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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| R7072S | Poly(U) Polymerase | 100U | 398.00元 |
| R7072M | Poly(U) Polymerase | 500U | 1598.00元 |
| R7072L | Poly(U) Polymerase | 2kU | 5098.00元 |
碧云天生产的Poly(U) Polymerase，即Poly(U)聚合酶，也称PolyU Polymerase或PolyU聚合酶，简称PUP，是由碧云天自主研发的PerfectProtein™技术平台表达、纯化获得的重组聚合酶。Poly(U) Polymerase能以不依赖模板的方式将来自UTP的UMP整合到底物RNA的3'末端，形成poly(U)尾；也可以不依赖模板的方式将来自ATP的AMP整合到底物RNA的3'末端，形成poly(A)尾，但该酶形成poly(U)尾的能力比形成poly(A)尾的能力强得多。如果反应中使用生物素等标记的UTP，则可实现对RNA的3'末端的生物素标记等[1]。
碧云天Poly(U) Polymerase对RNA 3'末端加Poly(U)尾的效果参考图1。
图1.碧云天Poly(U) Polymerase (R7072)和国外N公司的同类产品(Competitor)对RNA 3'末端加Poly(U)尾的效果图。在25µl反应体系中加入图中指定量的本产品或N公司的Poly(U) Polymerase，37ºC孵育1小时进行反应，65ºC加热20分钟进行失活，反应完毕后加入2X RNA Loading Buffer (R0215)，95ºC孵育3分钟进行变性，随后进行尿素变性聚丙烯酰胺凝胶电泳。如图所示，本产品与N公司的产品相比，具有类似的对RNA 3'末端加Poly(U)尾的效果。反应体系(25μl)：50mM NaCl, 10mM Tris-HCl, 10mM MgCl2, 1mM DTT, pH7.9 @ 25ºC, 0.5mM UTP，人工合成的80pmol的16nt长度的RNA以及不同浓度的Poly(U) Polymerase。实际检测效果会因实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。
用途：RNA 3'端加Poly(U)尾或Poly(A)尾，然后用于克隆；用生物素或荧光修饰的UTP标记RNA；放射性标记RNA；对3'末端为2'氧甲基的RNA进行Poly(A)加尾；对进入真核细胞的RNA加尾从而研究其对RNA稳定性和翻译的影响。
来源：由大肠杆菌表达，表达基因为来自Schizosaccharomyces pombe Poly(U) Polymerase基因。
活性单位定义：One unit is defined as the amount of enzyme that incorporates 1nmol of UMP into RNA in a 50μl volume in 10 minutes at 37ºC.
纯度：不含DNase、RNase和磷酸酯酶。
酶储存溶液：10mM Tris-HCl (pH7.5 @ 25ºC), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% (v/v) Glycerol.
失活或抑制：65ºC加热20分钟可使Poly(U) Polymerase失活。
10X Poly(U) Reaction Buffer：500mM NaCl, 100mM Tris-HCl, 100mM MgCl2, 10mM DTT, pH7.9 @ 25ºC.
包装清单：| 产品编号 | 产品名称 | 包装 |
| R7072S-1 | Poly(U) Polymerase (2U/µl) | 50µl |
| R7072S-2 | 10X Poly(U) Reaction Buffer | 0.2ml |
| R7072S-3 | UTP (10mM) | 65μl |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| R7072M-1 | Poly(U) Polymerase (2U/µl) | 250µl |
| R7072M-2 | 10X Poly(U) Reaction Buffer | 1ml |
| R7072M-3 | UTP (10mM) | 325μl |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| R7072L-1 | Poly(U) Polymerase (2U/µl) | 1ml |
| R7072L-2 | 10X Poly(U) Reaction Buffer | 4ml |
| R7072L-3 | UTP (10mM) | 1.3ml |
| — | 说明书 | 1份 |
-20ºC保存，至少两年有效。
注意事项：10X Poly(U) Reaction Buffer中不包含UTP。
Poly(U) Polymerase的用量主要影响目的产物的长度，可以根据所需目的产物的长度适当调整酶的用量，如果想得到长度较长的产物可以适当加大酶的用量或延长反应时间。
本产品中在使用时宜存放在冰盒内或冰浴上，使用完毕后宜立即放置于-20ºC保存。
超纯水推荐使用BeyoPure™ Ultrapure Water (DNase/RNase-Free, Sterile) (ST876)。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| Component | Volume |
| Ultrapure Water | 18.45µl |
| 10X Poly(U) Reaction Buffer | 2.5µl |
| Single-stranded RNA (100µM) | 0.8µl |
| UTP (10mM) | 1.25µl |
| RNase Inhibitor (R0102) | 1µl |
| Poly(U) Polymerase (2U/µl) | 1µl |
| 产品编号 | 产品名称 | 包装 |
| D7378-250μl | ATP (100mM, Nuclease free) | 250μl |
| D7378-1ml | ATP (100mM, Nuclease free) | 1ml |
