产品简介:
ER-Tracker Red是一种内质网(endoplasmic reticulum,ER)红色荧光探针,可以用于活细胞内质网特异性荧光染色。
ER-Tracker Red为采用Molecular Probes公司的BODIPY TR进行了荧光标记的glibenclamide,分子量为915.23。Glibenclamide即glyburide,中文名为格列苯脲,是一种2型糖尿病治疗药物,可以结合主要定位在内质网上的sulphonylurea受体。因此荧光标记的glibenclamide就可以用作内质网特异性的荧光探针。
ER-Tracker Red对于细胞的毒性极低。而传统的DiOC6(3)对ER染色的同时也对细胞有一定的毒性。
ER-Tracker Red呈红色荧光,检测时的最大激发波长为587nm,最大发射波长为615nm。ER-Tracker Red按照后附的使用说明染色后,用甲醛等固定后染色效果可以被部分保留。
提供了ER-Tracker Red 稀释液,使ER-Tracker Red的使用更加便捷。
按照1:1000的比例稀释,可以配制20ml ER-Tracker Red工作液;按照1:3000的比例稀释,可以配制60ml ER-Tracker Red工作液。
包装清单:
产品编号 |
产品名称 |
包装 |
C1041-1 |
ER-Tracker Red(1mM) |
20μl |
C1041-2 |
ER-Tracker Red稀释液 |
60ml |
- |
说明书 |
1份 |
保存条件:
-20℃保存,半年有效。ER-Tracker Red需-20℃避光保存。
注意事项:
ER-Tracker Red(1mM)在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内,可以20-25℃水浴温育片刻至全部融解后使用。对于微量的液体,每次使用前先离心数秒钟,使液体充分沉降到管底。
荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。
需自备盖玻片和载玻片(可以向碧云天订购)。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
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