使用说明：
1. Caspase抑制剂Z-VAD-FMK在终浓度为20μM时可以非常显著地抑制大多数细胞凋亡诱导剂诱导的细胞凋
   亡。Caspase抑制剂Z-VAD-FMK抑制细胞凋亡的常见有效浓度范围为5-100μM。
2. Caspase抑制剂Z-VAD-FMK用于抑制细胞凋亡时至少需要和细胞凋亡诱导剂同时加入，或者需在细胞凋亡
   诱导剂加入前10-30分钟加入到细胞培养液中，或参考相关文献进行。
3. 如果用于抑制纯化或粗抽提的Caspase，推荐Caspase抑制剂Z-VAD-FMK与待测样品预孵育10-20分钟，再
   进行后续测定。

使用本产品的文献：
1. Deng T, Zhang Y.
   Possible involvement of activation of P53/P21 and demethylation of RUNX 3 in the
   cytotoxicity against Lovo cells induced by 5-Aza-2'-deoxycytidine.
   Life Sci. 2009 Feb 27;84(9-10):311-20. Epub 2008 Dec 31.
2. Deng T, Zhang Y.
   5-Aza-2'-deoxycytidine reactivates expression of RUNX3 by deletion of DNA
   methyltransferases leading to caspase independent apoptosis in colorectal cancer Lovo
   cells.
   Biomed Pharmacother. 2009 Aug;63(7):492-500. Epub 2008 Sep 17.
3. Zhang J, Yan H, Wu YP, Li C, Zhang GY.
   Activation of GluR6-containing kainate receptors induces ubiquitin-dependent Bcl-2
   degradationvia denitrosylation in the rat hippocampus after kainate treatment.
   J Biol Chem. 2011 Mar 4;286(9):7669-80.
4. PengYB, ZhouP, Chu C, Liu EH,et al
   Proteomic Analysis for Malonylastragaloside I in U937 Leukemia Cells by Modified
   Label-free Quantitative Strategy with LC Chip Q-TOF MS/MS
   Chinese Journal of Natural Medicines,2011 Jul, 9(4),305-316
5. Sun X, Huo X, Luo T, Li M, Yin Y, Jiang Y.
   The anticancer flavonoid chrysin induces the unfolded protein response in hepatoma
   cells.
   J Cell Mol Med. 2011 Nov;15(11):2389-98
6. Zhang Y, Zeng F, Liu X, Li Y, Zhou J, Huang Y, Wang Y, Zhou S, Zhu W, Shu E, Zhou G,
   Chen G
   Chan-Yu-Bao-Yuan-Tang induces apoptosis in NSCLC and SCLC cell lines via a
   mitochondria-mediated pathway.
   Xenobiotica. 2011 Jul;41(7):593-602.
7. Wang D, Fang L, Li K, Zhong H, Fan J, Ouyang C, Zhang H, Duan E, Luo R, Zhang Z,
   Liu X, Chen H, Xiao S
   Foot-and-mouth disease virus 3C protease cleaves NEMO to impair innate immune signaling.
   J Virol.2012 Sep;86(17):9311-22. doi: 10.1128/JVI.00722-12. Epub 2012 Jun 20.
8. Mao ZG, Zhou J, Wang H, He DS, Xiao WW, Liao GZ, Qiu LB, Zhu YH, Wang HJ.
   Artesunate inhibits cell proliferation and decreases growth hormone synthesis and
   secretion inGH3 cells.
   Mol Biol Rep. 2012 May;39(5):6227-34. Epub 2012 Jan 5.
9.Song J, Shu L, Zhang Z, Tan X, Sun E, Jin X, Chen Y, Jia X.
   Reactive oxygen species-mediated mitochondrial pathway is involved in Baohuoside
   I-inducedapoptosis in human non-small cell lung cancer.
   Chem Biol Interact. 2012 Jun 9;199(1):9-17.
10.Tang W, Zhang Y, Qian C, Yuan Z, Du J.
   Induction and mechanism of apoptosis by hydroxycamptothecin in human Tenon's
   capsulefibroblasts.
   Invest Ophthalmol Vis Sci. 2012 Jul 24;53(8):4874-80. doi: 10.1167/iovs.11-8968.
