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BCA蛋白浓度测定试剂盒 |
产品编号: P0011
产品包装: 5000次
产品价格: 1398.00元 |
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产品简介
产品简介:
BCA蛋白浓度测定试剂盒(BCA Protein Assay Kit)是根据目前世界上最常用的两种蛋白浓度检测方
法之一BCA法研制而成,实现了蛋白浓度测定的简单、高稳定性、高灵敏度和高兼容性。
灵敏度高,检测浓度下限达到25μg/ml,最小检测蛋白量达到0.5μg,待测样品体积为1-20μl。
在50-2000μg/ml浓度范围内有较好的线性关系。
BCA法测定蛋白浓度不受绝大部分样品中的化学物质的影响,可以兼容样品中高达5%的SDS,5%
的Triton X-100,5%的Tween 20、60、80。但本试剂盒受螯合剂和略高浓度的还原剂的影响,需确保EDTA低于10mM,无EGTA,二硫苏糖醇(DTT)低于1mM,β-巯基乙醇(β-Mercaptoethanol)低于0.01%。不适用BCA法时建议试用碧云天生产的Bradford蛋白浓度测定试剂盒(P0006)。
BCA蛋白浓度测定试剂盒对样品中各种物质的详细的兼容性,请参见碧云天如下网页:
http://www.beyotime.com/Compatibility Chart For BCA Kit.pdf
每个试剂盒可以检测5000个样品。
包装清单:
产品编号 |
产品名称 |
包装 |
P0011-1 |
BCA试剂 A |
500ml×2 |
P0011-2 |
BCA试剂 B |
30ml |
P0011-3 |
蛋白标准(BSA) |
30mg×2 |
P0011-4 |
蛋白标准配制液 |
5ml |
— |
说明书 |
1份 |
保存条件:
室温保存。蛋白标准配制成溶液后-20℃冻存。
注意事项:
需酶标仪一台,测定波长为540-595nm之间,562nm最佳。需96孔板。如果没有酶标仪,也可以使用
普通的分光光度计测定,但测定时,需根据比色皿的最小检测体积,适当加大BCA工作液的用量使不小于最小检测体积,样品和标准品的用量可相应按比例放大也可不变。使用分光光度计测定蛋白浓度时,每个试剂盒可以测定的样品数量可能会显著减少。
如发现样品稀释液或裂解液本身就有较高背景,请试用碧云天生产的Bradford蛋白浓度测定试剂盒
(P0006)。
为了加快BCA法测定蛋白浓度的速度可以适当用微波炉加热,但是切勿过热。
EDTA浓度必须小于10mM,不兼容EGTA。不适用BCA法时,请试用碧云天生产的Bradford蛋白浓度测定试剂盒(P0006)。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明
使用说明:
1. 蛋白标准品的准备
a. 取1.2ml蛋白标准配制液加入到一管蛋白标准(30mg BSA)中,充分溶解后配制成25mg/ml的蛋白标
准溶液。配制后可立即使用,也可以-20℃长期保存。
b. 取适量25mg/ml蛋白标准,稀释至终浓度为0.5mg/ml。例如取20μl 25mg/ml蛋白标准,加入980μl
稀释液即可配制成0.5mg/ml蛋白标准。蛋白样品在什么溶液中,标准品也宜用什么溶液稀释。但
是为了简便起见,也可以用0.9%NaCl或PBS稀释标准品。稀释后的0.5mg/ml蛋白标准可以-20℃长
期保存。
2. BCA工作液配制
根据样品数量,按50体积BCA试剂A加1体积BCA试剂B(50:1)配制适量BCA工作液,充分混匀。例如5ml
BCA试剂A加100μl BCA试剂B,混匀,配制成5.1ml BCA工作液。BCA工作液室温24小时内稳定。
3. 蛋白浓度测定
a. 将标准品按0、1、2、4、8、12、16、20μl加到96孔板的标准品孔中,加标准品稀释液补足到
20μl,相当于标准品浓度分别为0、0.025、0.05、0.1、0.2、0.3、0.4、0.5mg/ml。
b. 加适当体积样品到96孔板的样品孔中。如果样品不足20μl,需加标准品稀释液补足到20μl。请注意
记录样品体积。
c. 各孔加入200μl BCA工作液,37℃放置20-30分钟。
注: 也可以室温放置2小时,或60℃放置30分钟。BCA法测定蛋白浓度时,颜色会随着时间的延长
不断加深。并且显色反应会因温度升高而加快。如果浓度较低,适合在较高温度孵育,或适当延
长孵育时间。
d. 用酶标仪测定A562,或540-595nm之间的其他波长的吸光度。
e. 根据标准曲线和使用的样品体积计算出样品的蛋白浓度。
常见问题:
1. 测定标准曲线时发现随着标准品浓度的增加吸光度或颜色没有明显变化。
可能的原因是样品中含有严重干扰BCA法测定蛋白浓度的物质,详细的BCA法的兼容性列表请参考碧云
天如下网页:http://www.beyotime.com/Compatibility Chart For BCA Kit.pdf
2. 是否每次测定时都需要做标准曲线?
建议每次测定时都做标准曲线。因为BCA法测定时颜色会随着时间的延长不断加深,并且显色反应的
速度和温度有关,所以除非精确控制显色反应的时间和温度,否则如需精确测定宜每次都做标准曲
线。
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Responses of vascular smooth muscle cells to estrogen are dependent on balance between
ERK and p38 MAPK pathway activities.
