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BCECF AM(pH荧光探针) |
产品编号: S1006
产品包装: 50微升
产品价格: 430.00元 |
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产品简介
产品简介:
BCECF AM,即2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein,acetoxymethyl
ester,是最常用的检测细胞内pH的荧光探针。
本BCECF AM(pH荧光探针)是配制于无水DMSO(anhydrous DMSO)中的储存液,浓度为5mM。
BCECF AM是一种可以穿透细胞膜的荧光染料。BCECF AM没有荧光,进入细胞后可以被细胞内的酯酶
剪切形成BCECF,从而被滞留在细胞内。BCECF在适当的pH值情况下可以被激发形成绿色荧光。最大激发波长和发射波长因pH的不同而有所不同,最大激发波长大致在503nm左右,最大发射波长大致在520nm左右,实际检测时推荐使用的激发波长为488nm,发射波长为535nm。BCECF在不同pH条件下的发射光谱参考下图。
BCECF AM不仅被广泛用于哺乳动物细胞的研究,也有报道用于动物组织、植物细胞、细菌和酵母等
的细胞内pH水平检测。在有细胞内pH变化的细胞毒性、细胞凋亡、细胞粘附、药物抵抗、细胞趋化等过程中BCECF AM被广泛应用。
用于细胞内pH检测时,常用的BCECF AM的浓度为1-10μM。
包装清单:
产品编号 |
产品名称 |
包装 |
S1006 |
BCECF AM(pH荧光探针, 5mM) |
50微升 |
— |
说明书 |
1份 |
保存条件:
-20℃避光保存,一年有效。
注意事项:
BCECF AM(pH荧光探针, 5mM)可能对人体有害,请注意适当防护。
本BCECF AM在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内,可以20-25℃
水浴温育片刻至全部融解后使用。
荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明
产品图片
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