RIPA裂解液(强)

 产品编号: P0013B
 产品包装: 100ml
 产品价格: 192.00元
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产品简介

产品简介:

产品编号 产品名称 产品包装 产品价格
P0013B RIPA裂解液(强) 100ml 192.00元

    碧云天生产的RIPA裂解液(RIPA Lysis Buffer)是一种传统的细胞组织快速裂解液。RIPA裂解液裂解得
到的蛋白样品可以用于常规的Western、IP等。
    RIPA的本意是Radio Immunoprecipitation AssayRIPA裂解液的配方有很多种,根据其裂解液的强度
大致可以分为强、中、弱三类。关于不同的RIPA裂解液以及碧云天生产的其它裂解液的主要特点和差异,以及如何选择裂解液可参考我们的相关网页:http://www.beyotime.com/lysis-buffer.htm
    RIPA裂解液(强)的主要成分为50mM Tris(pH 7.4),150mM NaCl,1% Triton X-100,1% sodium
deoxycholate,0.1% SDS,以及sodium orthovanadate,sodium fluoride,EDTA,leupeptin等多种抑制剂。可以有效抑制蛋白降解。
    用RIPA裂解液裂解得到的蛋白样品,可以用碧云天生产的BCA蛋白浓度测定试剂盒(P0009/P0010/P0010S
/P0011/P0012/P0012S)
测定蛋白浓度。由于含有较高浓度的去垢剂,不能用Bradford法测定由本裂解液裂解得到样品的蛋白浓度。
包装清单:

产品编号

产品名称

包装

P0013B

RIPA裂解液(强)

100ml

说明书

1份

保存条件:
    -20℃保存,一年有效。
注意事项:
    为取得最佳的使用效果,尽量避免过多的反复冻融。可以适当分装后使用。
    需自备PMSFPMSF(ST506)可以向碧云天订购。
    裂解样品的所有步骤都需在冰上或4℃进行。
    关于裂解液的选择,一方面可以参考碧云天的相关网页:http://www.beyotime.com/lysis-buffer.htm 选择合适的裂解液;另一方面也需要通过一些预实验来摸索最佳的适合您实验条件的裂解液。
    本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于
普通住宅内。
    为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明

使用说明:
对于培养细胞样品:
1. 融解RIPA裂解液,混匀。取适当量的裂解液,在使用前数分钟内加入PMSF,使PMSF的最终浓度为
   1mM
2. 对于贴壁细胞:去除培养液,用PBS、生理盐水或无血清培养液洗一遍(如果血清中的蛋白没有干
   扰,可以不洗)。按照6孔板每孔加入150-250微升裂解液的比例加入裂解液。用枪吹打数下,使裂解
   液和细胞充分接触。通常裂解液接触细胞1-2秒后,细胞就会被裂解。
   对于悬浮细胞:离心收集细胞,用手指把细胞用力弹散。按照6孔板每孔细胞加入150-250微升裂解
   液的比例加入裂解液。再用手指轻弹以充分裂解细胞。充分裂解后应没有明显的细胞沉淀。如果细胞
   量较多,必需分装成50-100万细胞/管,然后再裂解。
3. 充分裂解后,10000-14000g离心3-5分钟,取上清,即可进行后续的PAGE、Western和免疫沉淀等操
   作。
   裂解液用量说明:通常6孔板每孔细胞加入150微升裂解液已经足够,但如果细胞密度非常高可
   以适当加大裂解液的用量到200微升或250微升。
对于组织样品:
1. 把组织剪切成细小的碎片。
2. 融解RIPA裂解液,混匀。取适当量的裂解液,在使用前数分钟内加入PMSF,使PMSF的最终浓度为
   1mM
3. 按照每20毫克组织加入150-250微升裂解液的比例加入裂解液。(如果裂解不充分可以适当添加更多的
   裂解液,如果需要高浓度的蛋白样品,可以适当减少裂解液的用量。)
4. 用玻璃匀浆器匀浆,直至充分裂解。
5. 充分裂解后,10000-14000g离心3-5分钟,取上清,即可进行后续的PAGE、Western和免疫沉淀等操
   作。
6. 如果组织样品本身非常细小,可以适当剪切后直接加入裂解液裂解,通过强烈vortex使样品裂解充
   分。然后同样离心取上清,用于后续实验。直接裂解的优点是比较方便,不必使用匀浆器,缺点是不
   如使用匀浆器那样裂解得比较充分。
注:RIPA裂解液的裂解产物中经常会出现一小团透明胶状物,属正常现象。该透明胶状物为含有基因组
    DNA等的复合物。在不检测和基因组DNA结合特别紧密的蛋白的情况下,可以直接离心取上清用于后
    续实验;如果需要检测和基因组结合特别紧密的蛋白,则可以通过超声处理打碎打散该透明胶状
    物,随后离心取上清用于后续实验。如果检测一些常见的转录因子,例如NF-kappaB、p53等时,通
    常不必进行超声处理,就可以检测到这些转录因子。

附录:碧云天生产的各种裂解液主要特点、差异和选择
首先请参考下表,了解各种裂解液的主要特点和差异。

产品编号

P0013

P0013B

P0013C

P0013D

P0013F

P0013G

P0013J

P0013K

产品名称

Western及IP细胞裂解液

RIPA裂解液(强)

RIPA裂解液(中)

RIPA裂解液(弱)

NP-40裂解液

SDS裂解液

Western及IP细胞裂解液(无抑制剂)

RIPA裂解液(强,无抑制剂)