11.Khan M, Zheng B, Yi F, Rasul A, Gu Z, Li T, Gao H, Qazi JI, Yang H, Ma T
   Pseudolaric Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis in
   U87Glioblastoma Cells.
   Evid Based Complement Alternat Med. 2012;2012:957568. doi: 10.1155/2012/957568.
   Epub 2012 Jun 20.
12.Zhu L, Yuan H, Guo C, Lu Y, Deng S, Yang Y, Wei Q, Wen L, He Z.
   Zearalenone induces apoptosis and necrosis in porcine granulosa cells via a
   caspase-3- andcaspase-9-dependent mitochondrial signaling pathway.
   J Cell Physiol. 2012 May;227(5):1814-20. doi: 10.1002/jcp.22906.
13.Wang YQ, Wang L, Zhang MY, Wang T, Bao HJ, Liu WL, Dai DK, Zhang L, Chang P, Dong WW,
   Chen XP, Tao LY.
   Necrostatin-1 Suppresses Autophagy and Apoptosis in Mice Traumatic Brain Injury Model.
   Neurochem Res. 2012 Sep;37(9):1849-58. doi: 10.1007/s11064-012-0791-4.
   Epub 2012 Jun 27.
14.Zhao WH, Gou BD, Zhang TL, Wang K.
   Lanthanum chloride bidirectionally influences calcification in bovine vascular smooth
   muscle cells.
   J Cell Biochem. 2012 May;113(5):1776-86. doi: 10.1002/jcb.24049.
15.Chen FH, Lu N, Zhang HW, Zhao L, He LC, Sun HP, You QD, Li ZY, Guo QL.
   LYG-202 augments tumor necrosis factor-α-induced apoptosis via attenuating casein
   kinase 2-dependent nuclearfactor-κB pathway in HepG2 cells.
   Mol Pharmacol. 2012 Nov;82(5):958-71. doi: 10.1124/mol.112.079848. Epub 2012 Aug 21.
16.Wang C , Zhang J, Wang Z, Razzaq M, Khan M.
   Vitexicarpin induces apoptosis-independent mitotic arrest in U87 glioblastoma cells.
   African Journal of Pharmacy and Pharmacology.Vol.6(25),pp.1874-1882,8 July,2012.
17.Shen XJ, Wang HB, Ma XQ, Chen JH.
   β,β-Dimethylacrylshikonin induces mitochondria dependent apoptosis through ERK pathway
   in human gastriccancer SGC-7901 cells.
   PLoS One.2012;7(7):e41773.doi: 10.1371/journal.pone.0041773.Epub 2012 Jul 27.
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   Honokiol induces caspase-independent paraptosis via reactive oxygen species production
   that is accompaniedby apoptosis in leukemia cells.
   Biochem Biophys Res Commun. 2013 Jan 18;430(3):876-82.
   doi:10.1016/j.bbrc.2012.12.063. Epub 2012 Dec 21.
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   Fluopsin C induces oncosis of human breast adenocarcinoma cells.
   Acta Pharmacol Sin.2013 May 27.doi: 10.1038/aps.2013.44.[Epub ahead of print].
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   A Novel Benzothiazole Derivative YLT322 Induces Apoptosis via the Mitochondrial
   Apoptosis Pathway In Vitrowith Anti-Tumor Activity in Solid Malignancies.
   PLoS One.2013 May 30;8(5):e63900.doi: 10.1371/journal.pone.0063900.Print 2013.
21.Sun B, Cai Y, Li Y, Li J, Liu K, Li Y, Yang Y.
   The nonstructural protein NP1 of human bocavirus 1 induces cell cycle arrest and
   apoptosis in Hela cells.
   Virology.2013 May 25;440(1):75-83.doi: 10.1016/j.virol.2013.02.013.Epub 2013 Mar 16.
22.Li Y, Zhu H, Zeng X, Fan J, Qian X, Wang S, Wang Z, Sun Y, Wang X, Wang W, Ju D.
   Suppression of Autophagy Enhanced Growth Inhibition and Apoptosis of Interferon-β in
   Human Glioma Cells.
   Mol Neurobiol.2013 Jun;47(3):1000-10.doi: 10.1007/s12035-013-8403-0.Epub 2013 Jan 18.
