使用说明：
1. 对于组织切片或细胞样品或膜，在与碱性磷酸酯酶标记的抗体或其它形式的探针孵育后，用适当洗涤液洗涤3-5次，每次3-5分钟。对于检测内源性碱性磷酸酯酶的组织或细胞样品，在适当固定后，也用适当洗涤液洗涤3-5次，每次3-5分钟。
2. 按照如下比例依次加入各溶液，混匀后即配制成BCIP/NBT染色工作液：

3. 最后一次洗涤完毕后，去除洗涤液，加入适量BCIP/NBT染色工作液，确保能充分覆盖样品。
4. 室温避光孵育5-30分钟或更长时间(可长达24小时)，直至显色至预期深浅。
5. 去除BCIP/NBT染色工作液，用蒸馏水洗涤1-2次即可终止显色反应。
6. 对于组织切片或细胞样品，显色反应终止后，如有必要可以用中性红染色液(neutral red staining solution)染色，以便于观察。对于膜，显色反应终止后，可以室温晾干避光保存。

使用本产品的文献：
1. Jiang S, Zu Y, Fu Y, Zhang Y, Efferth T.
   Activation of the mitochondria-driven pathway of apoptosis in human PC-3 prostate cancer
   cells by a novel hydrophilic paclitaxel derivative, 7-xylosyl-10-deacetylpaclitaxel.
   Int J Oncol. 2008 Jul;33(1):103-11.
2. Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Guo CL, Ma QW, Wang L, Zeng F, Zhou Q.
   iPS cells produce viable mice through tetraploid complementation.
   Nature. 2009 Sep 3;461(7260):86-90.
3. Ji YB, Qu ZY, Zou X.
   Juglone-induced apoptosis in human gastric cancer SGC-7901 cells via the mitochondrial
   pathway.
   Exp Toxicol Pathol. 2009 Oct 6.
4. WU T, NAN K, CHEN J, JIN D, JIANG S, ZHAO P, XU J, DU H, ZHANG X, LI J, PEI G.
   A new bone repair scaffold combined with chitosan/hydroxyapatite and sustained releasing
   icariin.
   Chinese Sci Bull. 2009, 54: 2953―2961.
5. Luo M, Liu X, Zu Y, Fu Y, Zhang S, Yao L, Efferth T.
   Cajanol, a novel anticancer agent from Pigeonpea [Cajanus cajan (L.) Millsp.] roots,
   induces apoptosis in human breast cancer cells through a ROS-mediated mitochondrial
   pathway.
   Chem Biol Interact. 2010;188(1):151-60. Epub 2010 Jul 16.
6. Yin Z, Chen X, Chen JL, Shen WL, Hieu Nguyen TM, Gao L, Ouyang HW.
   The regulation of tendon stem cell differentiation by the alignment of nanofibers.
   Biomaterials. 2010;31(8):2163-75. Epub 2009 Dec 7.
7. Ma YY, Sun D, Li J, Yin ZC.
   Transplantation of endothelial progenitor cells alleviates renal interstitial fibrosis
   in a mouse model of unilateral ureteral obstruction.
   Life Sci. 2010;86(21-22):798-807. Epub 2010 Mar 20.
8. You L, Li L, Xu Q, Ren J, Zhang F.
   Postconditioning reduces infarct size and cardiac myocyte apoptosis via the opioid
   receptor and JAK-STAT signaling pathway.
   Mol Biol Rep. 2010. [Epub ahead of print].
9. Zhao R, Yang W, Wang Z, Li G, Qin W, Wang J.
   Treatment of transplanted tumor of lung adenocarcinoma A549 transfected by human
   somatostatinreceptor subtype 2 (hsstr2) gene with 188Re-RC-160.
   Nucl Med Biol. 2010 Nov;37(8):977-87
10.Chen SY, Qin JJ, Wang L, Mu TW, Jin D, Jiang S, Zhao PR, Pei GX.