有效裂解成分

1% Triton X-100

1% Triton X-100, 1% deoxycholate, 0.1% SDS

1% NP-40, 0.5% deoxycholate, 0.1% SDS

1% NP-40, 0.25% deoxycholate

1% NP-40

1% SDS

1% Triton X-100

1% Triton X-100, 1% deoxycholate, 0.1% SDS

裂解强度

温和

温和

温和

温和

对膜蛋白的提取

一般

很好

较好

一般

一般

很好

一般

很好

对胞浆蛋白的提取

很好

很好

很好

很好

很好

很好

很好

很好

对核蛋白的提取

较好

很好

较好

较好

较好

很好

较好

很好

胞浆磷酸化蛋白提取

很好

很好

很好

很好

很好

很好

很好

很好

细胞核转录因子提取

很好

很好

很好

很好

很好

很好

很好

很好

含蛋白酶抑制剂

含磷酸酯酶抑制剂

主要用途

WB, IP,co-IP

WB, IP

WB, IP

WB, IP, co-IP

WB, IP,co-IP

WB, ChIP

WB, IP,co-IP

WB, IP

    用于普通的Western、IP或co-IP,我们推荐使用Western及IP细胞裂解液(P0013),该裂解液已被国内各大研究机构广泛使用,发表大量SCI论文,用户普遍反映很好。裂解细胞或组织后,没有非常粘滞的透明状DNA团块形成,不必采用超声处理等就可以非常理想地用于后续操作。另外该裂解液裂解的产物也适合用于磷酸化蛋白的Western检测。
    对于某些特殊蛋白的IP,如果发现Western及IP细胞裂解液(P0013)效果不是非常理想,可以尝试用RIPA裂解液(强、中或弱)或NP-40裂解液。如果发现IP的时候背景很高,即非特异的蛋白也被IP下来,则需要选用裂解强度较高的裂解液,例如RIPA裂解液(强或中)。如果发现目的蛋白无法被IP下来,则说明裂解液的强度过强,可以使用较为温和的裂解液例如RIPA裂解液(弱)或NP-40裂解液。
    对于某些难溶解蛋白的Western,如果发现Western及IP细胞裂解液(P0013)效果不是非常理想,可以尝试使用裂解强度更高的裂解液例如RIPA裂解液(强、中)或SDS裂解液。使用RIPA裂解液(强)的用户也非常多,发表了大量SCI论文。
    用于特定用途需要自行添加特定抑制剂或不需要添加抑制剂时,可以考虑选购P0013JP0013K
P0013J
在很多时候可以兼容酶活性和生物小分子的检测,对于特定的酶或生物小分子的检测是否兼容需 要自行测试,碧云天不提供具体的应用信息。P0013J的裂解能力比P0013K弱一些,但用于酶活性和生物小分子时,P0013J的兼容性通常会更好一些。

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   Zileuton reduces inflammatory reaction and brain damage following permanent cerebral
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   Inflammation. 2010;33(5):344-52.
53.Sheng R, Gu ZL, Xie ML, Zhou WX, Guo CY.
   Epigallocatechin gallate protects H9c2 cardiomyoblasts against hydrogen dioxides-
   induced apoptosis and telomere attrition.

   Eur J Pharmacol. 2010;641(2-3):199-206. Epub 2010 Jun 8.
54.Wang X, Xu J, Ju S, Ni H, Zhu J, Wang H.
   Livin gene plays a role in drug resistance of colon cancer cells.
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55.Wu W, Yan C, Gan T, Chen Z, Lu X, Duerksen-Hughes PJ, Zhu X, Yang J.
   Nuclear proteome analysis of cisplatin-treated HeLa cells.
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56.Wang S, Lv X, Zhang K, Lin T, Liu X, Yuan J, Dai Y, Li N.
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57.Wan YJ, Li YY, Xia W, Chen J, Lv ZQ, Zeng HC, Zhang L, Yang WJ, Chen T, Lin Y, Wei J, Xu
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   Toxicology. 2010;274(1-3):57-64. Epub 2010 May 26.
58.J. Dang, R.Jia, Y.Tu, S.Xiao, G.Ding.
   Erythropoietin prevents reactive oxygen species generation and renal tubular cell
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59.Liu Y, He R.
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60.Dong Y, Cao J, Wang H, Zhang J, Zhu Z, Bai R, Hao H, He X, Fan R, Dong C.
   Nitric oxide enhances the sensitivity of alpaca melanocytes to respond to
   alpha-melanocyte-stimulating hormone by up-regulating melanocortin-1 receptor.

   Biochem Biophys Res Commun. 2010;396(4):849-53. Epub 2010 May 6.
61.Zhao XL, Li GZ, Sun B, Zhang ZL, Yin YH, Tian YS, Li H, Li HL, Wang de S, Zhong D.
   MMP-mediated cleavage of beta-dystroglycan in myelin sheath is involved in autoimmune
   neuritis.

   Biochem Biophys Res Commun. 2010;392(4):551-6. Epub 2010 Jan 25.
62.Wen L, Liu J, Chen Y, Wu D.
   Identification and preliminary functional analysis of alternative splicing of Siah1 in
   Xenopus laevis.

   Biochem Biophys Res Commun. 2010;396(2):419-24. Epub 2010 Apr 22.
63.Cai J, Yu C, Liu Y, Chen S, Guo Y, Yong J, Lu W, Ding M, Deng H.
   Generation of homogeneous PDX1(+) pancreatic progenitors from human ES cell-derived
   endoderm cells.

