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Bradford蛋白浓度测定试剂盒

 产品编号: P0006
 产品包装: 1000次
 产品价格: 106.00元
说明书下载
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产品简介

产品简介:

产品编号 产品名称 产品包装 产品价格
P0006

Bradford蛋白浓度测定试剂盒

1000次 106.00元

    Bradford蛋白浓度测定试剂盒(Bradford Protein Assay Kit)是根据最常用的两种蛋白浓度检测方法之一Bradford法研制而成,实现了蛋白浓度测定的快速,稳定和高灵敏度。
    检测速度极快,10-20个样品只需不足10分钟即可完成。
    灵敏度高,检测浓度下限达到25μg/ml,最小检测蛋白量达到0.5μg,待测样品体积为1-20μl
    在50-1000μg/ml浓度范围内有较好的线性关系。
    Bradford法测定蛋白浓度不受绝大部分样品中的化学物质的影响。样品中β-巯基乙醇的浓度可高达
1M,二硫苏糖醇的浓度可高达5mM。但受略高浓度的去垢剂影响。需确保SDS低于0.01%,Triton X-100低于0.05%,Tween 20,60,80低于0.015%。含去垢剂的样品推荐使用碧云天生产的BCA蛋白浓度测定试剂盒
(P0012)

    Bradford蛋白浓度测定试剂盒对样品中各种物质的详细的兼容性,请参见碧云天如下网页:
    http://www.beyotime.com/Compatibility Chart For Bradford Kit.pdf
    每个试剂盒可以检测1000个样品。
包装清单:

产品编号

产品名称

包装

P0006-1

G250染色液

100ml×2

P0006-2

蛋白标准(5mg/ml BSA)

1ml

说明书

1份

保存条件:
    G250染色液4℃保存,蛋白标准-20℃保存,本试剂盒自订购之日起九个月内有效。
注意事项:
    G250染色液使用前请颠倒3-5次,混匀
    蛋白标准请在全部溶解后先混匀,再稀释成一系列不同浓度的蛋白标准。
    将G250染色液回复到室温再使用,有利于提高检测的灵敏度。
    需可检测560-610nm之间波长的酶标仪一台,最佳检测波长为595nm。并需96孔板。
    为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明

使用说明:
1. 完全溶解蛋白标准品,取10μl稀释至100μl,使终浓度为0.5mg/ml。蛋白样品在什么溶液中,标准品也
   宜用什么溶液稀释。但是为了简便起见,也可以用0.9%NaCl或PBS稀释标准品。
2. 将标准品按0, 1, 2, 4, 8, 12, 16, 20μl加到96孔板的标准品孔中,加标准品稀释液足到20μl
3. 加适当体积样品到96孔板的样品孔中,加标准品稀释液到20μl
4. 各孔加入200μl G250染色液室温放置3-5分钟
5. 用酶标仪测定A595,或560-610nm之间的其它波长的吸光度。
6. 根据标准曲线计算出样品中的蛋白浓度。

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79.Wang JJ, Li YF, Jin YY, Wang X, Chen TX.
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82.Zhu S, Wang Y, Chen M, Jin J, Qiu Y, Huang M, Huang Z.
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83.Tan Y, Xing J, Zhan W.
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85.Wu S, Ju GQ, Du T, Zhu YJ, Liu GH.
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87.Ma J, Zhang B, Liu S, Xie S, Yang Y, Ma J, Deng Y, Wang W, Xu L, Li R, Zhang L, Yu C,
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88.Li K, Zhang L, Xiang X, Gong S, Ma L, Xu L, Wang G, Liu Y, Ji X, Liu S, Chen P, Zeng H,
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89.Wang J, Cui J, Zhu H.
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90.Ma W, Kang F, Wang Z, Yang W, Li G, Ma X, Li G, Chen K, Zhang Y, Wang J.
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   characterization.

   Appl Biochem Biotechnol.2013 Feb;169(3):870-84.doi: 10.1007/s12010-012-9936-9.
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   Basal caspase-3 activity promotes migration, invasion, and vasculogenic mimicry
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   Melanoma Res.2013 Aug;23(4):243-53.doi: 10.1097/CMR.0b013e3283625498.
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97.Miao Y, Zhou J, Zhao M, Liu J, Sun L, Yu X, He X, Pan X, Zang W.
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   Cell Physiol Biochem.2013;31(2-3):189-98.doi: 10.1159/000343360.Epub 2013 Feb 8.
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   Mol Med Rep.2013 Aug;8(2):505-10.doi: 10.3892/mmr.2013.1506.Epub 2013 May 31.
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   World Congress on Medical Physics and Biomedical Engineering May 26-31, 2012, Beijing,
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103.Hao Y, Ren J, Liu C, Li H, Liu J, Yang Z, Li R, Su Y.
   Zinc Protects Human Kidney Cells from Depleted Uranium-induced Apoptosis.
   Basic Clin Pharmacol Toxicol. 2013 Oct 29. doi: 10.1111/bcpt.12167.
104.Jiang ZC, Tang XM, Zhao YR, Zheng L.
   A functional variant at miR-34a binding site in toll-like receptor 4 gene alters
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   Tumour Biol. 2014 Sep 2.
105.Li L, Zhao F, Lu J, Li T, Yang H, Wu C, Liu Y.
   Notch-1 signaling promotes the malignant features of human breast cancer through NF-κB
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   PLoS One. 2014 Apr 23;9(4):e95912. doi: 10.1371/journal.pone.0095912. eCollection
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106.Pan S, Hu J, Zheng T, Liu X, Ju Y, Xu C.
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   Cytotechnology. 2014 Apr 12.
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108.Han RF, Li K, Yang ZS, Chen ZG, Yang WC.
   Trichostatin A induces mesenchymal-like morphological change and gene expression but
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   Mol Med Rep. 2014 Dec;10(6):3211-6. doi: 10.3892/mmr.2014.2594. Epub 2014 Sep 23.
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   The effect of gold nanoparticles on the proliferation and differentiation of murine
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   J Nanosci Nanotechnol. 2014 Jul;14(7):4851-7.
110.Li M, Sun L, Li Y, Xie C, Wan D, Luo Y.
   Oxygen glucose deprivation/reperfusion astrocytes promotes primary neural stem/
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   Neurochem Res. 2014 Aug;39(8):1440-50. doi: 10.1007/s11064-014-1333-z. Epub 2014 May
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   Microvesicles mediate transfer of P-glycoprotein to paclitaxel-sensitive A2780 human
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   Eur J Pharmacol. 2014 Sep 5;738:83-90. doi: 10.1016/j.ejphar.2014.05.026. Epub 2014
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112.Yang Y, Qiu Y, Wang W, Xiao W, Liang H, Zhang C, Yang H, Teitelbaum DH, Sun LH, Yang H.
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   Int J Clin Exp Pathol. 2014 Apr 15;7(5):2006-18. eCollection 2014.
113.Li M, Sun L, Luo Y, Xie C, Pang Y, Li Y.
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   Int J Mol Med. 2014 Sep;34(3):705-14. doi: 10.3892/ijmm.2014.1820. Epub 2014 Jun 24.
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   Bufalin induces cell cycle arrest and apoptosis in gallbladder carcinoma cells.
   Tumour Biol. 2014 Aug 2.



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