   Different effects of implanting vascular bundles and sensory nerve tracts on the
   expression ofneuropeptide receptors in tissue-engineered bone in vivo.
   Biomed Mater. 2010 Oct;5(5):055002.
11.Liu H, Yang Y, Xiao J, Lv Y, Liu Y, Yang H, Zhao L
   COX-2-mediated regulation of VEGF-C in association with lymphangiogenesis and lymph
   nodemetastasis in lung cancer.
   Anat Rec (Hoboken). 2010 Nov;293(11):1838-46.
12.Yuan G, Cai C, Dai J, Liu Y, Zhang R, Dai Y, Wen L, Ding Y.
   Progesterone modulates the proliferation and differentiation of human periodontal
   ligament cells.
   Calcif Tissue Int. 2010 Aug;87(2):158-67.
13.Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Wang X, Wang L, Zeng F, Zhou Q
   Viable fertile mice generated from fully pluripotent iPS cells derived from adult
   somatic cells.
   Stem Cell Rev. 2010 Sep;6(3):390-7.
14.Liu D, Zhang R, Yang X, Xu Y, Tang Z, Tian W, Shen Q.
   Expression, purification and characterization of two thermostable endoglucanases cloned
   from alignocellulosic decomposing fungi Aspergillus fumigatus Z5 isolated from compost.
   Protein Expr Purif. 2011 Oct;79(2):176-86.
15.An XF, Zhou L, Jiang PJ, Yan M, Huang YJ, Zhang SN, Niu YF, Ten SC, Yu JY
   Advanced glycation end-products induce heparanase expression in endothelial cells by
   thereceptor for advanced glycation end products and through activation of the FOXO4
   transcription factor.
   Mol Cell Biochem. 2011 Aug;354(1-2):47-55.
16.Sun D, Liu CX, Ma YY, Zhang L
   Protective effect of prostaglandin E1 on renal microvascular injury in rats of acute
   aristolochic acidnephropathy.
   Ren Fail. 2011;33(2):225-32.
17.Gu J, Sun X, Wang G, Li M, Chi M.
   Icariside II enhances Nrf2 nuclear translocation to upregulate phase II detoxifying
   enzymeexpression coupled with the ERK, Akt and JNK signaling pathways.
   Molecules. 2011 Nov 3;16(11):9234-44.
18.Yu H, Zhang T, Cai L, Qu Y, Hu S, Dong G, Guan R, Xu X, Xing L
   Chamaejasmine induces apoptosis in human lung adenocarcinoma A549 cells through a
   Ros-mediated mitochondrial pathway.
   Molecules. 2011 Sep 27;16(10):8165-80
19.Wang Y, Zhao Y, Liu Y, Tian L, Jin D
   Chamaejasmine inactivates Akt to trigger apoptosis in human HEp-2 larynx carcinoma
   cells.
   Molecules. 2011 Sep 27;16(10):8152-64.
20.Fang W, Liu S, Nie Y.
   Anticancer activity of chamaejasmine: effect on tubulin protein.
   Molecules. 2011 Jul 25;16(8):6243-54.
21.Jiang S, Zu Y, Wang Z, Zhang Y, Fu Y.
   Involvement of mitochondrial permeability transition pore opening in
   7-xylosyl-10-deacetylpaclitaxel-induced apoptosis.
   Planta Med. 2011 Jul;77(10):1005-12.
22.Li Q, Jiang C, Zu Y, Song Z, Zhang B, Meng X, Qiu W, Zhang L
   SFE-CO2 extract from Typhonium giganteum Engl. tubers, induces apoptosis in human
   hepatomaSMMC-7721 cells involvement of a ROS-mediated mitochondrial pathway.
   Molecules. 2011 Sep 28;16(10):8228-42.
23.Chen M, Wang X, Ye Z, Zhang Y, Zhou Y, Tan WS
   A modular approach to the engineering of a centimeter-sized bone tissue construct with
   humanamniotic mesenchymal stem cells-laden microcarriers.
   Biomaterials. 2011 Oct;32(30):7532-42.
