孵育30天,和4℃保存的试剂相比,对相同样品的检测效果无任何明显差异。
    BeyoECL Plus的背景很低,对PVDF膜和硝酸纤维素膜均适用。PVDF膜(FFP36/FFP39)硝酸纤维素膜(FFN06)可以向碧云天订购。
包装清单:

产品编号 产品名称 包装
P0018-1 BeyoECL Plus A液 50ml
P0018-2 BeyoECL Plus B液 50ml
说明书 1份

保存条件:
    4℃避光保存,一年有效。如果长期不用,可以-20℃保存。-20℃可以保存更长时间。
注意事项:
    BeyoECL Plus A液和B液在吸取过程中必须要更换枪头,A液和B液相互污染后会导致A液或B液逐渐失效,影响后续的使用效果。
    各溶液使用后,请盖紧瓶盖,以防失效。特别是B液,含有氧化剂,比较容易被还原而失效。
    BeyoECL Plus的萤光持续时间很长,但开始反应后的30分钟内萤光更强一些,随后萤光会逐渐减弱,因此请注意充分利用这萤光较强的30分钟进行压片。
    在BeyoECL Plus的检测灵敏度仍不足以满足实验需要时,请选择检测灵敏度更高的BeyoECL Star (P0018A)。
    BeyoECL Plus A液和B液均对人体有害,操作时请注意适当防护。
    本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
    为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明

使用说明:
1. Western实验步骤可以参考碧云天的相关网页:http://www.beyotime.com/western.htm
2. BeyoECL Plus工作液的配制等体积混合适量BeyoECL Plus A液和B液,室温放置备用。工作液宜在
   临检测前配制。
3. Western二抗孵育后,并进行数次洗涤后,用平头镊将膜取出,用吸水纸略吸去过多的液体(切勿接触膜
   的蛋白面),然后置于一洁净保鲜膜上。
4. 根据膜的大小,按每10平方厘米膜加1ml BeyoECL Plus工作液的比例,滴加BeyoECL Plus工作液到膜
   上,确保使工作液均匀覆盖在膜上,放置1-2分钟。
5. 取膜,弃BeyoECL Plus工作液,用吸水纸略吸去过多的液体。将膜放在两片保鲜膜中间,随后进行压片
   检测或萤光成像仪检测。
6. 压片检测:将膜固定于片夹内。暗室内压片1分钟,立即显影定影,根据结果再调整压片时间。或直接
   分别压片30秒,1、3、5分钟,然后一起显影定影观察结果。
7. 萤光成像仪检测:将膜放置到萤光成像仪内,参考仪器说明书进行检测。

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   Protection of mice against enterotoxigenic E. coli by immunization with a polyvalent
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   Appl Microbiol Biotechnol. 2011 Mar;89(6):1885-93.
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77.Wang Z, Yan J, Zheng Q, Wang Z, Li J, Zhang X, Zhang S.
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78.Ling Y, Chunli L, Xiaohou W, Qiaoling Z.
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   The failure to express a protein disulphide isomerase-like protein results in a floury
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83.Su F, Yuan L, Zhang L, Hu S
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84.Zhou W, Xiong Z, Liu Y, Yao C, Li C.
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86.Xu JY, Zhu WJ, Jiang H.
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   The anti-fibrotic effect of betulinic acid is mediated through the inhibition of NF-κB
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90.Miao M, Niu Y, Xie T, Yuan B, Qing C, Lu S.
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   Anticancer potential of aqueous extract of alocasia macrorrhiza against hepatic cancer
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92.Dong H, Mao S, Wei J, Liu B, Zhang Z, Zhang Q, Yan M.
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   Cloning and characterization of prophenoloxidase A3 (proPOA3) from Culex pipiens
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   Cell Signal. 2012 Jun;24(6):1134-40.
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   Characteristic comparison of three rat models induced by cigarette smoke or combined
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   HBx activates FasL and mediates HepG2 cell apoptosis through MLK3-MKK7-JNKs signal
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   World J Gastroenterol. 2012 Apr 7;18(13):1485-95.
101.Zhao D, Lin F, Wu X, Zhao Q, Zhao B, Lin P, Zhang Y, Yu X.
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   Toxicol In Vitro. 2012 Jun;26(4):595-602.
102.Du Z, Yang Y, Hu Y, Sun Y, Zhang S, Peng W, Zhong Y, Huang X, Kong W.
   A long-term high-fat diet increases oxidative stress, mitochondrial damage and
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   Hear Res. 2012 May;287(1-2):15-24.
103.Wu J, Zhu W, Fu H, Zhang Y, Sun J, Yang W, Li Y.
   DNA-PKcs interacts with Aire and regulates the expression of toll-like receptors in
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   Scand J Immunol. 2012 May;75(5):479-88.
104.Shi ZJ, Ouyang DY, Zhu JS, Xu LH, He XH.
   Histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits
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   Int Immunopharmacol. 2012 Apr;12(4):580-7.
105.Chen C, Chen C, Wang Z, Wang L, Yang L, Ding M, Ding C, Sun Y, Lin Q, Huang X, Du X,
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106.Tao SF, Zhang CS, Guo XL, Xu Y, Zhang SS, Song JR, Li R, Wu MC, Wei LX.
   Anti-tumor effect of 5-aza-2'-deoxycytidine by inhibiting telomerase activity in
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   World J Gastroenterol. 2012 May 21;18(19):2334-43.
107.Zhang Y, Xu Y, Zhu Q, Zhao F, Luo J, Zhang X, Wang X.
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   Synapse. 2012 Jul;66(7):622-9. doi: 10.1002/syn.21548.
108.Zhang LJ, Pan B, Chen B, Zhang XF, Liang GJ, Feng YN, Wang LQ, Ma JM, Li L, Shen W.
   Expression and epigenetic dynamics of transcription regulator Lhx8 during mouse
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   Gene. 2012 Sep 10;506(1):1-9.
109.Lian LH, Jin Q, Song SZ, Wu YL, Bai T, Jiang S, Li Q, Yang N, Nan JX.
   Ginsenoside Rh2 Downregulates LPS-Induced NF-κB Activation through Inhibition of TAK1
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   Evid Based Complement Alternat Med. 2013;2013:646728. doi: 10.1155/2013/646728.
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110.Yasen M, Fei Q, Hutton WC, Zhang J, Dong J, Jiang X, Zhang F.
   Changes of number of cells expressing proliferation and progenitor cell markers with
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   Acta Biochim Biophys Sin (Shanghai). 2013 May;45(5):368-76.
111.Liu XL, Li G, Wang ZH, Zhao WJ, Wang LP.
   Increased expression of Dock180 protein in the noninfarcted myocardium in rats.
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112.Zhang X, Li G, Zhang L, Yang D, Zhang Z, Yan A, Linghu H.
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   Cell Biol Int. 2013 May 17. doi: 10.1002/cbin.10136.
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   Thymoquinone attenuates liver fibrosis via PI3K and TLR4 signaling pathways in
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   Int Immunopharmacol. 2013 Feb;15(2):275-81.
114.Yin H, Guo C, Wang Y, Liu D, Lv Y, Lv F, Lu Z.
   Fengycin inhibits the growth of the human lung cancer cell line 95D through reactive
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   Anticancer Drugs. 2013 Jul;24(6):587-98. doi: 10.1097/CAD.0b013e3283611395.
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   Positive feedback regulation of stgR expression for secondary metabolism in Streptomyces
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   Int J Mol Med. 2013 Jul;32(1):130-6. doi: 10.3892/ijmm.2013.1370. Epub 2013 May 8.
117.Wang XQ, Hu GH, Kang HY, Shen Y, Hong SL.
   Significance of the IL-6 pathway in nasal polyposis in Chinese patients.
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118.Luo B, Jiang M, Yang X, Zhang Z, Xiong J, Schluesener HJ, Zhang Z, Wu Y.
   Erythropoietin is a hypoxia inducible factor-induced protective molecule in experimental
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119.Li BL, Lu C, Lu W, Yang TT, Qu J, Hong X, Wan XP.
   miR-130b is an EMT-related microRNA that targets DICER1 for aggression in endometrial
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   CD14 overexpression upregulates TNF-α-mediated inflammatory responses and suppresses
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121.Zhang YT, Ouyang DY, Xu LH, Zha QB, He XH.
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122.Zhang S, Qi Y, Zhang H, He W, Zhou Q, Gui S, Wang Y.
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   J Asthma. 2013 Jun;50(5):439-48. doi: 10.3109/02770903.2013.778275. Epub 2013 May 7.
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   A gain-of-function mutation in IAA8 alters Arabidopsis floral organ development by
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128.Wang C, Ma W, Su Y.
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129.Chu X, You H, Yuan X, Zhao W, Li W, Guo X.
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130.Pan Y, Shu JL, Gu HF, Zhou DC, Liu XL, Qiao QY, Fu SK, Gao FH, Jin HM.
   Erythropoietin improves insulin resistance via the regulation of its receptor-mediated
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   Mol Cell Endocrinol. 2013 Mar 10;367(1-2):116-23. doi: 10.1016/j.mce.2012.12.027.
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131.Zhang C, Zhao J, Deng H.
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   Mol Cell Biochem. 2013 Jul;379(1-2):201-11. doi: 10.1007/s11010-013-1642-6.
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133.Zhang Z, Wang H, Sun Y, Li H, Wang N.
   Klf7 modulates the differentiation and proliferation of chicken preadipocyte.
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134.Jia Q, Zhang X, Deng T, Gao J.
   Positive correlation of Oct4 and ABCG2 to chemotherapeutic resistance in CD90(+)CD133(+)
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   Cell Reprogram. 2013 Apr;15(2):143-50. doi: 10.1089/cell.2012.0048. Epub 2013 Feb 25.
