使用说明： 1. 裂解细胞：将报告基因细胞裂解液充分混匀后，按如下方式加入报告基因细胞裂解液，充分裂解细    胞。    a. 对于贴壁细胞：吸尽细胞培养液后，参考下表加入适量的报告基因细胞裂解液；对于悬浮细胞：       离心去上清后，参考下表加入适量报告基因细胞裂解液。

器皿类型 96孔板 48孔板 24孔板 12孔板 6孔板 报告基因细胞裂解液 (微升/孔) 100 150 200 300 500

注：如果荧光素酶的表达水平比较低，可以尝试使用更少的裂解液，例如6孔板的每孔用量可以最小    为100微升。    b. 充分裂解后，10,000-15,000g离心3-5分钟，取上清用于测定。    注：细胞裂解后可立即测定萤光素酶，也可以先冻存，待以后再测定。冻存样品需融解，并达到室温    后再进行测定。 2. 融解萤光素酶检测试剂，并达到室温。 3. 按仪器操作说明书开启化学发光仪或具有检测化学发光功能的多功能酶标仪，将测定间隔设为2秒，    测定时间设为10秒。 4. 每个样品测定时，取样品20-100微升(如果样品量足够，请加入100微升；如果样品量不足可以适当减    少用量，但同批样品的使用量宜保持一致)。 5. 加入100微升萤光素酶检测试剂，用枪打匀或用其它适当方式混匀后测定RLU(relative light    unit)。以报告基因细胞裂解液为空白对照。本试剂盒的检测效果以及与同类竞争产品的检测效果比    较可以参考图2。                    图2. 本产品和P公司竞争产品(Competitor P)对相同样品的检测效果。实际读数会因细胞数量、转染效果、检测仪器等的不同而存在差异，图中数据仅供参考。

常见问题：
1. Luminometer和荧光分光光度计有何不同？
    荧光分光光度计检测的样品本身不能发光，样品需要由特定波长的激发光激发，然后才能产生荧光
    并被荧光分光光度计检测。Luminometer检测的样品本身可以发光，不需要激发光进行激发。也就是
    说luminometer是检测化学发光(萤光)的仪器。有些型号的荧光分光光度计也具有luminometer的功
    能，即也可以检测化学发光。您所使用的荧光分光光度计能否用于化学发光的测定请仔细阅读该仪
    器的说明书。
2. 可以进行ATP化学发光检测的仪器是否就可以用于本试剂盒的检测？
    是。ATP化学发光的检测原理和本试剂盒的原理相同，可以用相同的仪器测定。

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