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GFP抗体 |
产品编号: AG279
产品包装: >40次
产品价格: 408.00元 |
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产品简介
产品简介:
产品编号 |
产品名称 |
产品包装 |
产品价格 |
AG279 |
GFP抗体 |
>40次 |
408.00元 |
来源 |
用途 |
抗体识别位点 |
GFP分子量 |
Rabbit |
WB, IP, IF |
Full length GFP |
~27kD |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence.
本GFP抗体(GFP antibody)为进口分装,用全长的来源于水母的GFP(green fluorescent protein)免疫rabbit,然后用protein A和抗原多肽亲和柱经过两步纯化得到的高纯度抗体。
本GFP抗体可以识别GFP以及GFP的一些突变体例如EGFP(enhanced green fluorescent protein)。
GFP或其突变体EGFP等被广泛用于基因表达效率的检测,以及和目的蛋白融合表达用于检测目的蛋白的表达和分布。本GFP抗体不仅可以检测GFP或其适当的突变体,也可以检测和GFP或其适当的突变体融合表达的蛋白。
配套提供了Western一抗稀释液,可以用于Western检测时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据GFP表达水平的高低作适当调整):
WB |
IP |
IF |
1:200-1000 |
1:100 |
1:200 |
本抗体用于常规的Western检测,如果稀释比例为1:1000,则至少可以检测40次。
包装清单:
产品编号 |
产品名称 |
包装 |
AG279-1 |
GFP抗体 |
40μl |
AG279-2 |
Western一抗稀释液 |
40ml |
- |
说明书 |
1份 |
保存条件:
GFP抗体-20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
对于本抗体,Western检测时一抗要4℃缓慢摇动过夜,如果仅短时间与一抗孵育检测效果较差。
在Western实验后,请注意回收稀释的抗体。回收的抗体在进行Western实验时至少可以重复使用10
次。稀释后的抗体,包括已经使用过的稀释抗体,4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混
浊现象,可以10000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明
产品图片
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