   J Mol Cell Biol. 2010;2(1):50-60. Epub 2009 Nov 12.
64.He Y, Shang X, Sun J, Zhang L, Zhao W, Tian Y, Cheng H, Zhou R.
   Gonadal apoptosis during sex reversal of the rice field eel: implications for an
   evolutionarilyconserved role of the molecular chaperone heat shock protein 10.

   J Exp Zool B Mol Dev Evol. 2010 Jun 15;314(4):257-66.
65.Tang WJ, Wang LF, Xu XY, Zhou Y, Jin WF, Wang HF, Gao J
   Autocrine/paracrine action of vitamin D on FGF23 expression in cultured rat osteoblasts.
   Calcif Tissue Int. 2010 May;86(5):404-10.
66.Huang XN, Wang WZ, Fu J, Wang HB.
   The relationship between aquaporin-4 expression and blood-brain and spinal cord
   barrierpermeability following experimental autoimmune encephalomyelitis in the rat.

   Anat Rec (Hoboken). 2011 Jan;294(1):46-54.
67.Liu H, Sun H, Liu C.
   Interference of the apoptotic signaling pathway in RGC stress response by SP600125 in
   moderateocular hypertensive rats.

   Chin J Physiol. 2011 Apr 30;54(2):124-32.
68.Liu N, Huang H, Lin F, Chen A, Zhang Y, Chen R, Du H
   Effects of treadmill exercise on the expression of netrin-1 and its receptors in rat
   brain aftercerebral ischemia.

   Neuroscience. 2011 Oct 27;194:349-58.
69.Lin F, Wang X, Jie Z, Hong X, Li X, Wang M, Yu Y.
   Inhibitory effects of miR-146b-5p on cell migration and invasion of pancreatic cancer by
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   J Huazhong Univ Sci Technolog Med Sci. 2011 Aug;31(4):509-14.
70.Xiao HB, Cao X, Wang L, Run XQ, Su Y, Tian C, Sun SG, Liang ZH
   1,5-dicaffeoylquinic acid protects primary neurons from amyloid β 1-42-induced
   apoptosis viaPI3K/Akt signaling pathway.

   Chin Med J (Engl). 2011 Sep;124(17):2628-35.
71.Lu B, Xu Y, Xu L, Cong X, Yin L, Li H, Peng J.
   Mechanism investigation of dioscin against CCl(4)-induced acute liver damage in mice.
   Environ Toxicol Pharmacol. 2012 Mar 30;34(2):127-135.
72.Ma Y, Niu R, Sun Z, Wang J, Luo G, Zhang J, Wang J
   Inflammatory responses induced by fluoride and arsenic at toxic concentration in rabbit
   aorta.

   Arch Toxicol. 2012 Jun;86(6):849-56.
73.Yang XL, Guo TK, Wang YH, Huang YH, Liu X, Wang XX, Li W, Zhao X, Wang LP, Yan S, Wu D,
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   Ginsenoside Rd attenuates the inflammatory response via modulating p38 and JNK
   signalingpathways in rats with TNBS-induced relapsing colitis.

   Int Immunopharmacol. 2012 Feb;12(2):408-14.
74.Mao SS, Hua R, Zhao XP, Qin X, Sun ZQ, Zhang Y, Wu YQ, Jia MX, Cao JL, Zhang YM.
   Exogenous Administration of PACAP Alleviates Traumatic Brain Injury in Rats through a
   Mechanism Involving the TLR4/MyD88/NF-κB Pathway.

   J Neurotrauma. 2012 Jul 1;29(10):1941-59. doi: 10.1089/neu.2011.2244.
75.Hao M, Kuang HY, Fu Z, Gao XY, Liu Y, Deng W
   Exenatide prevents high-glucose-induced damage of retinal ganglion cells through a
   mitochondrialmechanism.

   Neurochem Int. 2012 Jul;61(1):1-6.
76.Chen J, Lin M, Li N, Lin L, She F
   Therapeutic vaccination with Salmonella-delivered codon-optimized outer inflammatory
   proteinDNA vaccine enhances protection in Helicobacter pylori infected mice.

   Vaccine. 2012 Aug 3;30(36):5310-5.
77.Wang SB, Shi Q, Xu Y, Xie WL, Zhang J, Tian C, Guo Y, Wang K, Zhang BY, Chen C, Gao C,
   Dong XP
   Protein disulfide isomerase regulates endoplasmic reticulum stress and the apoptotic
   processduring prion infection and PrP mutant-induced cytotoxicity.

   PLoS One. 2012;7(6):e38221.
78.Gao Y, Zhang RR, Li JH, Ren M, Ren ZX, Shi JH, Pan QZ, Ren SP.
   Radix Astragali lowers kidney oxidative stress in diabetic rats treated with insulin.
   Endocrine. 2012 Apr 20. [Epub ahead of print]
79.Chen H, Ma N, Xia J, Liu J, Xu Z.
   β2-Adrenergic receptor-induced transactivation of epidermal growth factor receptor and
   platelet-derived growth factor receptor via Src kinase promotes rat cardiomyocyte
   survival.

   Cell Biol Int. 2012 Mar 1;36(3):237-44.
80.Zhang Y, Zhou L, Zhang X, Bai J, Shi M, Zhao G.
   Ginsenoside-Rd attenuates TRPM7 and ASIC1a but promotes ASIC2a expression in rats after
   focalcerebral ischemia.

