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产品简介
产品简介：
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| C1038 | DiO(细胞膜绿色荧光探针) | 10mg | 441.00元 |
DiO即DiOC18(3)，全称为3,3′-dioctadecyloxacarbocyanine perchlorate，是最常用的细胞膜荧
光探针之一，呈现绿色荧光。DiO是一种亲脂性膜染料，进入细胞膜后可以侧向扩散逐渐使整个细胞的细胞膜被染色。
DiO在进入细胞膜之前荧光非常弱，仅当进入到细胞膜后才可以被激发出很强的荧光。DiO被激发后
可以发出绿色的荧光，DiO和磷酯双层膜结合后的激发光谱和发射光谱参考下图。其中，最大激发波长为484nm，最大发射波长为501nm。
DiO的分子式为C53H85ClN2O6，分子量为881.72，CAS number为34215-57-1。
DiO可以溶解于无水乙醇、DMSO和DMF，溶解度约为1-2.5mg/ml。发现较难溶解时可以适当加热，并
用超声处理以促进溶解。
DiO被广泛用于正向或逆向的，活的或固定的神经等细胞或组织的示踪剂或长期示踪剂(long-term
tracer)。DiO通常不会明显影响细胞的生存力(viability)。DiO对于细胞膜染色的荧光强度通常要低于DiI，有时对于某些经过固定的组织的染色效果欠佳。
DiO除了最简单的细胞膜荧光标记外，还可以用于检测细胞的融合和粘附，检测发育或移植过程中细胞迁移，通过FRAP(Fluorescence Recovery After Photobleaching)检测脂在细胞膜上的扩散，检测细胞毒性和标记脂蛋白等。
用于细胞膜荧光标记时，DiO的常用浓度为1-30μM，最常用的浓度为5-10μM。DiO可以直接染色活
的细胞或组织，染色时间通常为5-20分钟。对于固定的细胞或组织，通常宜使用配制在PBS中的4%多聚甲醛进行固定，使用其它不适当的固定液会导致荧光背景较高。
包装清单：
产品编号 |
产品名称 |
包装 |
C1038 |
DiO(细胞膜绿色荧光探针) |
10mg |
— |
说明书 |
1份 |
保存条件：
4℃避光保存，一年有效。配制的储存液-20℃避光保存，半年有效。
注意事项：
荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明
使用说明：
产品图片
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