胰酶细胞消化液

 产品编号: C0201
 产品包装: 100ml
 产品价格: 28.00元
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产品简介

产品简介:

产品编号 产品名称 产品包装 产品价格
C0201 胰酶细胞消化液 100ml 28.00元

    碧云天生产的胰酶细胞消化液(Trypsin-EDTA Solution)含0.25%胰酶和0.02%EDTA。该消化液经过过滤
除菌,可以直接用于培养细胞的消化,或者一些组织的消化。
    本胰酶细胞消化液具有方便快速的特点,通常室温消化1分钟左右就可以消化下大多数贴壁细胞。
包装清单:

产品编号

产品名称

包装

C0201

胰酶细胞消化液

100ml

说明书

1份

保存条件:
    4℃保存,一年有效。
注意事项:
    在使用胰酶细胞消化液的过程中要特别注意避免消化液被细菌污染。
    胰酶细胞消化液消化细胞时间不宜过长,否则细胞铺板后生长状况会较差。
    为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明

使用说明:
1. 贴壁细胞的消化:
   a) 吸去培养液,用无菌的PBS、Hanks液或无血清培养液洗涤细胞一次,以去除残余的血清。
   b) 加入少量胰酶细胞消化液,略盖过细胞即可,室温放置30秒至2分钟。不同的细胞消化时间有所不
      同。
   c) 显微镜下观察,细胞明显收缩,并且肉眼观察培养器皿底部发现细胞的形态发生明显的变化;或者用
      枪吹打细胞发现细胞刚好可以被吹打下来。此时吸除胰酶细胞消化液。加入含血清的完全细胞培养
      液,吹打下细胞,即可直接用于后续实验。
   d) 如果发现消化不足,则加入胰酶细胞消化液重新消化。
   e) 如果发现细胞消化时间过长,未及吹打细胞,细胞已经有部分直接从培养器皿底部脱落,直接用胰酶
      细胞培养液把细胞全部吹打下来。1000-2000g离心1分钟,沉淀细胞,尽量去除胰酶细胞消化液后,
      加入含血清的完全培养液重新悬浮细胞,即可用于后续实验。
2. 组织的消化:
   不同的组织需要消化的时间相差很大,通常以消化后可以充分打散组织为宜。

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