24.Li Q, Qiu W, Zhu Q, Zu Y, Deng X, Zhao T, Jiang C, Zhang L
   Camptothecin-20(s)-O-[N-(3'α,12'α-dihydroxy-24'-carbonyl-5'β-cholan)]-lysine,
   a novelcamptothecin analogue, induces apoptosis towards hepatocellular carcinoma
   SMMC-7721 cells.
   Molecules. 2011 Sep 13;16(9):7803-14.
25.Zhang LJ, Pan B, Chen B, Zhang XF, Liang GJ, Feng YN, Wang LQ, Ma JM, Li L, Shen W
   Expression and epigenetic dynamics of transcription regulator Lhx8 during mouse
   oogenesis.
   Gene. 2012 Jul 10.
26.Zhou P, Lian HY, Cui W, Wei DL, Li Q, Liu YX, Liu XY, Tan JH.
   Maternal-restraint stress increases oocyte aneuploidy by impairing metaphase I spindle
   assemblyand reducing spindle assembly checkpoint proteins in mice.
   Biol Reprod. 2012 Mar 22;86(3):83.
27.Han D, Liu XY, Jiao GZ, Liang B, He N, Gao WQ, Tan JH.
   Cyclin B1 turnover and the mechanism causing insensitivity of fully grown mouse oocytes
   tocycloheximide inhibition of meiotic resumption.
   Theriogenology. 2012 Jun;77(9):1900-10.
28.Liu D, Zhang R, Yang X, Zhang Z, Song S, Miao Y, Shen Q.
   Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and
   its functional expression in Pichia pastoris X33.
   Microb Cell Fact. 2012 Feb 17;11:25.
29.Sun D, Ma Y, Han H, Yin Z, Liu C, Feng J, Zhou X, Li X, Xiao A, Yu R
   Thrombospondin-1 short hairpin RNA suppresses tubulointerstitial fibrosis in the kidney
   of ureteralobstruction by ameliorating peritubular capillary injury.
   Kidney Blood Press Res. 2012;35(1):35-47.
30.Zhao L, Liu L, Wu Z, Zhang Y, Chu PK.
   Effects of micropitted/nanotubular titania topographies on bone mesenchymal stem
   cellosteogenic differentiation.
   Biomaterials. 2012 Mar;33(9):2629-41.
31.Mao SS, Hua R, Zhao XP, Qin X, Sun ZQ, Zhang Y, Wu YQ, Jia MX, Cao JL, Zhang YM.
   Exogenous Administration of PACAP Alleviates Traumatic Brain Injury in Rats through a
   Mechanism Involving the TLR4/MyD88/NF-κB Pathway.
   J Neurotrauma. 2012 Jul 1;29(10):1941-59.
32.Zhao X, Liu L, Wang FK, Zhao DP, Dai XM, Han XS.
   Coculture of vascular endothelial cells and adipose-derived stem cells as a source for
   boneengineering.
   Ann Plast Surg. 2012 Jul;69(1):91-8.
33.Gu S, He J.
   Daphnoretin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cells.
   Molecules. 2012 Jan 9;17(1):598-612.
34.Zhang L, Zhang L, Han X, Du M, Zhang Y, Feng Z, Yi H, Zhang Y
   Enhancement of transglutaminase production in Streptomyces mobaraensis as achieved
   bytreatment with excessive MgCl2.
   Appl Microbiol Biotechnol. 2012 Mar;93(6):2335-43.
35.Gao Q, Zhao L, Song Z, Yang G
   Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs.
   Mol Biol Rep. 2012 May;39(5):5791-804.
36.Wen L, Wang Y, Wang H, Kong L, Zhang L, Chen X, Ding Y.
   L-type calcium channels play a crucial role in the proliferation and osteogenic
   differentiation ofbone marrow mesenchymal stem cells.
   Biochem Biophys Res Commun. 2012 Jul 3.
37.Tang RX, Kong FY, Fan BF, Liu XM, You HJ, Zhang P, Zheng KY.
