使用说明：
1. 在室温或不超过37℃的水浴中溶解SDS-PAGE上样缓冲液(5X)。水浴溶解后立即室温存放，尽量避免长时
   间置于水浴中。
2. 按照每4微升蛋白样品加入1微升蛋白上样缓冲液(5X)的比例，混合蛋白样品和蛋白上样缓冲液(5X)。
3. 100℃或沸水浴加热3-5分钟，以充分变性蛋白。
4. 冷却到室温后，直接上样到SDS-PAGE胶加样孔内即可。
5. 通常电泳至蓝色染料到达胶的底端处附近即可停止电泳。

使用本产品的相关论文：
1. Cai H, Yin D, Zhang L, Yang X, Xu X, Liu W, Zheng X, Zhang H, Wang J, Xu Y, Cheng D,
   Zheng M, Han Y, Wu M, Wang Y.
   Preparation and biological evaluation of 2-amino-6-[18F]fluoro-9-(4-hydroxy-3-hydroxy-
   methylbutyl) purine (6-[18F]FPCV) as a novel PET probe for imaging HSV1-tk reporter gene
   expression.
   Nucl Med Biol. 2007 Aug;34(6):717-25.
2. Yuan Q, Li PD, Li BH, Yang XZ, Xu SB, Liu XH, Zhou FX, Zhang WJ.
   Differential IL-4/Stat6 activities correlate with differential expression of regulatory
   genes SOCS-1, SHP-1, and PP2A in colon cancer cells.
   J Cancer Res Clin Oncol. 2008 Jun 7.
3. Li H, Zhang L, Huang Q.
   Differential expression of mitogen-activated protein kinase signaling pathway in the
   hippocampus of rats exposed to chronic unpredictable stress.
   Behav Brain Res. 2009 Dec 14;205(1):32-7. Epub 2009 Jul 1.
4. Cao BY, Yang YP, Luo WF, Mao CJ, Han R, Sun X, Cheng J, Liu CF.
   Paeoniflorin, a potent natural compound, protects PC12 cells from MPP+ and acidic damage
   via autophagic pathway.
   J Ethnopharmacol. 2010;131(1):122-9. Epub 2010 Jun 15.
5. Xie Y, Hao H, Kang A, Liang Y, Xie T, Sun S, Dai C, Zheng X, Xie L, Li J, Wang G.
   Integral pharmacokinetics of multiple lignan components in normal, CCl4-induced hepatic
   injuryand hepatoprotective agents pretreated rats and correlations with hepatic injury
   biomarkers.
   J Ethnopharmacol. 2010 Sep 15;131(2):290-9.
6. Tang WJ, Wang LF, Xu XY, Zhou Y, Jin WF, Wang HF, Gao J.
   Autocrine/paracrine action of vitamin D on FGF23 expression in cultured rat osteoblasts.
   Calcif Tissue Int. 2010 May;86(5):404-10.
7. Tang DQ, Wei YQ, Yin XX, Lu Q, Hao HH, Zhai YP, Wang JY, Ren J.
   In vitro suppression of quercetin on hypertrophy and extracellular matrix accumulation
   in ratglomerular mesangial cells cultured by high glucose.
   Fitoterapia. 2011 Sep;82(6):920-6. Epub 2011 May 14.
8. Wang D, Fang L, Li P, Sun L, Fan J, Zhang Q, Luo R, Liu X, Li K, Chen H, Chen Z, Xiao S.
   The leader proteinase of foot-and-mouth disease virus negatively regulates the type I
   interferonpathway by acting as a viral deubiquitinase.
   J Virol. 2011 Apr;85(8):3758-66.
9. Tang DQ, Wei YQ, Gao YY, Yin XX, Yang DZ, Mou J, Jiang XL.
   Protective effects of rutin on rat glomerular mesangial cells cultured in high glucose
   conditions.
   Phytother Res. 2011 Nov;25(11):1640-7.
10.Liu M, Li ZH, Xu FJ, Lai LH, Wang QQ, Tang GP, Yang WT
   An oligopeptide ligand-mediated therapeutic gene nanocomplex for liver cancer-targeted
   therapy.
   Biomaterials. 2012 Mar;33(7):2240-50.
11.Lu X, Zhang N, Dong S, Hu Y.