Int J Cardiol. 2009 May 29;134(3):356-65.
76.Chen MF, Li YJ, Yang TL, Lou B, Xie XM.
Losartan inhibits monocytic adhesion induced by ADMA via downregulation of chemokine
receptors in monocytes.
Eur J Clin Pharmacol. 2009 May;65(5):457-64. Epub 2009 Jan 22.
77.Zhang DL, Chen YQ, Jiang X, Ji TT, Mei B.
Oxidative damage increased in presenilin1/presenilin2 conditional double knockout mice.
Neurosci Bull. 2009 Jun;25(3):131-7.
78.Wang XD, Meng MX, Gao LB, Liu T, Xu Q, Zeng S.
Permeation of astilbin and taxifolin in Caco-2 cell and their effects on the P-gp.
Int J Pharm. 2009 Aug 13;378(1-2):1-8.
79.Chen B, Gao XQ, Yang CX, Tan SK, Sun ZL, Yan NH, Pang YG, Yuan M, Chen GJ, Xu GT, Zhang
K, Yuan QL.
Neuroprotective effect of grafting GDNF gene-modified neural stem cells on cerebral
ischemia in rats.
Brain Res. 2009 Aug 11;1284:1-11.
80.Ding D, Huang P, Sun H, Xue Q, Pan Y, Chen S.
Identification of protein components and quantitative immunoassay for SEC2 in
staphylococcin injection.
J Pharm Biomed Anal. 2009 Aug 15;50(1):79-85.
81.Liu X, Shi S, Ye J, Liu L, Sun M, Wang C.
Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-kappaB/COX-2 activation
and apoptosis in HaCaT cells.
J Photochem Photobiol B. 2009 Aug 3;96(2):109-16.
82.Zhang Z, Wang Q, Li P, Zhou Y, Li S, Yi W, Chen A, Kong P, Hu C.
Overexpression of the Interferon regulatory factor 4-binding protein in human colorectal
cancer and its clinical significance.
Cancer Epidemiol. 2009 Aug;33(2):130-6. Epub 2009 Jul 10.
83.Ma K, Hu M, Qi Y, Qiu L, Jin Y, Yu J, Li B.
Structure-transfection activity relationships with glucocorticoid-polyethyl-enimine
conjugate nuclear gene delivery systems.
Biomaterials. 2009 Aug;30(22):3780-9. Epub 2009 Apr 17.
84.Liao Z, Huang C, Zhou F, Xiong J, Bao J, Zhang H, Sun W, Xie C, Zhou Y.
Radiation enhances suicide gene therapy in radioresistant laryngeal squamous cell
carcinoma via activation of a tumor-specific promoter.
Cancer Lett. 2009 Sep 28;283(1):20-8.
85.Feng B, Huang CG, Chen RH, Guo YW, Jiao BH.
2'-Epi-2'-O-acetylthevetin B induces apoptosis partly via Ca(2+)-mediated mitochondrial
pathway in human hepatocellular carcinoma HepG2 cells.
Cell Biol Int. 2009 Sep;33(9):918-25.
86.Ye Y, Hu SJ, Li L.
Inhibition of farnesylpyrophosphate synthase prevents angiotensin II-induced
hypertrophic responses in rat neonatal cardiomyocytes: involvement of the RhoA/Rho
kinase pathway.
FEBS Lett. 2009 Sep 17;583(18):2997-3003.
87.Shi GS, Ren LF, Wang LZ, Lin HS, Wang SB, Tong YQ.
H2O2/HCl and heat-treated Ti-6Al-4V stimulates pre-osteoblast proliferation and
differentiation.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Sep;108(3):368-75.
88.Zhu J, Zhang L, Jin X, Han X, Sun C, Yan J.
beta-Ionone-induced apoptosis in human osteosarcoma (U2os) cells occurs via a
p53-dependent signaling pathway.
Mol Biol Rep. 2009 Sep 16.
89.Chen Y, Wang J, Yao Y, Yuan W, Kong M, Lin Y, Geng D, Nie R.
CRP regulates the expression and activity of tissue factor as well as tissue factor
pathway inhibitor via NF-kappaB and ERK 1/2 MAPK pathway.
FEBS Lett. 2009 Sep 3;583(17):2811-8. Epub 2009 Jul 23.
90.Chen WL, Qian Y, Meng WF, Pang JY, Lin YC, Guan YY, Chen SP, Liu J, Pei Z, Wang GL.
A novel marine compound xyloketal B protects against oxidized LDL-induced cell injury in
vitro.
Biochem Pharmacol. 2009 Oct 15;78(8):941-50.
91.Sun H, Li QW, Lv XY, Ai JZ, Yang QT, Duan JJ, Bian GH, Xiao Y, Wang YD, Zhang Z, Liu
YH, Tan RZ, Yang Y, Wei YQ, Zhou Q.
MicroRNA-17 post-transcriptionally regulates polycystic kidney disease-2 gene and
promotes cell proliferation.
Mol Biol Rep. 2009 Oct 10.
92.Wang S, Lv X, Zhang K, Lin T, Liu X, Yuan J, Dai Y, Li N.
Knockdown of the prion gene expression by RNA interference in bovine fibroblast cells.
Mol Biol Rep. 2009 Oct 11.