   HBx activates FasL and mediates HepG2 cell apoptosis through MLK3-MKK7-JNKs signal
   module.
   World J Gastroenterol. 2012 Apr 7;18(13):1485-95.
38.Liu Y, Xiao Z, Yang D, Ren L, Liu G, Yang L.
   Effects of the cyclin-dependent kinase 10 (CDK10) on the tamoxifen sensitivity of keloid
   samples.
   Molecules. 2012 Feb 1;17(2):1307-18.
39.Na L
   Growth Inhibition and Cell Cycle Arrest of Kappa-Selenocarrageenan and Paclitaxel on
   HepG2 Cells
   Advanced Materials Research, 2012,343-344, 530
40.Luan HF, Zhao ZB, Zhao QH, Zhu P, Xiu MY.
   Hydrogen sulfide postconditioning protects isolated rat hearts against ischemia and
   reperfusioninjury mediated by the JAK2/STAT3 survival pathway.
   Braz J Med Biol Res. 2012 May 31.
41.Hualin C, Wenli X, Dapeng L, Xijing L, Xiuhua P, Qingfeng P.
   The anti-inflammatory mechanism of heme oxygenase-1 induced by hemin in primary rat
   alveolarmacrophages.
   Inflammation. 2012 Jun;35(3):1087-93.
42.Qin X, Sun ZQ, Dai XJ, Mao SS, Zhang JL, Jia MX, Zhang YM.
   Toll-like receptor 4 signaling is involved in PACAP-induced neuroprotection in BV2
   microglial cellsunder OGD/reoxygenation.
   Neurol Res. 2012 May;34(4):379-89.
43.Jiao GZ, Cao XY, Cui W, Lian HY, Miao YL, Wu XF, Han D, Tan JH.
   Developmental potential of prepubertal mouse oocytes is compromised due mainly to their
   impaired synthesis ofglutathione.
   PLoS One. 2013;8(3):e58018. doi: 10.1371/journal.pone.0058018. Epub 2013 Mar 1.
44.Chuan J, Li Y, Yang L, Sun X, Zhang Q, Gong T,Zhang Z.
   Enhanced rifampicin delivery to alveolar macrophages by solid lipid nanoparticles.
   Journal of Nanoparticle Research.April 2013, 15:1634.
45.Li N, Liu N, Zhou J, Tang L, Ding B, Duan Y, Jin Y.
   Inflammatory environment induces gingival tissue-specific mesenchymal stem cells to
   differentiate towards a pro-fibrotic phenotype.
   Biol Cell. 2013 Mar 22. doi: 10.1111/boc.201200064. [Epub ahead of print].
46.Cai C, Yuan GJ, Huang Y, Yang N, Chen X, Wen L, Wang X, Zhang L, Ding Y.
   Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal
   stem cells isolated fromhuman periodontal ligaments.
   Int J Mol Med. 2013 May;31(5):1195-201. doi: 10.3892/ijmm.2013.1305. Epub 2013 Mar 15.
47.Liang L, Luo M, Fu Y, Zu Y, Wang, W, Gu C, Zhao C, Li C, Efferthc T.
   Cajaninstilbene acid (CSA) exerts cytoprotective effects against oxidative stress
   through the Nrf2-dependent antioxidant pathway.
   Toxicology Letters.Volume 219, Issue 3, 7 June 2013, Pages 254–261.
48.Liu H, Peng H, Wu Y, Zhang C, Cai Y, Xu G, Li Q.
   The promotion of bone regeneration by nanofibrous hydroxyapatite/chitosan scaffolds by
   effects on integrin-BMP/Smad signaling pathway in BMSCs.
   Biomaterials.Volume 34, Issue 18, June 2013, Pages 4404–4417.
49.Zhang JK, Yang L, Meng GL, Yuan Z, Fan J, Li D, Chen JZ, Shi TY, Hu HM, Wei BY, Luo ZJ,
   Liu J.
   Protection by salidroside against bone loss via inhibition of oxidative stress and
   bone-resorbing mediators.