   Involvement of GPR12 in the induction of neurite outgrowth in PC12 cells.
   Brain Res Bull. 2012 Jan 4;87(1):30-6. Epub 2011 Oct 1
12.Mao SS, Hua R, Zhao XP, Qin X, Sun ZQ, Zhang Y, Wu YQ, Jia MX, Cao JL, Zhang YM.
   Exogenous Administration of PACAP Alleviates Traumatic Brain Injury in Rats through a
   Mechanism Involving the TLR4/MyD88/NF-κB Pathway.
   J Neurotrauma. 2012 Jul 1;29(10):1941-59.
13.Jia P, Xu YJ, Zhang ZL, Li K, Li B, Zhang W, Yang H.
   Ferric ion could facilitate osteoclast differentiation and bone resorption through the
   production ofreactive oxygen species.
   J Orthop Res. 2012 May 8. doi: 10.1002/jor.22133.
14.Xiang Y, Zheng K, Ju H, Wang S, Pei Y, Ding W, Chen Z, Wang Q, Qiu X, Zhong M, Zeng F,
   Ren Z, Qian C, Liu G, Kitazato K, Wang Y.
   Cofilin 1-mediated biphasic f-actin dynamics of neuronal cells affect herpes simplex
   virus 1infection and replication.
   J Virol. 2012 Aug;86(16):8440-51.
15.Xie Y, Wang G, Wang H, Yao X, Jiang S, Kang A, Zhou F, Xie T, Hao H
   Cytochrome P450 dysregulations in thioacetamide-induced liver cirrhosis in rats and
   thecounteracting effects of hepatoprotective agents.
   Drug Metab Dispos. 2012 Apr;40(4):796-802.
16.Yang J, Zhang J, Chen W, Hu Z, Zhu J, Fang X, Yuan W, Li M, Hu X, Tan Y, Hu F, Rao X
   Eliciting cross-neutralizing antibodies in mice challenged with a dengue virus envelope
   domain IIIexpressed in Escherichia coli
   Can J Microbiol. 2012 Apr;58(4):369-80. Epub 2012 Mar 15
17.Luan HF, Zhao ZB, Zhao QH, Zhu P, Xiu MY
   Hydrogen sulfide postconditioning protects isolated rat hearts against ischemia and
   reperfusioninjury mediated by the JAK2/STAT3 survival pathway.
   Braz J Med Biol Res. 2012 May 31.
18.Ye HH, Wu KJ, Fei SJ, Zhang XW, Liu HX, Zhang JL, Zhang YM.
   Propofol participates in gastric mucosal protection through inhibiting the toll-like
   receptor-4/nuclear factor kappa-B signaling pathway.
   Clin Res Hepatol Gastroenterol. 2013 Feb;37(1):e3-15.
   doi:10.1016/j.clinre.2012.03.004.Epub 2012 Apr 17.
19.Xiang Q, Wang Y, Wu W, Meng X, Qiao Y, Xu L, Liu X.
   Carnosic acid protects against ROS/RNS-induced protein damage and upregulates HO-1
   expression in RAW264. 7 macrophages.
   Journal of Functional Foods.Volume 5, Issue 1, January 2013, Pages 362–369.
20.Qin X, Sun ZQ, Zhang XW, Dai XJ, Mao SS, Zhang YM.
   TLR4 signaling is involved in the protective effect of propofol in BV2 microglia against
   OGD/reoxygenation.
   J Physiol Biochem. 2013 Mar 20.
21.Jiao Q, Wang H, Hu Z, Zhuang Y, Yang W, Li M, Yu X, Liang J, Guo Y, Zhang H, Chen X,
   Cheng R, Yao Z.
   Lidocaine inhibits staphylococcal enterotoxin-stimulated activation of peripheral blood
   mononuclear cells frompatients with atopic dermatitis.
   Arch Dermatol Res. 2013 Apr 16.
22.Gao YD, Zheng JW, Li P, Cheng M, Yang J.
   Store-operated Ca²⁺ entry is involved in transforming growth factor-β1 facilitated
   proliferation of rat airwaysmooth muscle cells.
   J Asthma. 2013 Jun;50(5):439-48. doi: 10.3109/02770903.2013.778275. Epub 2013 May 7.
23.Ye HH, Hua R, Yu L, Wu KJ, Fei SJ, Qin X, Song Y, Cao JL, Zhang YM.