93.Ma K, Hu MX, Qi Y, Zou JH, Qiu LY, Jin Y, Ying XY, Sun HY.
PAMAM-triamcinolone acetonide conjugate as a nucleus-targeting gene carrier for enhanced
transfer activity.
Biomaterials. 2009 Oct;30(30):6109-18. Epub 2009 Aug 4.
94.Hua W, Jiang J, Rong X, Wu R, Qiu H, Zhang Y, Chen Q.
The dual role of the cystathionine gamma-lyase/hydrogen sulfide pathway in CVB3-induced
myocarditis in mice.
Biochem Biophys Res Commun. 2009 Oct 23;388(3):595-600. Epub 2009 Aug 18.
95.Jin D, Ojcius DM, Sun D, Dong H, Luo Y, Mao Y, Yan J.
Leptospira interrogans induces apoptosis in macrophages via caspase-8- and caspase-3-
dependent pathways.
Infect Immun. 2009 Feb;77(2):799-809. Epub 2008 Nov 24.
96.Lin Y, Chen Y, Yang X, Xu D, Liang S.
Proteome analysis of a single zebrafish embryo using three different digestion
strategies coupled with liquid chromatography-tandem mass spectrometry.
Anal Biochem. 2009 Nov 15;394(2):177-85.
97.Xu Y, Ge R, Du J, Xin H, Yi T, Sheng J, Wang Y, Ling C.
Corosolic acid induces apoptosis through mitochondrial pathway and caspase activation in
human cervix adenocarcinoma HeLa cells.
Cancer Lett. 2009 Nov 1;284(2):229-37.
98.Li H, Zhang L, Huang Q.
Differential expression of mitogen-activated protein kinase signaling pathway in the
hippocampus of rats exposed to chronic unpredictable stress.
Behav Brain Res. 2009 Dec 14;205(1):32-7. Epub 2009 Jul 1.
99.Yi Q, Wen X, Li L, He B, Nie Y, Yao Wu Y, Zhang Z, Gu Z.
The chiral effects on the responses of osteoblastic cells to the polymeric substrates.
European Polymer Journal 45 (2009) 1970–1978.
100.ZHANG ZY, LIU XJ, LIU T, YAN L, WANG YJ, WANG CB.
Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via
iNOS/NO and HSP90.
Chinese Journal of Oceanology and Limnology. Vol. 27 No. 3, P. 594-599, 2009.
101.Deng X, Cheng J, Zhang Y, Li N, Chen L.
Effects of caloric restriction on SIRT1 expression and apoptosis of islet beta cells in
type 2 diabetic rats.
Acta Diabetol. 2009.
102.WU T, NAN KH, CHEN JD, JIN D, JIANG S, ZHAO PR, XU JC, DU H, ZHANG XQ, LI JW, PEI GX.
A new bone repair scaffold combined with chitosan/hydroxyapatite and sustained releasing
icariin.
Chinese Science Bulletin. 2009; vol. 54 no. 17.
103.Fan L, Lin C, Zhuo S, Chen L, Liu N, Luo Y, Fang J, Huang Z, Lin Y, Chen J.
Transplantation with survivin-engineered mesenchymal stem cells results in better
prognosis in a rat model of myocardial infarction.
Eur J Heart Fail. 2009;11(11):1023-30.
104.Liu XJ, Wang WC, Wang HJ, Zhang LL, Liu LQ, Wang YJ, Wang CB.
A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the
Apaf-1/caspase-9 and Smac/XIAP signaling pathway.
Chinese Journal of Oceanology and Limnology.Vol. 27 No. 3, P. 587-593, 2009
105.Gong HS, Meng XC, Wang H.
Plantaricin MG active against Gram-negative bacteria produced by Lactobacillus plantarum
KLDS1.0391 isolated from ‘‘Jiaoke”, a traditional fermented cream from China.
Food Control 21 (2010) 89–96.
106.Zhou CL, Yu XJ, Chen LM, Jiang H, Li CY.
Corticotropin-releasing hormone attenuates vascular endothelial growth factor release
from human HaCaT keratinocytes.
Regul Pept. 2010 Feb 25;160(1-3):115-20.
107.Chen S, Chen Y, Hu C, Jing H, Cao Y, Liu X.
Association of clinicopathological features with UbcH10 expression in colorectal cancer.
J Cancer Res Clin Oncol. 2010 Mar;136(3):419-26.
108.Yuan D, Pan Y, Zhang J, Shao C.
Role of nuclear factor-kappaB and P53 in radioadaptive response in Chang live cells.
Mutat Res. 2010 Jun 1;688(1-2):66-71. Epub 2010 Mar 20.
109.Li F, Gong QH, Wu Q, Lu YF, Shi JS.
Icariin isolated from Epimedium brevicornum Maxim attenuates learning and memory
deficits induced by d-galactose in rats.
Pharmacol Biochem Behav. 2010;96(3):301-5. Epub 2010 Jun 1.
110.Li H, Fong C, Chen Y, Cai G, Yang M.
beta2- and beta3-, but not beta1-adrenergic receptors are involved in osteogenesis of
mouse mesenchymal stem cells via cAMP/PKA signaling.
Arch Biochem Biophys. 2010 15;496(2):77-83. Epub 2010 Feb 4.
111.Wu GJ, Zhou LZ, Wang KW, Chen F, Sun Y, Duan YR, Zhu YJ, Gu HC.