   PLoS One. 2013;8(2):e57251. doi: 10.1371/journal.pone.0057251. Epub 2013 Feb 20.
50.Li H, Zhang L, Cui Y, Luo X, Xue C, Wang S.
   Expression of soluble recombinant transglutaminase from Zea mays in Pichia pastoris.
   World J Microbiol Biotechnol.2013 May;29(5):939-47.doi:10.1007/s11274-012-1250-8.
   Epub 2013 Jan 8.
51.Sun Y, Mu F, Li C, Wang W, Luo M, Fu Y, Zu Y.
   Aspidin BB, a phloroglucinol derivative, induces cell cycle arrest and apoptosis in
   human ovarian HO-8910 cells.
   Chem Biol Interact. 2013 Apr 27. pii: S0009-2797(13)00094-X.
   doi: 10.1016/j.cbi.2013.04.008. [Epub ahead of print].
52.Dong YH, Li ZQ, Sun Y, Zhuang L, Wang YK, Sun Q.
   Downregulation of pigment epithelium-derived factor in condyloma acuminatum.
   J Int Med Res. 2013 Mar 14. [Epub ahead of print].
53.Yang Z, Liu J, Liu H, Qiu M, Liu Q, Zheng L, Pang M, Quan F, Zhang Y.
   Isolation and Characterization of SSEA3(+) Stem Cells Derived from Goat Skin
   Fibroblasts.
   Cell Reprogram.2013 Jun;15(3):195-205.doi:10.1089/cell.2012.0080.Epub 2013 May 13.
54.Liang L, Gao C, Luo M, Zhao C, Wang W, Gu C, Yu J, Fu Y.
   The Phytoestrogenic Compound Cajanol from Pigeonpea Roots is Associated with the
   Activation of Estrogen Receptor α-dependent Signaling Pathway in Human Prostate Cancer
   Cells.
   Phytother Res. 2013 Feb 19. doi: 10.1002/ptr.4937. [Epub ahead of print].
55.Qin X, Sun ZQ, Zhang XW, Dai XJ, Mao SS, Zhang YM.
   TLR4 signaling is involved in the protective effect of propofol in BV2 microglia against
   OGD/reoxygenation.
   J Physiol Biochem. 2013 Mar 20. [Epub ahead of print].
56.Wu K, Xu J, Liu M, Song W, Yan J, Gao S, Zhao L, Zhang Y.
   Induction of osteogenic differentiation of stem cells via a lyophilized microRNA reverse
   transfection formulationon a tissue culture plate.
   Int J Nanomedicine. 2013;8:1595-607. doi: 10.2147/IJN.S43244. Epub 2013 May 3.
57.Li Q, Zhao XL, Sun J, Jiang SG, Gong XF.
   Anti-proliferative and apoptosis-inducing activities of juglone in LS-174T cells.
   Bangladesh Journal of Pharmacology. Vol 8, No 1 (2013).
58.Zhang P, Wu Y, Dai Q, Fang B, Jiang L.
   p38-MAPK signaling pathway is not involved in osteogenic differentiation during early
   response of mesenchymal stem cells to continuous mechanical strain.
   Mol Cell Biochem. 2013 Jun;378(1-2):19-28. doi: 10.1007/s11010-013-1589-7.
   Epub 2013 Feb 23.
59.Zhang E, Yan X, Zhang M, Chang X, Bai Z, He Y, Yuan Z.
   Aggrecanases in the human synovial fluid at different stages of osteoarthritis.
   Clin Rheumatol. 2013 Jan 31. [Epub ahead of print].
60.Chen M, Zhou M, Ye Z, Zhou Y, Tan WS.
   Ectopic Osteogenesis of Macroscopic Tissue Constructs Assembled from Human Mesenchymal
   Stem Cell-Laden Microcarriers through In Vitro Perfusion Culture.
   PLoS One. 2014 Oct 2;9(10):e109214. doi: 10.1371/journal.pone.0109214. eCollection
   2014.