| D7378-5ml | ATP (100mM, Nuclease free) | 5ml |
| D7379-250μl | CTP (100mM, Nuclease free) | 250μl |
| D7379-1ml | CTP (100mM, Nuclease free) | 1ml |
| D7379-5ml | CTP (100mM, Nuclease free) | 5ml |
| D7380-250μl | GTP (100mM, Nuclease free) | 250μl |
| D7380-1ml | GTP (100mM, Nuclease free) | 1ml |
| D7380-5ml | GTP (100mM, Nuclease free) | 5ml |
| D7381-250μl | UTP (100mM, Nuclease free) | 250μl |
| D7381-1ml | UTP (100mM, Nuclease free) | 1ml |
| D7381-5ml | UTP (100mM, Nuclease free) | 5ml |
| D7383-1ml | NTP set (100mM each, Nuclease free) | 4×250μl |
| D7385-500μl | NTP Mix (10mM each, Nuclease free) | 500μl |
| D7385-2ml | NTP Mix (10mM each, Nuclease free) | 2ml |
| D7387-250μl | NTP Mix (25mM each, Nuclease free) | 250μl |
| D7387-1ml | NTP Mix (25mM each, Nuclease free) | 1ml |
| D7062 | SP6 RNA Polymerase | 500U |
| D7066 | T3 RNA Polymerase | 500U |
| D7069 | T7 RNA Polymerase | 1000U |
| D7089 | RNase H | 100U |
| D7098S | T4 Polynucleotide Kinase | 500U |
| D7098M | T4 Polynucleotide Kinase | 2000U |
| D7098L | T4 Polynucleotide Kinase | 10000U |
| D7100S | T4 Polynucleotide Kinase (3' phosphatase minus) | 500U |
| D7100M | T4 Polynucleotide Kinase (3' phosphatase minus) | 2000U |
| D7100L | T4 Polynucleotide Kinase (3' phosphatase minus) | 10000U |
| P0347S | RNase活性荧光检测试剂盒 | 100次 |
| P0347M | RNase活性荧光检测试剂盒 | 500次 |
| R0051 | Annealing Buffer for RNA Oligos (5X) | 1ml |
| R0056-2ml | PEG8000 (50%, RNase free) | 2ml |
| R0058-1ml | MgCl2 (100mM, DEPC-treated) | 1ml |
| R0621S | T4 RNA Ligase 1 (ssRNA Ligase) | 1000U |
| R0621M | T4 RNA Ligase 1 (ssRNA Ligase) | 5000U |
| R0632S | T4 RNA Ligase 2 (dsRNA Ligase) | 1000U |
| R0635S | T4 RNA Ligase 2, truncated | 5kU |
| R0635M | T4 RNA Ligase 2, truncated | 20kU |
| R0635L | T4 RNA Ligase 2, truncated | 100kU |
| R0637S | T4 RNA Ligase 2, truncated KQ | 5kU |
| R0637M | T4 RNA Ligase 2, truncated KQ | 20kU |
| R0637L | T4 RNA Ligase 2, truncated KQ | 100kU |
| R0639S | AlkB (RNA/DNA去甲基化酶, Nuclease-free) | 200U |
| R0639M | AlkB (RNA/DNA去甲基化酶, Nuclease-free) | 1000U |
| R0639L | AlkB (RNA/DNA去甲基化酶, Nuclease-free) | 5000U |
| R0700S | 小RNA 3'接头(5'腺苷化, 3'封闭)及连接试剂盒 | 20次 |
| R0702S | Universal miRNA Cloning Linker (5´腺苷化3´封闭) | 1μg |
| R0702M | Universal miRNA Cloning Linker (5´腺苷化3´封闭) | 5μg |
| R0716S | 5' DNA Adenylation Kit | 10次 |
| R0716M | 5' DNA Adenylation Kit | 50次 |
| R0719S | 5' DNA/RNA Adenylase | 3kU |
| R0719M | 5' DNA/RNA Adenylase | 15kU |
| R0722S | Circular RNA Synthesis Kit | 20次 |
| R0722M | Circular RNA Synthesis Kit | 100次 |
| R7006S | SP6 RNA Polymerase | 1KU |
| R7006M | SP6 RNA Polymerase | 5KU |
| R7006L | SP6 RNA Polymerase | 25KU |
| R7006XL | SP6 RNA Polymerase | 100KU |
| R7009S | T3 RNA Polymerase | 1KU |
| R7009M | T3 RNA Polymerase | 5KU |
| R7009L | T3 RNA Polymerase | 25KU |
| R7009XL | T3 RNA Polymerase | 100KU |