Hydroxylapatite nanorods: An efficient and promising carrier for gene transfection.
J Colloid Interface Sci. 2010.
112.Han J, Zhou YP, Jiang YZ, He YT, Mei F.
Postnatal development of interstitial cells of Cajal in mouse colon in response to Kit
signal blockade with Imatinib (Glivec).
Acta Histochem. 2010;112(3):215-21. Epub 2010 Mar 2.
113.Feng B, Guo YW, Huang CG, Li L, Chen RH, Jiao BH.
2'-epi-2'-O-Acetylthevetin B extracted from seeds of Cerbera manghas L. induces cell
cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.
Chem Biol Interact. 2010;183(1):142-53. Epub .
114.Qin XL, Bi HC, Wang XD, Li JL, Wang Y, Xue XP, Chen X, Wang CX, Xu le J, Wang YT,
Huang M.
Mechanistic understanding of the different effects of Wuzhi Tablet (Schisandra
sphenanthera extract) on the absorption and first-pass intestinal and hepatic metabolism
of Tacrolimus (FK506).
Int J Pharm. 2010;389(1-2):114-21. Epub 2010 Jan 25.
115.Lin Y, Xu J, Cao L, Han Y, Gao J, Xie N, Zhao X, Jiang H, Chi Z.
Mitochondrial base excision repair pathway failed to respond to status epilepticus
induced by pilocarpine.
Neurosci Lett. 2010;474(1):22-5. Epub 2010 Mar 3.
116.Li Q, Wu D, Zhang L, Zhang Y.
Effects of galantamine on β-amyloid release and beta-site cleaving enzyme 1 expression
in differentiated human neuroblastoma SH-SY5Y cells.
Exp Gerontol. 2010;45(11):842-7. Epub 2010 Jul 3.
117.Li GG, Liang XF, Xie Q, Li G, Yu Y, Lai K.
Gene structure, recombinant expression and functional characterization of grass carp
leptin.
Gen Comp Endocrinol. 2010;166(1):117-27. Epub 2009 Oct 24.
118.Zhao L, Wang X, Chang Q, Xu J, Huang Y, Guo Q, Zhang S, Wang W, Chen X, Wang J.
Neferine, a bisbenzylisoquinline alkaloid attenuates bleomycin-induced pulmonary
fibrosis.
Eur J Pharmacol. 2010;627(1-3):304-12. Epub 2009 Nov 10.
119.Zhao L, Yang R, Cheng L, Wang M, Jiang Y, Wang S.
LPS-Induced Epithelial-Mesenchymal Transition of Intrahepatic Biliary Epithelial Cells.
J Surg Res. 2010.
120.He F, Zhang F, Yang G, Wang X, Zhao S.
Enhanced initial proliferation and differentiation of MC3T3-E1 cells on HF/HNO3 solution
treated nanostructural titanium surface.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;110(4):e13-22. Epub 2010
Jul 15.
121.Yan J, Sun XB, Wang HQ, Zhao H, Zhao XY, Xu YX, Guo JC, Zhu CQ.
Chronic restraint stress alters the expression and distribution of phosphorylated tau
and MAP2 in cortex and hippocampus of rat brain.
Brain Res. 2010;1347:132-41. Epub 2010 Jun 1.
122.Song XZ, Xu JQ, Wang T, Liu FH.
Traditional Chinese medicine decoction enhances growth performance and intestinal
glucose absorption in heat stressed pigs by up-regulating the expressions of SGLT1 and
GLUT2 mRNA.
Livestock Science 128 (2010) 75–81.
123.Yuan H, Zhang W, Du YZ, Hu FQ.
Ternary nanoparticles of anionic lipid nanoparticles/protamine/DNA for gene delivery.
Int J Pharm. 2010;392(1-2):224-31. Epub 2010 Mar 15.
124.Yu Q, Zhao Z, Sun J, Guo W, Fu J, Burnstock G, He C, Xiang Z.
Expression of P2X6 receptors in the enteric nervous system of the rat gastrointestinal
tract.
Histochem Cell Biol. 2010;133(2):177-88. Epub 2009 Nov 28.
125.Hu H, Zhang Y, Zou M, Yang S, Liang XQ.
Expression of TRF1, TRF2, TIN2, TERT, KU70, and BRCA1 proteins is associated with
telomere shortening and may contribute to multistage carcinogenesis of gastric cancer.
J Cancer Res Clin Oncol. 2010;136(9):1407-14. Epub 2010 Feb 2.
126.Ding XL, Wang YH, Ning LP, Zhang Y, Ge HY, Jiang H, Wang R, Yue SW.
Involvement of TRPV4-NO-cGMP-PKG pathways in the development of thermal hyperalgesia
following chronic compression of the dorsal root ganglion in rats.
Behav Brain Res. 2010;208(1):194-201. Epub 2009 Dec 3.
127.Chen Z, Liu S, Sumida T, Sun S, Wei Y, Liu M, Dong Z, Zhang F, Hamakawa H, Wei F.
Silencing Id-1 with RNA Interference Inhibits Adenoid Cystic Carcinoma in Mice.
J Surg Res. 2010. [Epub ahead of print].
128.Zhang JC, Liu CL, Li YP, Sun J, Wang P, Di KQ, Chen H, Zhao YY .
Effect of yttrium ion on the proliferation, differentiation and mineralization function
of primary mouse osteoblasts in vitro.