   Neurol Sci. 2012 Jan 10.
81.Qian Y, Guan T, Huang M, Cao L, Li Y, Cheng H, Jin H, Yu D
   Neuroprotection by the soy isoflavone, genistein, via inhibition of
   mitochondria-dependentapoptosis pathways and reactive oxygen induced-NF-κB activation
   in a cerebral ischemia mouse model.

   Neurochem Int. 2012 Jun;60(8):759-67.
82.Yang XH, Wang YH, Wang JJ, Liu YC, Deng W, Qin C, Gao JL, Zhang LY.
   Role of angiotensin-converting enzyme (ACE and ACE2) imbalance on tourniquet-induced
   remotekidney injury in a mouse hindlimb ischemia-reperfusion model.

   Peptides. 2012 Jul;36(1):60-70. Epub 2012 May 8.
83.Jia LQ, Yang GL, Ren L, Chen WN, Feng JY, Cao Y, Zhang L, Li XT, Lei P.
   Tanshinone IIA reduces apoptosis induced by hydrogen peroxide in the human
   endothelium-derived EA.hy926 cells.

   J Ethnopharmacol. 2012 Jun 28.
84.Wang J, Sun LL, Zhu WL, Sun Y, Liu JF, Lu L, Shi J
   Role of calcineurin in the VTA in rats behaviorally sensitized to methamphetamine.
   Psychopharmacology (Berl). 2012 Mar;220(1):117-28.
85.Wang D, Tang F, Wang S, Jiang Z, Zhang L
   Preclinical anti-angiogenesis and anti-tumor activity of SIM010603, an oral,
   multi-targets receptortyrosine kinases inhibitor.

   Cancer Chemother Pharmacol. 2012 Jan;69(1):173-83.
86.Yu L, Liu Y, Qiu Z, Liu S, Gao X, Zhu D.
   Cellular mechanisms and intracellular signaling pathways for the modulation of eNOS
   inpulmonary arteries by 15-HETE.

   J Recept Signal Transduct Res. 2012 Apr;32(2):87-95.
87.Li J, Guo Y, Liang X, Sun M, Wang G, De W, Wu W
   MicroRNA-223 functions as an oncogene in human gastric cancer by targeting FBXW7/hCdc4.
   J Cancer Res Clin Oncol. 2012 May;138(5):763-74.
88.Liu K, Wang H, Long Y, Ye J, Yuan L
   Coordinate lentiviral expression of Cre recombinase and RFP/EGFP mediated by FMDV 2A
   andanalysis of Cre activity.

   J Cell Biochem. 2012 Sep;113(9):2909-19.
89.Cai L, Bian M, Liu M, Sheng Z, Suo H, Wang Z, Huang F, Fei J.
   Ethanol-induced neurodegeneration in NRSF/REST neuronal conditional knockout mice.
   Neuroscience. 2011 May 5;181:196-205.
90.Deng J, Huang Q, Wang F, Liu Y, Wang Z, Wang Z, Zhang Q, Lei B, Cheng Y
   The role of caveolin-1 in blood-brain barrier disruption induced by focused ultrasound
   combinedwith microbubbles.

   J Mol Neurosci. 2012 Mar;46(3):677-87.
91.Yu X, Tang J, Wang Q, Ye W, Tao K, Duan S, Lu C, Yang X, Dong S, Zheng X, Wang Y
   The RxLR effector Avh241 from Phytophthora sojae requires plasma membrane localization
   toinduce plant cell death.

   New Phytol. 2012 Jul 23.
92.Wang Y, Liu Q, Liu Z, Li B, Sun Z, Zhou H, Zhang X, Gong Y, Shao C
   Berberine, a genotoxic alkaloid, induces ATM-Chk1 mediated G2 arrest in prostate cancer
   cells.

   Mutat Res. 2012 Jun 1;734(1-2):20-9.
93.Liu Y, Xiao Z, Yang D, Ren L, Liu G, Yang L
   Effects of the cyclin-dependent kinase 10 (CDK10) on the tamoxifen sensitivity of keloid
   samples.

   Molecules. 2012 Feb 1;17(2):1307-18.
94.Ding D, Xu H, Liang Q, Xu L, Zhao Y, Wang Y
   Over-expression of Sox2 in C3H10T1/2 cells inhibits osteoblast differentiation through
   Wnt andMAPK signalling pathways.

   Int Orthop. 2012 May;36(5):1087-94.
95.Li R, Yu C, Gao R, Liu X, Lu J, Zhao L, Chen X, Ding Y, Wang Y, He J.
   Effects of DEHP on endometrial receptivity and embryo implantation in pregnant mice.
   J Hazard Mater. 2012 Nov 30;241-242:231-40. doi: 10.1016/j.jhazmat.2012.09.038.
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96.Ding D, Wang Z, Xu H, Xu L, Liang Q, Zhao Y, Shi Q, Wang Y.
   Expression of BMP4 mature peptide in eukaryotic cells and its differentiation-inhibiting
   effect in culturing inducedpluripotent stem cells.

   Nan Fang Yi Ke Da Xue Xue Bao. 2012 Oct;32(10):1383-8.
97.Wang C, Zhang L, He Q, Feng X, Zhu J, Xu Z, Wang X, Chen F, Li X, Dong J.
   Differences in Yes-associated protein and mRNA levels in regenerating liver and
   hepatocellular carcinoma.

   Mol Med Rep. 2012 Feb;5(2):410-4. doi: 10.3892/mmr.2011.640. Epub 2011 Oct 19.
98.Xue B, Wu W, Huang K, Xie T, Xu X, Zhang H, Qi C, Ge J, Yu Y.
   Stromal cell-derived factor-1 (SDF-1) enhances cells invasion by αvβ6 integrin-mediated
   signaling in ovariancancer.

