使用说明：
1. Lyso-Tracker Red工作液的配制：
   a. 取少量Lyso-Tracker Red按照1:13333-1:20000的比例加入到细胞培养液或适当的溶液(例如含钙镁离
      子的HBSS)中，使最终浓度为50-75nM。例如取1μl Lyso-Tracker Red加入到20ml或13.33ml细胞培养
      液或适当的溶液(例如含钙镁离子的HBSS)中。混匀后即为Lyso-Tracker Red工作液。HBSS with Ca²⁺
      & Mg²⁺ (C0219)可以向碧云天订购。
   b. Lyso-Tracker Red工作液使用前需37℃预温育。
      注：工作液中Lyso-Tracker Red的浓度可以根据实际情况进行适当调整。为降低背景，在染色效果可
      以接受的范围内，建议尽量使用较低浓度的Lyso-Tracker Red。
2. 溶酶体的荧光标记：
   a. 去除细胞培养液，加入步骤1配制好的并37℃预温育的Lyso-Tracker Red染色工作液，与细胞37℃共
      孵育30-120分钟。
   b. 去除Lyso-Tracker Red染色工作液，加入新鲜的细胞培养液。
   c. 随后通常用荧光显微镜或激光共聚焦显微镜进行观察。此时可观察到溶酶体呈明亮的强荧光染色。如
      果染色效果欠佳，可以提高Lyso-Tracker Red染色工作液中Lyso-Tracker Red的浓度或在推荐的时间
      范围内适当延长染色时间。

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