Journal Of Rare Earths. Vol. 28, No. 3, Jun. 2010, p. 466.
129.Huang LH, Hu JQ, Tao WQ, Li YH, Li GM, Xie PY, Liu XS, Jiang J.
Gossypol inhibits phosphorylation of Bcl-2 in human leukemia HL-60 cells.
Eur J Pharmacol. 2010;645(1-3):9-13. Epub 2010 Jul 13.
130.Sun J, Liu X, Li Q.
Molecular cloning, expression and antioxidant activity of a peroxiredoxin 2 homologue
from Lampetra japonica.
Fish Shellfish Immunol. 2010;28(5-6):795-801. Epub 2010 Feb 6.
131.Wang S, Liu S, Liu H, Wang J, Zhou S, Jiang RJ, Bendena WG, Li S.
20-hydroxyecdysone reduces insect food consumption resulting in fat body lipolysis
during molting and pupation.
J Mol Cell Biol. 2010;2(3):128-38. Epub 2010 Apr 29.
132.Cao BY, Yang YP, Luo WF, Mao CJ, Han R, Sun X, Cheng J, Liu CF.
Paeoniflorin, a potent natural compound, protects PC12 cells from MPP+ and acidic damage
via autophagic pathway.
J Ethnopharmacol. 2010;131(1):122-9. Epub 2010 Jun 15.
133.Liu J, Wang A, Li L, Huang Y, Xue P, Hao A.
Oxidative stress mediates hippocampal neuron death in rats after lithium-pilocarpine-
induced status epilepticus.
Seizure. 2010;19(3):165-72. Epub 2010 Feb 10.
134.Li C, Xing G, Dong M, Zhou L, Li J, Wang G, Zou D, Wang R, Liu J, Niu Y.
Beta-asarone protection against beta-amyloid-induced neurotoxicity in PC12 cells via JNK
signaling and modulation of Bcl-2 family proteins.
Eur J Pharmacol. 2010;635(1-3):96-102. Epub 2010 Mar 20.
135.Wu W, Yan C, Gan T, Chen Z, Lu X, Duerksen-Hughes PJ, Zhu X, Yang J.
Nuclear proteome analysis of cisplatin-treated HeLa cells.
Mutat Res. 2010;691(1-2):1-8. Epub 2010 Jun 9.
136.Baohua Y, Li L.
Effects of SIRT6 silencing on collagen metabolism in human dermal fibroblasts.
Cell Biol Int. 2012 Jan;36(1):105-8.
137.Han X, Lu M, Wang S, Lv D, Liu H
Targeting IKK/NF-κB pathway reduces infiltration of inflammatory cells and apoptosis
after spinalcord injury in rats.
Neurosci Lett. 2012 Mar 5;511(1):28-32.
138.Xiao O, Xie ZL, Lin BW, Yin XF, Pi RB, Zhou SY
Minocycline inhibits alkali burn-induced corneal neovascularization in mice.
PLoS One. 2012;7(7):e41858.
139.Liu Z, Zhang J, Huang X, Huang L, Li S, Wang Z
Magnesium sulfate inhibits the secretion of high mobility group box 1 from
lipopolysaccharide-activated RAW264.7 macrophages in vitro.
J Surg Res. 2012 Mar 28.
140.Yang XH, Wang YH, Wang JJ, Liu YC, Deng W, Qin C, Gao JL, Zhang LY
Role of angiotensin-converting enzyme (ACE and ACE2) imbalance on tourniquet-induced
remotekidney injury in a mouse hindlimb ischemia-reperfusion model.
Peptides. 2012 Jul;36(1):60-70.
141.Liu H, Chen X, Gao W, Jiang G.
The expression of heparanase and microRNA-1258 in human non-small cell lung cancer.
Tumour Biol. 2012 Apr 10.
142.Li TT, Zhang YS, He L, Liu B, Shi RZ, Zhang GG, Peng J
Inhibition of vascular peroxidase alleviates cardiac dysfunction and apoptosis induced
by ischemia-reperfusion.
Can J Physiol Pharmacol. 2012 Jul;90(7):851-62.
143.Zhang L, Ding W, Sun H, Zhou Q, Huang J, Li X, Xie Y, Chen J
Salidroside protects PC12 cells from MPP(+)-induced apoptosis via activation of the
PI3K/Akt pathway.
Food Chem Toxicol. 2012 Aug;50(8):2591-7.
144.Yu SJ, Liu HC, Ling-Ling E, Wang DS, Zhu GX
Proliferation and differentiation of osteoblasts from the mandible of osteoporotic rats.
Exp Biol Med (Maywood). 2012 Apr;237(4):395-406.
145.Jiang L, Lin J, Han H, Dong H, Zhao Q, Zhu S, Huang B.
Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1).
PLoS One. 2012;7(7):e41115.
146.Chen M, Hu M, Wang D, Wang G, Zhu X, Yan D, Sun J.
Multifunctional Hyperbranched Glycoconjugated Polymers Based on Natural Aminoglycosides.
Bioconjug Chem. 2012 May 25.
147.Sun D, Wang Y, Liu C, Zhou X, Li X, Xiao A
Effects of nitric oxide on renal interstitial fibrosis in rats with unilateral ureteral
obstruction.
Life Sci. 2012 Jun 14;90(23-24):900-9.