   Mol Cell Biochem. 2013 Aug;380(1-2):177-84. doi: 10.1007/s11010-013-1671-1.
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99.Yang M, Qin Z, Zhu Y, Li Y, Qin Y, Jing Y, Liu S.
   Vitamin D-binding protein in cerebrospinal fluid is associated with multiple sclerosis
   progression.

   Mol Neurobiol. 2013 Jun;47(3):946-56.doi:10.1007/s12035-012-8387-1.Epub 2013 Jan 22.
100.Li G, Zhang S, Fang H, Yan B, Zhao Y, Feng L, Ma X, Ye X.
   Aspirin overcomes Navitoclax-resistance in hepatocellular carcinoma cells through
   suppression of Mcl-1.

   Biochem Biophys Res Commun. 2013 May 17;434(4):809-14.
101.Zhang X, Chen G, Lu Y, Liu J, Fang M, Luo J, Cao Q, Wang X.
   Association of Mitochondrial Letm1 with Epileptic Seizures.
   Cereb Cortex. 2013 May 3. [Epub ahead of print].
102.Chen X, Chen L, Zhang R, Yi Y, Ma Y, Yan K, Jiang X, Wang X.
   ADAM17 regulates self-renewal and differentiation of U87 glioblastoma stem cells.
   Neurosci Lett.2013 Mar 14;537:44-9.doi:10.1016/j.neulet.2013.01.021.Epub 2013 Jan 26.
103.Ye HH, Wu KJ, Fei SJ, Zhang XW, Liu HX, Zhang JL, Zhang YM.
   Propofol participates in gastric mucosal protection through inhibiting the toll-like
   receptor-4/nuclear factor kappa-B signaling pathway.

   Clin Res Hepatol Gastroenterol.2013 Feb;37(1):e3-15.doi:10.1016/
   j.clinre.2012.03.004.Epub 2012 Apr 17.
104.Zheng Y, He L, Wan Y, Song J.
   H3K9me-enhanced DNA hypermethylation of the p16INK4a gene: an epigenetic signature for
   spontaneoustransformation of rat mesenchymal stem cells.

   Stem Cells Dev.2013 Jan 15;22(2):256-67.doi:10.1089/scd.2012.0172.Epub 2012 Nov 7.
105.Liu X, Wang M, Chen H, Guo Y, Ma F, Shi F, Bi Y, Li Y.
   Hypothermia protects the brain from transient global ischemia/reperfusion by attenuating
   endoplasmic reticulum response-induced apoptosis through CHOP.

   PLoS One. 2013;8(1):e53431. doi: 10.1371/journal.pone.0053431. Epub 2013 Jan 3.
106.Liang X, Cheng S, Jiang X, He X, Wang Y, Jiang Z, Hou W, Li S, Liu Y, Wang Z.
   The noncircadian function of the circadian clock gene in the regulation of male
   fertility.

   J Biol Rhythms. 2013 Jun;28(3):208-17. doi: 10.1177/0748730413486873.
107.Wei Q, Ren X, Jiang Y, Jin H, Liu N, Li J.
   Advanced glycation end products accelerate rat vascular calcification through
   RAGE/oxidative stress.

   BMC Cardiovasc Disord. 2013 Mar 5;13:13. doi: 10.1186/1471-2261-13-13.
108.Shou Y, Yang Y, Xu MS, Zhao YQ, Ge LB, Zhang BM.
   Electroacupuncture inhibition of hyperalgesia in rats with adjuvant arthritis:
   involvement of cannabinoid receptor1 and dopamine receptor subtypes in striatum.

   Evid Based Complement Alternat Med. 2013;2013:393460.
109.Gao L, Song JR, Zhang JW, Zhao X, Zhao QD, Sun K, Deng WJ, Li R, Lv G, Cheng HY,
   Wei LX.
   Chloroquine promotes the anticancer effect of TACE in a rabbit VX2 liver tumor model.
   Int J Biol Sci. 2013;9(4):322-30. doi: 10.7150/ijbs.5925. Epub 2013 Mar 28.
110.Zheng XF, Li B, Zhang YH, Yang YH, Meng XY, Jiang SD, Jiang LS.
   Blockade of substance P receptor attenuates osteoporotic pain, but not bone loss, in
   ovariectomized mice.

   Menopause. 2013 Apr 1.
111.Liu W, Otkur W, Li L, Wang Q, He H, Zang L, Hayashi T, Tashiro S, Onodera S, Xia M,
   Ikejima T.
   Interference of silibinin with IGF-1R signalling pathways protects human epidermoid
   carcinoma A431 cells fromUVB-induced apoptosis.

   Biochem Biophys Res Commun. 2013 Mar 8;432(2):314-9. doi: 10.1016/
   j.bbrc.2013.01.109. Epub 2013 Feb 8.
112.Yuan L, Zhang L, Dong X, Zhao H, Li S, Han D, Liu X.
   Apoptin selectively induces the apoptosis of tumor cells by suppressing the
   transcription of HSP70.

   Tumour Biol. 2013 Feb;34(1):577-85. doi: 10.1007/s13277-012-0585-y. Epub 2012 Nov 22.
113.Pang X, Wei Y, Zhang Y, Zhang M, Lu Y, Shen P.
   Peroxisome proliferator-activated receptor-γ activation inhibits hepatocellular
   carcinoma cell invasion byupregulating plasminogen activator inhibitor-1.