61.Lin Z, Feng R, Li J, Meng Y, Yuan L, Fu Z, Guo J, Bringhurst FR, Yang D.
   Nuclear translocation of CBP/p300-interacting protein CITED1 induced by parathyroid
   hormone requires serine phosphorylation at position 79 in its 63-84 domain.
   Cell Signal. 2014 Nov;26(11):2436-45. doi: 10.1016/j.cellsig.2014.06.015. Epub 2014
   Jul 15.
62.Ji CF, Ji YB.
   Apoptosis of human gastric cancer SGC-7901 cells induced by podophyllotoxin.
   Exp Ther Med. 2014 May;7(5):1317-1322. Epub 2014 Mar 6.
63.Shi JX, Li JS, Hu R, Shi Y, Su X, Li Q, Zhang F.
   CNOT7/hCAF1 is involved in ICAM-1 and IL-8 regulation by tristetraprolin.
   Cell Signal. 2014 Nov;26(11):2390-6. doi: 10.1016/j.cellsig.2014.07.020. Epub 2014 Jul
   16.
64.Xu Q, Li B, Yuan L, Dong Z, Zhang H, Wang H, Sun J, Ge S, Jin Y.
   Combination of platelet-rich plasma within periodontal ligament stem cell sheets
   enhances cell differentiation and matrix production.
   J Tissue Eng Regen Med. 2014 Sep 4. doi: 10.1002/term.1953.
65.Xie B, Wang J, Liu S, Wang J, Xue B, Li J, Wei R, Zhao Y, Liu Z.
   Positive correlation between the efficiency of induced pluripotent stem cells and the
   development rate of nuclear transfer embryos when the same porcine embryonic
   fibroblast lines are used as donor cells.
   Cell Reprogram. 2014 Jun;16(3):206-14. doi: 10.1089/cell.2013.0080. Epub 2014 Apr 16.
66.Shi JX, Li JS, Hu R, Shi Y, Su X, Guo XJ, Li XM.
   Tristetraprolin is involved in the glucocorticoid-mediated interleukin 8 repression.
   Int Immunopharmacol. 2014 Oct;22(2):480-5. doi: 10.1016/j.intimp.2014.07.031. Epub
   2014 Aug 8.
67.Yao Y, Shi X, Chen F.
   The effect of gold nanoparticles on the proliferation and differentiation of murine
   osteoblast: a study of MC3T3-E1 cells in vitro.
   J Nanosci Nanotechnol. 2014 Jul;14(7):4851-7.
68.Cao L, Werkmeister JA, Wang J, Glattauer V, McLean KM, Liu C.
   Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N,
   6-O-sulfated chitosan nanoparticles.
   Biomaterials. 2014 Mar;35(9):2730-42. doi: 10.1016/j.biomaterials.2013.12.028. Epub
   2014 Jan 15.
69.Du J, Wu Y, Ai Z, Shi X, Chen L, Guo Z.
   Mechanism of SB431542 in inhibiting mouse embryonic stem cell differentiation.
   Cell Signal. 2014 Oct;26(10):2107-16. doi: 10.1016/j.cellsig.2014.06.002. Epub 2014
   Jun 18.
70.Xie Q, Wang Z, Bi X, Zhou H, Wang Y, Gu P, Fan X.
   Effects of miR-31 on the osteogenesis of human mesenchymal stem cells.
   Biochem Biophys Res Commun. 2014 Mar 28;446(1):98-104. doi: 10.1016/j.bbrc.
   2014.02.058. Epub 2014 Feb 21.
71.Yan J, Zhang C, Zhao Y, Cao C, Wu K, Zhao L, Zhang Y.
   Non-viral oligonucleotide antimiR-138 delivery to mesenchymal stem cell sheets and the
   effect on osteogenesis.
   Biomaterials. 2014 Sep;35(27):7734-49. doi: 10.1016/j.biomaterials.2014.05.089. Epub
   2014 Jun 19.
72.Li P, Wang T, Mao Y, Zhang Y, Niu L, Liang R, Zhu L, Luo X.