148.Zhang YS, He L, Liu B, Li NS, Luo XJ, Hu CP, Ma QL, Zhang GG, Li YJ, Peng J
A novel pathway of NADPH oxidase/vascular peroxidase 1 in mediating oxidative injury
followingischemia-reperfusion.
Basic Res Cardiol. 2012;107(3):266. Epub 2012 Apr 3.
149.Zhu C, Wang Q, Xie J, Shi J, Zhou X, Li D, Xiong F, Zhang L
Expression and Significance of RKIP and E-cadherin in Lung Squamous Cell Carcinoma.
Pathol Oncol Res. 2012 Jun 13.
150.Zhao P, Han T, Guo JJ, Zhu SL, Wang J, Ao F, Jing MZ, She YL, Wu ZH, Ye LB.
HCV NS4B induces apoptosis through the mitochondrial death pathway.
Virus Res. 2012 Apr 19.
151.Zhang Y, Xu Y, Zhu Q, Zhao F, Luo J, Zhang X, Wang X
Upregulation of dysbindin in temporal lobe epileptic foci of human and experimental
animals.
Synapse. 2012 Jul;66(7):622-9. doi: 10.1002/syn.21548. Epub 2012 Mar 30.
152.Zhang X, Xu Y, Wang J, Zhou Q, Pu S, Jiang W, Du D.
The effect of intrathecal administration of glial activation inhibitors on dorsal horn
BDNFoverexpression and hind paw mechanical allodynia in spinal nerve ligated rats.
J Neural Transm. 2012 Mar;119(3):329-36.
153.Song Z, Liu L, Yue Y, Jiao H, Lin H, Sheikhahmadi A, Everaert N, Decuypere E, Buyse J.
Fasting alters protein expression of AMP-activated protein kinase in the hypothalamus of
broilerchicks (Gallus gallus domesticus).
Gen Comp Endocrinol. 2012 Jul 5.
154.Lu N, Liu Q, Li R, Xie L, Shen J, Guan W, Qian X, Yu L, Ding Y, Jiang X, Liu B.
Superior antimetastatic effect of pemetrexed-loaded gelatinase-responsive nanoparticles
in amouse metastasis model.
Anticancer Drugs. 2012 Jul 11. [Epub ahead of print]
155.Xu MF, Xiong YY, Liu JK, Qian JJ, Zhu L, Gao J
Asiatic acid, a pentacyclic triterpene in Centella asiatica, attenuates
glutamate-induced cognitivedeficits in mice and apoptosis in SH-SY5Y cells.
Acta Pharmacol Sin. 2012 May;33(5):578-87.
156.Zhao XY, Zhu YJ, Chen F, Wu J.
Calcium phosphate nanocarriers dual-loaded with bovine serum albumin and Ibuprofen:
facilesynthesis, sequential drug loading and sustained drug release.
Chem Asian J. 2012 Jun;7(7):1610-5.
157.Hu B, Zhou H, Gao H, Liu Y, Yan T, Zou L, Chen L.
IFN-γ Inhibits Osteopontin Expression in Human Decidual Stromal Cells and can be
Attenuated by 1α,25-Dihydroxyvitamin D3.
Am J Reprod Immunol. 2012 Jul 11.
158.Yang L, Cheng F, Liu T, Lu JR, Song K, Jiang L, Wu S, Guo W
Comparison of mesenchymal stem cells released from poly(N-isopropylacrylamide)
copolymerfilm and by trypsinization.
Biomed Mater. 2012 Jun;7(3):035003.
159.Zhang Q, He QF, Zhang TH, Yu XL, Liu Q, Deng FL
Improvement in the delivery system of bone morphogenetic protein-2: a new approach to
promotebone formation.
Biomed Mater. 2012 Aug;7(4):045002.
160.Sun X, Cheng F, Meng B, Yang B, Song W, Yuan H
Pregnenolone sulfate decreases intraocular pressure and changes expression of sigma
receptorin a model of chronic ocular hypertension.
Mol Biol Rep. 2012 Jun;39(6):6607-14.
161.Wang T, Fu F, Han B, Zhang L, Zhang X.
Danshensu ameliorates the cognitive decline in streptozotocin-induced diabetic mice
byattenuating advanced glycation end product-mediated neuroinflammation.
J Neuroimmunol. 2012 Apr;245(1-2):79-86.
162.Ren M, Qin D, Li K, Qu J, Wang L, Wang Z, Huang A, Tang H
Correlation between hepatitis B virus protein and microRNA processor Drosha in cells
expressingHBV.
Antiviral Res. 2012 Jun;94(3):225-31.
163.Zhang Y, Zhou L, Zhang X, Bai J, Shi M, Zhao G.
Ginsenoside-Rd attenuates TRPM7 and ASIC1a but promotes ASIC2a expression in rats after
focalcerebral ischemia.
Neurol Sci. 2012 Jan 10.
164.Bi WY, Fu BD, Shen HQ, Wei Q, Zhang C, Song Z, Qin QQ, Li HP, Lv S, Wu SC, Yi PF,
Wei XB
Sulfated Derivative of 20(S)-Ginsenoside Rh2 Inhibits Inflammatory Cytokines Through
MAPKs andNF-kappa B Pathways in LPS-Induced RAW264.7 Macrophages.
Inflammation. 2012 May 22.