   Cancer Sci. 2013 Jun;104(6):672-80. doi: 10.1111/cas.12143. Epub 2013 Apr 8.
114.Xu J, Jia YY, Chen SR, Ye JT, Bu XZ, Hu Y, Ma YZ, Guo JL, Liu PQ.
   (E)-1-(4-ethoxyphenyl)-3-(4-nitrophenyl)-prop-2-en-1-one suppresses LPS-induced
   inflammatory responsethrough inhibition of NF-κB signaling pathway.

   Int Immunopharmacol. 2013 Apr;15(4):743-51. doi: 10.1016/j.intimp.2013.02.024.
   Epub 2013 Mar 14.
115.Xu P, Zhang XX, Miao C, Fu Z, Li Z, Zhang G, Zheng M, Liu Y, Yang L, Wang T.
   Promotion of melanoma cell invasion and tumor metastasis by microcystin-LR via
   phosphatidylinositol 3-kinase/AKT pathway.

   Environ Sci Technol. 2013 Jun 26.
116.Han J, Ding R, Zhao D, Zhang Z, Ma X.
   Unfractionated heparin attenuates lung vascular leak in a mouse model of sepsis:Role of
   RhoA/Rho kinasepathway.

   Thromb Res. 2013 Apr 5. pii: S0049-3848(13)00097-2.
117.Jin J, Zhao WC, Yuan F.
   CXCR7/CXCR4/CXCL12 Axis Regulates the Proliferation, Migration, Survival and Tube
   Formation of Choroid-Retinal Endothelial Cells.

   Ophthalmic Res. 2013;50(1):6-12. doi: 10.1159/000348532. Epub 2013 Mar 21.
118.Wang Q, Li LH, Gao GD, Wang G, Qu L, Li JG, Wang CM.
   HIF-1α up-regulates NDRG1 expression through binding to NDRG1 promoter, leading to
   proliferation of lungcancer A549 cells.

   Mol Biol Rep. 2013 May;40(5):3723-9. doi: 10.1007/s11033-012-2448-4. Epub 2013 Mar 25.
119.Wang J, Wang X, Wu G, Hou D, Hu Q.
   MiR-365b-3p, down-regulated in retinoblastoma, regulates cell cycle progression and
   apoptosis of humanretinoblastoma cells by targeting PAX6.

   FEBS Lett. 2013 Jun 19;587(12):1779-86. doi: 10.1016/j.febslet.2013.04.029.
   Epub 2013 May 6.
110.Wang J, Huang H, Wang C, Liu X, Hu F, Liu M.
   MicroRNA-375 sensitizes tumour necrosis factor-alpha (TNF-α)-induced apoptosis in head
   and neck squamouscell carcinoma in vitro.

   Int J Oral Maxillofac Surg. 2013 Aug;42(8):949-55. doi: 10.1016/j.ijom.2013.04.016.
121.Li A, Lu D, Zhang Y, Li J, Fang Y, Li F, Sun J.
   Critical role of aquaporin-3 in epidermal growth factor-induced migration of colorectal
   carcinoma cells and itsclinical significance.

   Oncol Rep. 2013 Feb;29(2):535-40. doi: 10.3892/or.2012.2144. Epub 2012 Nov 15.
122.Mu H, Yao RB, Zhao LJ, Shen SY, Zhao ZM, Cai H.
   Sinomenine Decreases MyD88 Expression and Improves Inflammation-Induced Joint Damage
   Progression andSymptoms in Rat Adjuvant-Induced Arthritis.

   Inflammation. 2013 Apr 21.
123.Ru R, Yao Y, Yu S, Yin B, Xu W, Zhao S, Qin L, Chen X.
   Targeted genome engineering in human induced pluripotent stem cells by penetrating
   TALENs.

   Cell Regeneration 2013, 2:5 doi:10.1186/2045-9769-2-5.
124.Xu D, Zhang T, Chen X, Zhou Q, Liu C, Deng Z, Zhang L, Ying C, Zhang W, Gu M.
   Reduction of osteopontin in vivo inhibits tubular epithelial to mesenchymal
   transition in rats with chronic allograftnephropathy.

   Transplant Proc. 2013 Mar;45(2):659-65. doi: 10.1016/j.transproceed.2012.02.048.
125.Zhang RX, Yan XB, Gu YH, Huang D, Gan L, Han R, Huang LH.
   Gene silencing of NR2B-containing NMDA receptor by intrathecal injection of short
   hairpin RNA reduces formalin-induced nociception in C57BL/6 mouse.

   Int J Neurosci. 2013 May 9.
126.Li H, Zhou RJ, Zhang GQ, Xu JP.
   Clinical significance of RUNX2 expression in patients with nonsmall cell lung cancer: a
   5-year follow-up study.

   Tumour Biol. 2013 Jun;34(3):1807-12. doi: 10.1007/s13277-013-0720-4. Epub 2013 Mar 8.
127.Mo QQ, Chen PB, Jin X, Chen Q, Tang L, Wang BB, Li KZ, Wu P, Fang Y, Wang SX, Zhou JF,
   Ma D, Chen G.
   Inhibition of Hec1 expression enhances the sensitivity of human ovarian cancer cells to
   paclitaxel.

   IActa Pharmacol Sin.2013 Apr;34(4):541-8.doi:10.1038/aps.2012.197.Epub 2013 Mar 11.
128.Li L, Han M, Li S, Wang L, Xu Y.
   Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic
   Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway.