135.Ku Z, Ye X, Huang X, Cai Y, Liu Q, Li Y, Su Z, Huang Z.
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136.Cai Y, Liu Q, Huang X, Li D, Ku Z, Zhang Y, Huang Z.
   Active immunization with a Coxsackievirus A16 experimental inactivated vaccine induces
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   Vaccine. 2013 Apr 26;31(18):2215-21. doi: 10.1016/j.vaccine.2013.03.007.
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137.Gao L, Song JR, Zhang JW, Zhao X, Zhao QD, Sun K, Deng WJ, Li R, Lv G, Cheng HY,
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   Chloroquine promotes the anticancer effect of TACE in a rabbit VX2 liver tumor model.
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138.He J, Wang Y, Xu LH, Qiao J, Ouyang DY, He XH.
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   Int Immunopharmacol. 2013 May;16(1):27-34. doi: 10.1016/j.intimp.2013.03.013.
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139.Lin C, Wang L, Wang H, Yang L, Guo H, Wang X.
   Tanshinone II A inhibits breast cancer stem cells growth in vitro and in vivo through
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140.Yu Y, Schachner M.
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141.Liu J, Wang Y, Du W, Liu W, Liu F, Zhang L, Zhang M, Hou M, Liu K, Zhang S, Yu B.
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142.Wang CM, Liu MY, Wang F, Wei MJ, Wang S, Wu CF, Yang JY.
   Anti-amnesic effect of pseudoginsenoside-F11 in two mouse models of Alzheimer's disease.
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143.Liu Y, Wang DK, Jiang DZ, Qin X, Xie ZD, Wang QK, Liu M, Chen LM.
   Cloning and functional characterization of novel variants and tissue-specific expression
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144.Luo W, Liu Z, Tan D, Zhang Q, Peng H, Wang Y, Tan Y.
   Gamma-amino butyric acid and the A-type receptor suppress decidualization of mouse
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   Mol Reprod Dev. 2013 Jan;80(1):59-69. doi: 10.1002/mrd.22137. Epub 2012 Dec 27.
145.Li Z, Sun C, Zhang T, Mo J, Shi Q, Zhang X, Yuan M, Chen L, Mao X, Yu R, Zhou X.
   Geranylgeranyltransferase I mediates BDNF-induced synaptogenesis.
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146.Wang L, Guo H, Yang L, Dong L, Lin C, Zhang J, Lin P, Wang X.
   Morusin inhibits human cervical cancer stem cell growth and migration through
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   Mol Cell Biochem. 2013 Jul;379(1-2):7-18. doi: 10.1007/s11010-013-1621-y.
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147.Ouyang DY, Xu LH, He XH, Zhang YT, Zeng LH, Cai JY, Ren S.
   Autophagy is differentially induced in prostate cancer LNCaP, DU145 and PC-3 cells via
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   AtMYB44 regulates resistance to the green peach aphid and diamondback moth by activating
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   Plant Biol (Stuttg). 2013 May 8. doi: 10.1111/j.1438-8677.2012.00675.x.
149.Liu B, Chen Q, Tian D, Wu L, Dong H, Wang J, Ji B, Zhu X, Cai Q, Wang L, Zhang S.
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150.Chen L, Han F, Qu H, Yan H, Ren L, Yang S.
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   Pharmazie. 2013 Feb;68(2):117-23.
151.Zhu S, Liu W, Ke X, Li J, Hu R, Cui H, Song G.
   Artemisinin reduces cell proliferation and induces apoptosis in neuroblastoma.
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152.Cao FF, Xu LM, Zhang X, Wang Y, Li MQ, Uzan G, Peng B, Zhang DH.
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153.Du Z, Yang Q, Zhou T, Liu L, Li S, Chen S, Gao C.
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154.Hu LT, Du ZD, Zhao GQ, Jiang N, Lin J, Wang Q, Xu Q, Cong L, Qiu S.
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155.Lu Y, Long M, Zhou S, Xu Z, Hu F, Li M.
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156.Lu B, Shao L, Feng S, Wang T, Zhong Y.
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157.Lu X, Chen Y, Zeng T, Chen L, Shao Q, Qin W.
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158.Wang Y, Liu D, Zhao H, Jiang H, Luo C, Wang M, Yin H.
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159.Wang ZC, Sun HJ, Li KH, Fu C, Liu MZ.
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160.Gao L, Qi HB, Kc K, Zhang XM, Zhang H, Baker PN.
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   The role of PinX1 in growth control of breast cancer cells and its potential molecular
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162.Zhang Y, Zhu S, Yi L, Liu Y, Cui H.
   Neurotensin receptor1 antagonist SR48692 reduces proliferation by inducing apoptosis
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163.Linghu L, Fan H, Hu Y, Zou Y, Yang P, Lan X, Liao Z, Chen M.
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