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Actin抗体 |
产品编号: AA128
产品包装: >40次
产品价格: 398.00元 |
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产品简介
产品简介:
产品编号 |
产品名称 |
产品包装 |
产品价格 |
AA128 |
Actin抗体 |
>40次 |
398.00元 |
来源 |
用途 |
交叉反应性 |
抗体类型 |
β-actin分子量 |
Mouse |
WB, IF, IHC |
Mam, C, CP, L, F |
IgG2a |
~42kD |
WB, Western blot; IF, Immunofluorescence; IHC, Immunohistochemistry.
Mam, mammalian, including human, mouse, rat, bovine, sheep, pig, rabbit, cat, dog,
guinea pig; C, chicken; CP, carp; L, leech; F, fruit fly.
本Actin抗体(Actin antibody)为进口分装,用经过适当修饰的人工合成β-actin N-terminal一段
多肽作为抗原制备而成的抗β-actin小鼠单克隆抗体。克隆号为AC-74。本Actin抗体在Western blot或免疫染色时仅识别β-actin。注意:本抗体不能用于检测成年动物心肌或骨骼肌的β-actin。
Actin即肌动蛋白,是细胞的一种重要骨架蛋白。同时Actin在细胞分泌、吞噬、locomotion、
cytoplasmic streaming和cytokinesis等过程中起重要作用。Actin在不同物种之间高度保守,以至于很难获得较好的针对actin的抗血清。Actin大致可分为六种,其中四种是不同肌肉组织特异性的,包括α-skeletal muscle actin,α-cardiac muscle actin,α-smooth muscle actin,和γ-smooth muscle actin; 其余两种广泛分布于各种组织中,包括β-actin(β-non-muscle)和γ-non-muscle actin。不同的actin之间同源性大于90%,但在N-terminal同源性仅50%-60%,因此N-terminal常被用作actin的抗原。
β-actin的蛋白水平通常不会发生改变,因此被广泛用于Western时上样量是否一致的参照。也常被
用于免疫染色观察细胞的微丝结构。在用作Western的参照时,Actin抗体和Tubulin抗体的主要不同之处在于两者所识别蛋白的分子量不同,这样可以选择合适的参照在同一块胶同一张膜上实现同时检测目标蛋白和参照蛋白。
配套提供了Western一抗稀释液,可以用于Western检测时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB |
IF |
IHC |
1:1000 |
1:400 |
1:400 |
本抗体如果用于常规的Western检测,至少可以检测40次。
包装清单:
产品编号 |
产品名称 |
包装 |
AA128-1 |
Actin抗体 |
40μl |
AA128-2 |
Western一抗稀释液 |
40ml |
- |
说明书 |
1份 |
保存条件:
Actin抗体-20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
在Western实验后,请注意回收稀释的抗体。回收的抗体在进行Western实验时至少可以重复使用10
次。稀释后的抗体,包括已经使用过的稀释抗体,4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混
浊现象,可以10000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明
产品图片
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