| R7012S | T7 RNA Polymerase | 1KU |
| R7012M | T7 RNA Polymerase | 5KU |
| R7012L | T7 RNA Polymerase | 25KU |
| R7012XL | T7 RNA Polymerase | 100KU |
| R7013S | T7 RNA Polymerase (High Concentration) | 25KU |
| R7013M | T7 RNA Polymerase (High Concentration) | 100KU |
| R7013L | T7 RNA Polymerase (High Concentration) | 500KU |
| R7016S | T7 Quick High Yield RNA Transcription Kit | 25次 |
| R7016M | T7 Quick High Yield RNA Transcription Kit | 100次 |
| R7018S | T7 High Yield RNA Transcription Kit | 25次 |
| R7018M | T7 High Yield RNA Transcription Kit | 100次 |
| R7020S | SP6 High Yield RNA Transcription Kit | 25次 |
| R7020M | SP6 High Yield RNA Transcription Kit | 100次 |
| R7070S | E.coli Poly(A) Polymerase | 100U |
| R7070M | E.coli Poly(A) Polymerase | 500U |
| R7070L | E.coli Poly(A) Polymerase | 2.5kU |
| R7070XL | E.coli Poly(A) Polymerase | 10kU |
| R7075S | Poly(A) Polymerase Tailing Kit | 50-250次 |
| R7088S | RNase H | 250U |
| R7088M | RNase H | 1000U |
| R7088L | RNase H | 5000U |
| R7090S | Thermostable RNase H | 250U |
| R7090M | Thermostable RNase H | 1000U |
| R7090L | Thermostable RNase H | 5000U |
| R7090FT | Thermostable RNase H | 50U |
| R7092S | RNase R | 500U |
| R7092M | RNase R | 2KU |
| R7092L | RNase R | 10KU |
| R7096S | RNase T1 | 100000U |
| R7096M | RNase T1 | 500000U |
| R0081-1ml | BeyoMag™ RNA Clean Magnetic Beads (RNA纯化磁珠) | 1ml |
| R0081-5ml | BeyoMag™ RNA Clean Magnetic Beads (RNA纯化磁珠) | 5ml |
| R0081-20ml | BeyoMag™ RNA Clean Magnetic Beads (RNA纯化磁珠) | 20ml |
| R0081-100ml | BeyoMag™ RNA Clean Magnetic Beads (RNA纯化磁珠) | 100ml |
| R0301S | BeyoClick™ EU-488 RNA合成检测试剂盒 | 50-500次 |
| R0301L | BeyoClick™ EU-488 RNA合成检测试剂盒 | 200-2000次 |
| R0305S | BeyoClick™ EU-555 RNA合成检测试剂盒 | 50-500次 |
| R0305L | BeyoClick™ EU-555 RNA合成检测试剂盒 | 200-2000次 |
| R0309S | BeyoClick™ EU-594 RNA合成检测试剂盒 | 50-500次 |
| R0309L | BeyoClick™ EU-594 RNA合成检测试剂盒 | 200-2000次 |
| R0311S | BeyoClick™ EU-647 RNA合成检测试剂盒 | 50-500次 |
| R0311L | BeyoClick™ EU-647 RNA合成检测试剂盒 | 200-2000次 |
| R7061S | Biotin RNA Labeling Kit (SP6/T7) | 20次 |
| R7061M | Biotin RNA Labeling Kit (SP6/T7) | 100次 |
| ST040-1g | DTT (DNase, RNase & Protease free) | 1g |
| ST040-5g | DTT (DNase, RNase & Protease free) | 5g |
| ST040-25g | DTT (DNase, RNase & Protease free) | 25g |
| ST040-100g | DTT (DNase, RNase & Protease free) | 100g |
| ST041-2ml | 0.5M DTT (DNase, RNase & Protease free) | 2ml |
| ST041-10ml | 0.5M DTT (DNase, RNase & Protease free) | 10ml |
| ST576 | RNase A (10mg/ml, DNase free) | 1ml |
| ST577 | RNase A (100mg/ml, DNase free) | 0.5ml |
| ST578 | RNase A (10mg/ml) | 1ml |
| ST579 | RNase A (100mg/ml) | 0.5ml |
| ST876-100ml | BeyoPure™ Ultrapure Water (DNase/RNase-Free, Sterile) | 100ml |
| ST876-500ml | BeyoPure™ Ultrapure Water (DNase/RNase-Free, Sterile) | 500ml |
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