   DNA Cell Biol. 2013 Jun 19.
129.Zhu X, Jiang Y, Shan PF, Shen J, Liang QH, Cui RR, Liu Y, Liu GY, Wu SS, Lu Q, Xie H,
   Liu YS, Yuan LQ, Liao EY.
   Vaspin attenuates the apoptosis of human osteoblasts through ERK signaling pathway.
   Amino Acids. 2013 Mar;44(3):961-8. doi: 10.1007/s00726-012-1425-5. Epub 2012 Nov 8.
130.Xu N, Yuan H, Liu W, Li S, Liu Y, Wan J, Li X, Zhang R, Chang Y.
   Activation of RAW264.7 mouse macrophage cells in vitro through treatment with
   recombinant ricin toxin-binding subunit B: Involvement of protein tyrosine, NF-κB and
   JAK-STAT kinase signaling pathways.

   Int J Mol Med. 2013 Jul 2. doi: 10.3892/ijmm.2013.1426.
131.Liu L, Liu L, Bo T, Li S, Zhu Z, Cui R , Mao D.
   Puerarin Suppress Apoptosis of Human Osteoblasts via ERK Signaling Pathway.
   International Journal of Endocrinology.Volume 2013 (2013), Article ID 786574,
   6 pages.
132.Zhang J, Ni SS, Zhao WL, Dong XC, Wang JL.
   High expression of JMJD6 predicts unfavorable survival in lung adenocarcinoma.
   Tumour Biol. 2013 Apr 18.
133.Fei X, Jiang S, Zhang S, Li Y, Ge J, He B, Goldstein S, Ruiz G.
   Isolation, Culture, and Identification of Amniotic Fluid-Derived Mesenchymal Stem
   Cells.

   Cell Biochem Biophys. 2013 Mar 19.
134.Ding F, Yu L, Wang M, Xu S, Xia Q, Fu G.
   O-GlcNAcylation involvement in high glucose-induced cardiac hypertrophy via ERK1/2 and
   cyclin D2.

   Amino Acids. 2013 May 12.
135.Sun LL, Zhang Y, Liu JF, Wang J, Zhu WL, Zhao LY, Xue YX, Lu L, Shi J.
   Role of melanin-concentrating hormone in the nucleus accumbens shell in rats
   behaviourally sensitized tomethamphetamine.

   Int J Neuropsychopharmacol. 2013 Mar 1:1-14.
136.Peng H, Zhang W, Xiao T, Zhang Y.
   Nlrp4g is an oocyte-specific gene but is not required for oocyte maturation in the
   mouse.

   Reprod Fertil Dev. 2013 Jun 11. doi: 10.1071/RD12409.
137.Guan X, Guan Y.
   Proteomic profile of differentially expressed proteins in the medial prefrontal cortex
   after repeated cocaineexposure.

   Neuroscience. 2013 Apr 16;236:262-70. doi: 10.1016/j.neuroscience.2013.01.039.
   Epub 2013 Jan 30.
138.Cong ZX, Wang HD, Wang JW, Zhou Y, Pan H, Zhang DD, Zhu L.
   ERK and PI3K signaling cascades induce Nrf2 activation and regulate cell viability
   partly through Nrf2 in humanglioblastoma cells.

   Oncol Rep. 2013 Aug;30(2):715-22. doi: 10.3892/or.2013.2485. Epub 2013 May 23.
139.Shen R, Xie F, Shen H, liu Q, Zheng T, Kou X, Wang D, Yang J.
   Negative correlation of LIV-1 and E-cadherin expression in hepatocellular carcinoma
   cells.

   PLoS One. 2013;8(2):e56542. doi: 10.1371/journal.pone.0056542. Epub 2013 Feb 20.
140.Liu E, Kuang Y, He W, Xing X, Gu J.
   Casticin Induces Human Glioma Cell Death through Apoptosis and Mitotic Arrest.
   Cell Physiol Biochem. 2013 Jun 3;31(6):805-814.
141.Ge D, Kong X, Liu W, Zhao J, Su L, Zhang S, Zhang Y, Zhao B, Miao J.
   Phosphorylation and nuclear translocation of integrin β4 induced by a chemical small
   molecule contribute toapoptosis in vascular endothelial cells.

   Apoptosis. 2013 May 16.
142.Yang J, Huang H, Yang H, He X, Jiang X, Shi Y, Alatangaole D, Shi L, Zhou N.
   Specific activation of the G protein-coupled receptor BNGR-A21 by the neuropeptide
   corazonin from the silkworm, Bombyx mori, dually couples to the G(q) and G(s) signaling
   cascades.

   J Biol Chem.2013 Apr 26;288(17):11662-75.doi:10.1074/jbc.M112.441675 Epub 2013 Mar 1.
143.Xu WP, Yi M, Li QQ, Zhou WP, Cong WM, Yang Y, Ning BF, Yin C, Huang ZW, Wang J, Qian H,
   Jiang CF, Chen YX, Xia CY, Wang HY, Zhang X, Xie WF.
   Perturbation of miR-370-LIN28A-NF-κB regulatory circuit contributes to the development
   of hepatocellularcarcinoma.

   Hepatology. 2013 May 31. doi: 10.1002/hep.26541.
144.Zhao Y, Wang MY, Hao K, Chen XQ, Du JZ.
   CRHR1 mediates p53 transcription induced by high altitude hypoxia through ERK 1/2
   signaling in rat hepaticcells.