165.Yu H, Li Q, Zhou X, Kolosov VP, Perelman JM
Transient receptor potential vanilloid 1 receptors mediate acid-induced mucin secretion
via Ca2+influx in human airway epithelial cells.
J Biochem Mol Toxicol. 2012 May;26(5):179-86.
166.Tian J, Yan J, Wang W, Zhong N, Tian L, Sun J, Min Z, Ma J, Lu S.
T-2 toxin enhances catabolic activity of hypertrophic chondrocytes through
ROS-NF-κB-HIF-2αpathway.
Toxicol In Vitro. 2012 Jul 16.
167.Huang Q, Deng J, Xie Z, Wang F, Chen S, Lei B, Liao P, Huang N, Wang Z, Wang Z,
Cheng Y.
Effective gene transfer into central nervous system following
ultrasound-microbubbles-inducedopening of the blood-brain barrier.
Ultrasound Med Biol. 2012 Jul;38(7):1234-43.
168.Wu J, Lai G, Wan F, Xiao Z, Zeng L, Wang X, Ye F, Lei T.
Knockdown of checkpoint kinase 1 is associated with the increased radiosensitivity
ofglioblastoma stem-like cells.
Tohoku J Exp Med. 2012;226(4):267-74.
169.Chen X, Yang X, Liu T, Guan M, Feng X, Dong W, Chu X, Liu J, Tian X, Ci X, Li H,
Wei J, Deng Y, Deng X, Chi G, Sun Z.
Kaempferol regulates MAPKs and NF-κB signaling pathways to attenuate LPS-induced acute
lunginjury in mice.
Int Immunopharmacol. 2012 Jul 23.
170.Fauzee NJ, Wang YL, Dong Z, Li QG, Wang T, Mandarry MT, Xu L, Pan J
Novel hydrophilic docetaxel (CQMU-0519) analogue inhibits proliferation and induces
apoptosis inhuman A549 lung, SKVO3 ovarian and MCF7 breast carcinoma cell lines.
Cell Prolif. 2012 Aug;45(4):352-64.
171.Li XY, Jing CQ, Zang XY, Yang S, Wang JJ.
Toxic cytological alteration and mitochondrial dysfunction in PC12 cells induced by
1-octyl-3-methylimidazolium chloride.
Toxicol In Vitro. 2012 Jul 23.
172.Zhou YM, Zhang XF, Cao L, Li B, Sui CJ, Li YM, Yin ZF
MCM7 expression predicts post-operative prognosis for hepatocellular carcinoma.
Liver Int. 2012 Jul 12.
173.Lin N, Chen H, Zhang H, Wan X, Su Q.
Mitochondrial reactive oxygen species (ROS) inhibition ameliorates palmitate-induced
INS-1 betacell death.
Endocrine. 2012 Aug;42(1):107-17.
174.Liu P, Shi Y, Yang Y, Cao Y, Shi Y, Li H, Liu J, Lin J, Jin Y.
Schistosoma Japonicum UDP-Glucose 4-Epimerase Protein Is Located on the Tegument
andInduces Moderate Protection against Challenge Infection.
PLoS One. 2012;7(7):e42050.
175.Du HM, Zheng XH, Wang LY, Tang W, Liu L, Jing W, Lin YF, Tian WD, Long J
The Osteogenic Response of Undifferentiated Human Adipose-Derived Stem Cells
underMechanical Stimulation.
Cells Tissues Organs. 2012 May 15.
176.Li XJ, Liu DP, Chen HL, Pan XH, Kong QY, Pang QF
Lactoferrin protects against lipopolysaccharide-induced acute lung injury in mice.
Int Immunopharmacol. 2012 Feb;12(2):460-4.
177.Zuo K, Li D, Pulli B, Yu F, Cai H, Yuan X, Zhang X, Lv Z.
Short-hairpin RNA-mediated Heat shock protein 90 gene silencing inhibits human breast
cancercell growth in vitro and in vivo.
Biochem Biophys Res Commun. 2012 May 4;421(2):396-402.
178.Wang Y, Xie S, Liu C, Wu Y, Liu Y, Cai Y.
Inhibitory effect of liquiritigenin on migration via downregulation proMMP-2 and
PI3K/Akt signalingpathway in human lung adenocarcinoma A549 cells.
Nutr Cancer. 2012;64(4):627-34.
179.Song Z, Li Y, Liu Y, Xin J, Zou X, Sun W.
α-Enolase, an Adhesion-Related Factor of Mycoplasma bovis.
PLoS One. 2012;7(6):e38836. Epub 2012 Jun 13.
180.Liu W, Li N.
Chicken sine oculis binding protein homolog (sobp), a novel gene that may regulate
feather development.
Poult Sci. 2012 Aug;91(8):1950-5.
181.Ding Y, Huang X, Liu T, Fu Y, Tan Z, Zheng H, Zhou T, Dai J, Xu W.
The Plasmodium Circumsporozoite Protein, a Novel NF-κB Inhibitor, Suppresses the Growth
ofSW480.
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200.Yin Y, Liu W, Ji G, Dai Y.
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207.Wang Q, Luo C, Wu X, Du H, Song X, Fan Y.
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210.Jia F, Fan J, Zhang B, Yuan Z.
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212.Hu J, Wu Q, Li T, Chen Y, Wang S.
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213.Yu SY, Zhang M, Luo J, Zhang L, Shao Y, Li G.