   Effect of ultimate pH on postmortem myofibrillar protein degradation and meat quality
   characteristics of Chinese Yellow crossbreed cattle.
   ScientificWorldJournal. 2014;2014:174253. doi: 10.1155/2014/174253. Epub 2014 Aug
   13.
73.Guo D, Li Q, Lv Q, Wei Q, Cao S, Gu J.
   MiR-27a targets sFRP1 in hFOB cells to regulate proliferation, apoptosis and
   differentiation.
   PLoS One. 2014 Mar 13;9(3):e91354. doi: 10.1371/journal.pone.0091354. eCollection
   2014.
74.Wang W, Liu Q, Zhang Y, Zhao L.
   Involvement of ILK/ERK1/2 and ILK/p38 pathways in mediating the enhanced osteoblast
   differentiation by micro/nanotopography.
   Acta Biomater. 2014 Aug;10(8):3705-15. doi: 10.1016/j.actbio.2014.04.019. Epub 2014
   Apr 24.
75.Guo H, Wang Y, Song T, Xin T, Zheng Z, Zhong P, Zhang X.
   Silencing of Survivin Using YM155 Inhibits Invasion and Suppresses Proliferation in
   Glioma Cells.
   Cell Biochem Biophys. 2014 Sep 9.
76.Wang J, An Y, Li F, Li D, Jing D, Guo T, Luo E, Ma C.
   The effects of pulsed electromagnetic field on the functions of osteoblasts on implant
   surfaces with different topographies.
   Acta Biomater. 2014 Feb;10(2):975-85. doi: 10.1016/j.actbio.2013.10.008. Epub 2013 Oct
   17.
77.Chen Y, Zheng L, Liu J, Zhou Z, Cao X, Lv X, Chen F.
   Shikonin inhibits prostate cancer cells metastasis by reducing matrix metalloproteinase-
   2/-9 expression via AKT/mTOR and ROS/ERK1/2 pathways.
   Int Immunopharmacol. 2014 Aug;21(2):447-55. doi: 10.1016/j.intimp.2014.05.026. Epub
   2014 Jun 3.
78.Liu Y, Liu Y, Zhang R, Wang X, Huang F, Yan Z, Nie M, Huang J, Wang Y, Wang Y, Chen L,
   Yin L, He B, Deng Z.
   All-trans retinoic acid modulates bone morphogenic protein 9-induced osteogenesis and
   adipogenesis of preadipocytes through BMP/Smad and Wnt/β-catenin signaling pathways.
   Int J Biochem Cell Biol. 2014 Feb;47:47-56. doi: 10.1016/j.biocel.2013.11.018. Epub
   2013 Dec 1.
79.Xiao J, Gao H, Jin Y, Zhao Z, Guo J, Liu Z, Zhao Z.
   The abnormal expressions of tristetraprolin and the VEGF family in uraemic rats with
   peritoneal dialysis.
   Mol Cell Biochem. 2014 Jul;392(1-2):229-38. doi: 10.1007/s11010-014-2033-3. Epub 2014
   Apr 3.
80.Wang C, Lin K, Chang J, Sun J.
   The stimulation of osteogenic differentiation of mesenchymal stem cells and vascular
   endothelial growth factor secretion of endothelial cells by β-CaSiO3/β-Ca3(PO4)2
   scaffolds.
   J Biomed Mater Res A. 2014 Jul;102(7):2096-104. doi: 10.1002/jbm.a.34880. Epub 2013
   Aug 2.
81.Yang M, Shuai Y, Zhang C, Chen Y, Zhu L, Mao C, OuYang H.
   Biomimetic nucleation of hydroxyapatite crystals mediated by Antheraea pernyi silk
   sericin promotes osteogenic differentiation of human bone marrow derived mesenchymal
   stem cells.
   Biomacromolecules. 2014 Apr 14;15(4):1185-93. doi: 10.1021/bm401740x. Epub 2014 Mar
   26.