   Peptides. 2013 Jun;44:8-14. doi: 10.1016/j.peptides.2013.03.023. Epub 2013 Mar 26.
145.Yin C, Wang PQ, Xu WP, Yang Y, Zhang Q, Ning BF, Zhang PP, Zhou WP, Xie WF, Chen WS,
   Zhang X.
   Hepatocyte nuclear factor-4α reverses malignancy of hepatocellular carcinoma through
   regulating miR-134 in theDLK1-DIO3 region.

   Hepatology. 2013 Jun 14. doi: 10.1002/hep.26573.
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147.Chen Z, Yang Y, Yang X, Zhou C, Li F, Lei P, Zhong L, Jin X, Peng G.
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   Neurol Sci. 2013 Jan 25.
148.Fu Y, Zhang P, Ge J, Cheng J, Dong W, Yuan H, Du Y, Yang M, Sun R, Jiang H.
   Histone deacetylase 8 suppresses osteogenic differentiation of bone marrow stromal cells
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   Int J Biochem Cell Biol. 2014 Sep;54:68-77. doi: 10.1016/j.biocel.2014.07.003. Epub
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149.Wang C, Pan Y, Zhang R, Bai B, Chen J, Randeva HS.
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150.Sun H, Wu Y, Fu D, Liu Y, Huang C.
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   Stem Cells. 2014 Jul;32(7):1943-55. doi: 10.1002/stem.1671.
151.Xie Q, Wang Z, Bi X, Zhou H, Wang Y, Gu P, Fan X.
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   Eur J Pain. 2014 May 19. doi: 10.1002/ejp.534.
153.Cao M, Hou D, Liang H, Gong F, Wang Y, Yan X, Jiang X, Wang C, Zhang J, Zen K, Zhang
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154.Jiang T, Yu JT, Zhu XC, Tan MS, Gu LZ, Zhang YD, Tan L.
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   Neurobiol Aging. 2014 Jun;35(6):1243-51. doi: 10.1016/j.neurobiolaging.2013.11.026.
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155.Wang G, Liu X, Meng L, Liu S, Wang L, Li J, Cui C, Meng J, Hu S, Wei Y.
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   Int J Biol Sci. 2014 Jul 17;10(7):798-806. doi: 10.7150/ijbs.9470. eCollection 2014.
156.Tang W, Yang J, Zhang F, Guo H, Peng F, Wang X.
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   Cytotherapy. 2014 Feb;16(2):225-33. doi: 10.1016/j.jcyt.2013.08.009. Epub 2013 Oct 8.
157.Deng Z, Yan F, Jin Q, Li F, Wu J, Liu X, Zheng H.
   Reversal of multidrug resistance phenotype in human breast cancer cells using
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158.Zhu XC, Jiang T, Zhang QQ, Cao L, Tan MS, Wang HF, Ding ZZ, Tan L, Yu JT.
   Chronic Metformin Preconditioning Provides Neuroprotection via Suppression of NF-κB-
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   Mol Neurobiol. 2014 Aug 30.
159.Guo F, Jiang T, Song W, Wei H, Wang F, Liu L, Ma L, Yin H, Wang Q, Xiong L.
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   Through Adiponectin Receptor 1-Mediated Phosphorylation of GSK-3β.

   Mol Neurobiol. 2014 Jun 11.
160.Wu H, Wang K, Liu W, Hao Q.
   PTEN overexpression improves cisplatin-resistance of human ovarian cancer cells through
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   Biochem Biophys Res Commun. 2014 Feb 7;444(2):141-6. doi: 10.1016/j.bbrc.
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161.Guo S, Xu X, Tang Y, Zhang C, Li J, Ouyang Y, Ju J, Bie P, Wang H.
   miR-15a inhibits cell proliferation and epithelial to mesenchymal transition in
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162.Xu J, Ruan L, Shi H.
   eIF2α of Litopenaeus vannamei involved in shrimp immune response to WSSV infection.
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163.Cong ZX, Wang HD, Zhou Y, Wang JW, Pan H, Zhang DD, Zhang L, Zhu L.
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164.Li S, Wang L, Zhao Q, Liu Y, He L, Xu Q, Sun X, Teng L, Cheng H, Ke Y.
   SHP2 Positively Regulates TGFβ1-induced Epithelial-mesenchymal Transition Modulated by
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   J Biol Chem. 2014 Oct 20. pii: jbc.M113.546077.
165.Li Z, Bi X, Wang M, Zhang J, Song J, Shen X, Han J, Fu G, Ye Y.
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166.Chu H, Ding H, Tang Y, Dong Q.
   Erythropoietin protects against hemorrhagic blood-brain barrier disruption through the
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   Lab Invest. 2014 Sep;94(9):1042-53. doi: 10.1038/labinvest.2014.84. Epub 2014 Jun 30.
167.Qian J, Wu C, Chen X, Li X, Ying G, Jin L, Ma Q, Li G, Shi Y, Zhang G, Zhou N.
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   Cell Signal. 2014 Nov;26(11):2412-23. doi: 10.1016/j.cellsig.2014.07.019. Epub 2014
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168.Wang S, Li Y, Fan J, Wang Z, Zeng X, Sun Y, Song P, Ju D.
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169.Zhu Y, Qin Z, Gao J, Yang M, Qin Y, Shen T, Liu S.
   Vitamin D Therapy in Experimental Allergic Encephalomyelitis Could be Limited by
   Opposing Effects of Sphingosine 1-Phosphate and Gelsolin Dysregulation.

   Mol Neurobiol. 2014 Apr 11.



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