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215.Wang J, Cui J, Zhu H.
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216.Wang Y, Li B, Li Z, Huang S, Wang J, Sun R.
Improvement of hypoxia-ischemia-induced white matter injury in immature rat brain by
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217.Hong JP, Li XM, Li MX, Zheng FL.
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218.Wang J, Zeng L, Zhou Q, Xu Y, Pu S, Jiang W, Zhang X, Du D.
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219.Feng Q, Gou XJ, Meng SX, Huang C, Zhang YQ, Tang YJ, Wang WJ, Xu L, Peng JH, Hu YY.
Qushi Huayu Decoction Inhibits Hepatic Lipid Accumulation by Activating AMP-Activated
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220.Yan Y, Liu H, Wen H, Jiang X, Cao X, Zhang G, Liu G.
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221.Qiu L, Chen Y, Gao M, Zheng C, Zhao Q.
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222.Chen Y, Huang C, Bai C, Gao H, Ma R, Liu X, Dong Q.
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223.Zou M, Xia S, Zhuang L, Han N, Chu Q, Chao T, Peng P, Chen Y, Gui Q, Yu S.
Knockdown of the Bcl-2 gene increases sensitivity to EGFR tyrosine kinase inhibitors in
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224.Jing J, Liu H, Chen H, Hu S, Xiao K, Ma X.
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225.Xue H, Zhang XY, Liu JM, Song Y, Liu TT, Chen D.
NDGA reduces secondary damage after spinal cord injury in rats via anti-inflammatory
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226.Wei Q, Shi F.
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Anim Reprod Sci.2013 May;138(3-4):282-91.doi: 10.1016/j.anireprosci.2013.02.025.
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227.Li L, Ye XP, Lu AZ, Zhou SQ, Liu H, Liu ZJ, Jiang S, Xu SY.
Hyperglycemia magnifies bupivacaine-induced cell apoptosis triggered by mitochondria
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228.Zhang H, Wang ZW, Wu HB, Li Z, Li LC, Hu XP, Ren ZL, Li BJ, Hu ZP.
Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat
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230.Xu T, Wang X, Chen G, He Y, Bie P.
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231.Yan T, Liu Y, Cui K, Hu B, Wang F, Zou L.
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Int Immunopharmacol.2013 May;16(1):7-16.doi: 10.1016/j.intimp.2013.03.010.
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234.Zhang JX, Yang JR, Chen GX, Tang LJ, Li WX, Yang H, Kong X.
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235.Yan M, Shi Y, Wang Y, Wang C, Zhou J, Quan C, Liu C, Yang K.
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236.Li LL, Wang H.
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239.Yang T, Li C, Zhang L, Li M, Zhou P.
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240.Tang Y, Wang YL, Yang L, Xu JX, Xiong W, Xiao M, Li M.
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242.Tang XQ, Zhuang YY, Zhang P, Fang HR, Zhou CF, Gu HF, Zhang H, Wang CY.
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243.Tang Z, Wu M, Li Y, Zheng X, Liu H, Cheng X, Xu L, Wang G, Hao H.
Absolute quantification of NAD(P)H:quinone oxidoreductase 1 in human tumor cell lines
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244.Zou Y, Hong B, Fan L, Zhou L, Liu Y, Wu Q, Zhang X, Dong M.
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245.Hu L, Wu QQ, Wang WB, Jiang HG, Yang L, Liu Y, Yu HJ, Xie CH, Zhou YF, Zhou FX.
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246.Ding J, Li QY, Yu JZ, Wang X, Lu CZ, Ma CG, Xiao BG.
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247.Qing Y, Liang Y, Du Q, Fan P, Xu H, Xu Y, Shi N.
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248.Wang M, Wang J, Tan R, Wu Q, Qiu H, Yang J, Jiang Q.
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249.Chen S, Lin G, Lei L, You X, Wu C, Xu W, Huang M, Luo L, Wang Z, Li Y, Zhao X, Yan F.
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250.Sui Z, Yuan H, Liang Z, Zhao Q, Wu Q, Xia S, Zhang L, Huo Y, Zhang Y.
An activity-maintaining sequential protein extraction method for bioactive assay and
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251.Chen X, Gu M, Zhao X, Zheng X, Qin Y, You X.
Deterioration of cardiac function after acute myocardial infarction is prevented by
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254.Qiu X, Cao L, Yang X, Zhao X, Liu X, Han Y, Xue Y, Jiang H, Chi Z.
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255.Gao M, Wu M, Song Y, Jin L, Lou J, Tao H.
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256.Huo K, Zhang X, Wang H, Zhao L, Liu X, Chu PK.
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257.Han HY, Zhu SH, Jiang LL, Li Y, Dong H, Zhao QP, Kong CL, Huang B.
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259.Zhang E, Yan X, Zhang M, Chang X, Bai Z, He Y, Yuan Z.
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260.Wu W, Yang J, Feng X, Wang H, Ye S, Yang P, Tan W, Wei G, Zhou Y.
MicroRNA-32 (miR-32) regulates phosphatase and tensin homologue (PTEN) expression and
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DICO, a novel nonaromatic B-ring flavonoid, induces G2/M cell cycle arrest and apoptosis
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265.Gu Y,Zhao Y, Gong L, Wang Y, Zhang Y.
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266.Hu X, Yang T, Li C, Zhang L, Li M, Huang W, Zhou P.
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