23.Yang X, Ouyang H, Chen F, Pang D, Dong M, Yang S, Liu X, Peng Z, Wang F, Zhang X, Ren L.
   HMG-CoA reductase is negatively associated with PCV2 infection and PCV2-induced
   apoptotic cell death.
   J Gen Virol. 2014 Jun;95(Pt 6):1330-7. doi: 10.1099/vir.0.063644-0. Epub 2014 Mar 18.
24.Zhang Y, Ren X, Shi M, Jiang Z, Wang H, Su Q, Liu Q, Li G, Jiang G.
   Downregulation of STAT3 and activation of MAPK are involved in the induction of
   apoptosis by HNK in glioblastoma cell line U87.
   Oncol Rep. 2014 Aug 22. doi: 10.3892/or.2014.3434.
25.Deng Q, Zhang Z, Feng X, Li T, Liu N, Lai J, Shuai L, Xiong Q, Fu C, Zou H, Wang Y, Li
   X, Ma K, Bie P.
   TRAIL-secreting mesenchymal stem cells promote apoptosis in heat-shock-treated liver
   cancer cells and inhibit tumor growth in nude mice.
   Gene Ther. 2014 Mar;21(3):317-27. doi: 10.1038/gt.2013.88. Epub 2014 Jan 23.
26.Teng Y, Gao M, Wang J, Kong Q, Hua H, Luo T, Jiang Y.
   Inhibition of eIF2α dephosphorylation enhances TRAIL-induced apoptosis in hepatoma
   cells.
   Cell Death Dis. 2014 Feb 13;5:e1060. doi: 10.1038/cddis.2014.24.
27.Yu X, Wang H, Liu L.
   Two non-exclusive strategies employed to protect Torulopsis glabrata against
   hyperosmotic stress.
   Appl Microbiol Biotechnol. 2014 Apr;98(7):3099-110. doi: 10.1007/s00253-014-5589-7.
   Epub 2014 Feb 25.
28.Gao X, Zhang H, Zhuang W, Yuan G, Sun T, Jiang X, Zhou Z, Yuan H, Zhang Z, Dong H.
   PEDF and PEDF-derived peptide 44mer protect cardiomyocytes against hypoxia-induced
   apoptosis and necroptosis via anti-oxidative effect.
   Sci Rep. 2014 Jul 11;4:5637. doi: 10.1038/srep05637.
29.Pan Q, Huang Y, Chen L, Gu J, Zhou X.
   SMAC-armed vaccinia virus induces both apoptosis and necroptosis and synergizes the
   efficiency of vinblastine in HCC.
   Hum Cell. 2014 Oct;27(4):162-71. doi: 10.1007/s13577-014-0093-z. Epub 2014 Apr 26.
30.Di W, Khan M, Rasul A, Sun M, Sui Y, Zhong L, Yang L, Zhu Q, Feng L, Ma T.
   Isoalantolactone inhibits constitutive NF-κB activation and induces reactive oxygen
   species-mediated apoptosis in osteosarcoma U2OS cells through mitochondrial dysfunction.
   Oncol Rep. 2014 Oct;32(4):1585-93. doi: 10.3892/or.2014.3368. Epub 2014 Jul 30.
31.Li R, Zhang L, Zhang L, Chen D, Tian J, Cao L, Zhang L.
   Capilliposide C derived from Lysimachia capillipes Hemsl inhibits growth of human
   prostate cancer PC3 cells by targeting caspase and MAPK pathways.
   Int Urol Nephrol. 2014 Jul;46(7):1335-44. doi: 10.1007/s11255-013-0641-6. Epub 2014
   Feb 20.
32.Sun M, Zhang N, Wang X, Cai C, Cun J, Li Y, Lv S, Yang Q.
   Nitidine chloride induces apoptosis, cell cycle arrest, and synergistic cytotoxicity
   with doxorubicin in breast cancer cells.
   Tumour Biol. 2014 Jul 17.
33.Zhang Y, Song J, Zhang W, Liang R, Ma Y, Zhang L, Wei X, Ni J, Wang R.
   Functional properties of a novel hybrid antimicrobial peptide NS: potent antitumor
   activity and efficient plasmid delivery.
   J Pept Sci. 2014 Oct;20(10):785-93. doi: 10.1002/psc.2667. Epub 2014 Jun 23.