   Abnormal Expression of Toll-Like Receptor 4 Is Associated with Susceptibility to
   Ethanol-Induced GastricMucosal Injury in Mice.
   Dig Dis Sci. 2013 Jun 7.
24.You H, Li T, Zhang J, Lei Q, Tao X, Xie P, Lu W.
   Reduction in ischemic cerebral infarction is mediated through golgi phosphoprotein 3 and
   Akt/mTOR signaling following salvianolate administration.
   Curr Neurovasc Res. 2014 May;11(2):107-13.
25.Ji CF, Ji YB.
   Apoptosis of human gastric cancer SGC-7901 cells induced by podophyllotoxin.
   Exp Ther Med. 2014 May;7(5):1317-1322. Epub 2014 Mar 6.
26.Ge XY, Yang LQ, Jiang Y, Yang WW, Fu J, Li SL.
   Reactive oxygen species and autophagy associated apoptosis and limitation of clonogenic
   survival induced by zoledronic acid in salivary adenoid cystic carcinoma cell line
   SACC-83.
   PLoS One. 2014 Jun 25;9(6):e101207. doi: 10.1371/journal.pone.0101207. eCollection
   2014.
27.Li Y, Shen D, Tang X, Li X, Wo D, Yan H, Song R, Feng J, Li P, Zhang J, Li J.
   Chlorogenic acid prevents isoproterenol-induced hypertrophy in neonatal rat myocytes.
   Toxicol Lett. 2014 May 2;226(3):257-63. doi: 10.1016/j.toxlet.2014.02.016. Epub 2014
   Feb 28.
28.Liang C, Guo S, Yang L.
   Effects of all-trans retinoic acid on VEGF and HIF-1α expression in glioma cells under
   normoxia and hypoxia and its anti-angiogenic effect in an intracerebral glioma model.
   Mol Med Rep. 2014 Nov;10(5):2713-9. doi: 10.3892/mmr.2014.2543. Epub 2014 Sep 5.
29.He X, Song W, Liu C, Chen S, Hua J.
   Rapamycin inhibits acrolein-induced apoptosis by alleviating ROS-driven mitochondrial
   dysfunction in male germ cells.
   Cell Prolif. 2014 Apr;47(2):161-71. doi: 10.1111/cpr.12091. Epub 2014 Jan 31.
30.Mao H, Wang H, Ma S, Xu Y, Zhang H, Wang Y, Niu Z, Fan G, Zhu Y, Gao XM.
   Bidirectional regulation of bakuchiol, an estrogenic-like compound, on catecholamine
   secretion.
   Toxicol Appl Pharmacol. 2014 Jan 1;274(1):180-9. doi: 10.1016/j.taap.2013.11.001.
   Epub 2013 Nov 11.
31.Xia Y, Qi F, Zou J, Yang J, Yao Z.
   Influenza vaccination during early pregnancy contributes to neurogenesis and behavioral
   function in offspring.
   Brain Behav Immun. 2014 Jul 8. pii: S0889-1591(14)00387-0. doi: 10.1016/j.bbi.
   2014.06.202.
32.Xin F, Jiang L, Liu X, Geng C, Wang W, Zhong L, Yang G, Chen M.
   Bisphenol A induces oxidative stress-associated DNA damage in INS-1 cells.
   Mutat Res Genet Toxicol Environ Mutagen. 2014 Jul 15;769:29-33. doi: 10.1016/
   j.mrgentox.2014.04.019. Epub 2014 May 21.
33.Yang Y, Hou J, Lin Z, Zhuo H, Chen D, Zhang X, Chen Y, Sun B.
   Attenuated Listeria monocytogenes as a cancer vaccine vector for the delivery of CD24, a
   biomarker for hepatic cancer stem cells.
   Cell Mol Immunol. 2014 Mar;11(2):184-96. doi: 10.1038/cmi.2013.64. Epub 2014 Feb 3.
34.Zhang S, Sun Y, Chen H, Dai Y, Zhan Y, Yu S, Qiu X, Tan L, Song C, Ding C.
   Activation of the PKR/eIF2α signaling cascade inhibits replication of Newcastle disease
   virus.
   Virol J. 2014 Mar 31;11:62. doi: 10.1186/1743-422X-11-62.